PML Protein (PML) Mouse Monoclonal Antibody [Clone ID: B4C8]

CAT#: AM09255PU-N

PML Protein (PML) mouse monoclonal antibody, clone B4C8, Aff - Purified

Product Images

Specifications

Product Data

• Clone Name: B4C8
• Applications: WB
• Recommended Dilution: EIA.
  Western Blot: A106Kd band was observed in K562 cell extract in Western blotting experiment. The K562 cells express the high molecular weight PML/RARa chimera.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant partial human PML
• Specificity: This antibody recognizes normal PML proteins of lower molecular weight, and also recognizes the high molecular weight PML/RARα chimeric protein in promyelocytic leukemia patients.
• Formulation: 0.01M PBS, pH 7.4
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in appropriate volume of distilled H₂O to reach a final antibody concentration of 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: promyelocytic leukemia
• Database Link:
  Entrez Gene 5371 Human
  UniProt ID P29590
• Background: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals.
• Synonyms: RING finger protein 71, MYL, TRIM19

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis