MSH2 Mouse Monoclonal Antibody [Clone ID: 1B3=3A2B8C]

CAT#: AM06094SU-N

MSH2 mouse monoclonal antibody, clone 1B3=3A2B8C, Ascites

Product Images

Western blot analysis using MSH2 antibody Cat.-No AM06094SU-N against Hela (Lane 1), A549 (Lane 2), A431 (Lane 3) and HEK293 (Lane 4) cell lysate

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  Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 antibody Cat.-No AM06094SU-N with DAB staining.

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  Confocal Immunofluorescence analysis of Hela cells using MSH2 antibody Cat.-No AM06094SU-N (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Product Data

• Clone Name: 1B3=3A2B8C
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human MSH2 expressed in E. Coli.
• Specificity: Recognizes MSH2
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 105 kDa
• Gene Name: mutS homolog 2
• Database Link:
  Entrez Gene 4436 Human
  UniProt ID P43246
• Background: MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
• Synonyms: DNA mismatch repair protein Msh2, MutS protein homolog 2