SMAD3 Mouse Monoclonal Antibody [Clone ID: 5G11]

CAT#: AM06479SU-N

SMAD3 mouse monoclonal antibody, clone 5G11, Ascites

Product Images

Western blot analysis using SMAD3 antibody Cat.-No AM06479SU-N against A549 (1), Hela (2), Jurkat (3), PC-2 (4) and NIH/3T3 (5) cell lysate.

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  Immunofluorescence analysis of NIH/3T3 cells using SMAD3 antibody Cat.-No AM06479SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Product Data

• Clone Name: 5G11
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human SMAD3 expressed in E. Coli.
• Specificity: Recognizes SMAD3
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 48 kDa
• Gene Name: SMAD family member 3
• Database Link:
  Entrez Gene 4088 Human
  UniProt ID P84022
• Background: SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
• Synonyms: SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2