Abcg2 Rat Monoclonal Antibody [Clone ID: BXP-9]

CAT#: AM32027SU-N

Abcg2 rat monoclonal antibody, clone BXP-9, Supernatant

Specifications

Product Data

• Clone Name: BXP-9
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting: Use 1/20-1/50 dilution and HRP-anti-Rat-IgG.
  Flow Cytometry (optimal conditions still to be defined).
  Immunocytochemistry: Use 1/20-1/50 on Acetone Fixed Cytospin preparations.
  Immunohistochemistry on Frozen Sections: 1/20 on Acetone Fixed Frozen Sections can be followed by incubation with Biotin-labeled Rabbit anti-Rat IgG (1/100) and HRP-labeled streptavidin (1/ 500).
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fusion protein containing the E. coli maltose binding protein and a fragment of the Mouse bcrp protein corresponding to amino acids 221-394.
• Specificity:

  This Monoclonal antibody clone BXP-9 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance.
  It does not react with the Human BCRP molecule.

• Formulation: State: Supernatant
  State: Serum Free Culture Supernatant
  Stabilizer: 0.7% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: ATP-binding cassette, sub-family G (WHITE), member 2
• Database Link:
  Entrez Gene 26357 Mouse
  UniProt ID Q7TMS5
• Background: The breast cancer resistance protein (BCRP/ABCG2) is a member of the ATP-binding cassette family of drug transporters and confers resistance to various anticancer drugs. The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
• Synonyms: Breast cancer resistance protein 1, ABCP, MXR