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Rabbit Polyclonal Anti-6×His Antibody

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SKU Description Datasheet Price Availability  
TA100027 Rabbit Polyclonal Anti-6×His Antibody $325 2 Weeks Add to Shopping Cart
Conjugation is available for this antibody: choose conjugation type
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Specification

Product NameRabbit Polyclonal Anti-6×His Antibody
See all His Antibodies >>
Clone NamePolyclonal
IsoTypeIgG
Concentration1.5 mg/ml
ImmunogenSynthetic peptide: 18×His conjugated to KLH
Host SpeciesRabbit
FormulationpH7.4 PBS, 0.05% NaN3, 40% Glycerol
ReactivityFusion proteins containing the HIS Tag
Validated ApplicationWB, ELISA
Suggested DilutionELISA: 5000-10000, WB: 2000-10000
Predicted Size50 kDa
Storage/StabilityStable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. After reconstitution, aliquot into small quantity for long term storage at -20ºC. Avoid repeated freezing and thawing. Aliquots for short-term usage will be stable at 4ºC for 3 months.
WB Image
Gel: 8%SDS-PAGE, Lysate: 0.5 ug, Lane: Recombinant 6×HIS Tagged protein , Primary antibody: (6×His Antibody) at dilution 1/2000 (lane 1),1/5000 (lane 2),1/8000 (lane 3),1/10000 (lane 4), Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

 

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