B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: 1H12F1]

CAT#: AM06152SU-N

B Raf (BRAF) mouse monoclonal antibody, clone 1H12F1, Ascites

Product Images

Western blot analysis using BRAF antibody Cat.-No AM06152SU-N against truncated recombinant Braf (Lane 1) and A431 cell lysate (Lane 2).

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  Western blot analysis using BRAF antibody Cat.-No AM06152SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY Braf cDNA (Lane 2).

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  Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody Cat.-No AM06152SU-N

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  Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue (left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF antibody Cat.-No AM06152SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 1H12F1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of BRAF expressed in E. Coli.
• Specificity: Recognizes BRAF (V-raf murine sarcoma viral oncogene homolog B1).
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: B-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 673 Human
  UniProt ID P15056
• Background: BRAF (V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers.
• Synonyms: BRAF, BRAF1, RAFB1, p94