NANOG Mouse Monoclonal Antibody [Clone ID: 60CT70.5.12]

CAT#: AM11049SU-N

NANOG mouse monoclonal antibody, clone 60CT70.5.12, Purified

Product Images

Western Blot analysis of anti-NANOG monoclonal antibody by NONAG recombinant protein. NANOG (NANOG + His tag) (arrow) was detected using the Mab.

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  Western blot analysis of anti-NANOG monoclonal antibody in K562 cell line lysates using (35ug/lane). This demonstrates the NANOG antibody detected the NANOG protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using anti-NANOG monoclonal antibody followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Clone Name: 60CT70.5.12
• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/1,000.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This antibody is generated from mouse immunized with Human recombinant NANOG protein.
• Specificity: This antibody is specific to Human NANOG.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34620 Da
• Gene Name: Nanog homeobox
• Database Link:
  Entrez Gene 79923 Human
  UniProt ID Q9H9S0
• Background: NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
• Synonyms: FLJ12581; FLJ40451; hNanog