WIF1 Mouse Monoclonal Antibody [Clone ID: 1G5]

CAT#: AM06523SU-N

WIF1 mouse monoclonal antibody, clone 1G5, Ascites

Product Images

Western blot analysis using WIF1 antibody Cat.-No AM06523SU-N against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 antibody Cat.-No AM06523SU-N with DAB staining.

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  Immunofluorescence analysis of Hela cells using WIF1 antibody Cat.-No AM06523SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Product Data

• Clone Name: 1G5
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human WIF1 expressed in E. Coli.
• Specificity: recognizes WIF1
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42 kDa
• Gene Name: WNT inhibitory factor 1
• Database Link:
  Entrez Gene 11197 Human
  UniProt ID Q9Y5W5
• Background: The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
• Synonyms: WIF-1, UNQ191/PRO217