RAF1 Mouse Monoclonal Antibody [Clone ID: 4G4]

CAT#: AM06729PU-N

RAF1 mouse monoclonal antibody, clone 4G4, Purified

Product Images

Western blot analysis using RAF1 antibody Cat.-No AM06729PU-N against human RAF1 (AA: 198-407) recombinant protein (Expected MW is 73 kDa).

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  Western blot analysis using RAF1 antibody Cat.-No AM06729PU-N against Hela (1), A431 (2), Cos7 (3) and C6 (4) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAF1 antibody Cat.-No AM06729PU-N with DAB staining.

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  Flow cytometric analysis of Hela cells using RAF1 antibody Cat.-No AM06729PU-N (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 4G4
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human RAF1 expressed in E. Coli.
• Specificity: This antibody recognizes RAF1
• Formulation: State: Purified
  State: Liquid purified antibody in PBS containing 0.05% Sodium Azide and 0.5% protein stabilizer
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Raf-1 proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 5894 Human
  UniProt ID P04049
• Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
• Synonyms: C-RAF, Raf-1, cRaf
• Note: Tissue Array Results: Hepatic Carcinoma (+, Cytoplasm, B5/6), Skeletal muscle (+, Cytoplasm, H7/8), Liver (+, Cytoplasm, C5/6).