ABCG2 Mouse Monoclonal Antibody [Clone ID: 3G8]

CAT#: AM06628SU-N

ABCG2 mouse monoclonal antibody, clone 3G8, Ascites

Product Images

Western blot analysis using ABCG2 mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.

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  Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).

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Specifications

Product Data

• Clone Name: 3G8
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
  Immunofluorescence: 1/200-1/1000.
  Flow Cytometry: 1/200-1/400.
  ELISA: 1/10000.
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human ABCG2 expressed in E. Coli.
  
• Specificity: This antibody reacts to ABCG2.
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 72 kDa
• Gene Name: ATP binding cassette subfamily G member 2 (Junior blood group)
• Database Link:
  Entrez Gene 9429 Human
  UniProt ID Q9UNQ0
• Background: The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.
• Synonyms: Breast cancer resistance protein 1, ABCP, MXR