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Anti-His tag monoclonal antibody

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Specifications Citations (1) Customer Reviews Product Documents
SKU Description Datasheet Price Availability  
TA100013 Anti-His tag monoclonal antibody $248 In Stock Add to Shopping Cart
Conjugation is available for this antibody: choose conjugation type
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Specification

Product NameAnti-His tag monoclonal antibody
See all His Antibodies >>
Clone Nameclone HIS.H8
IsoTypeIgG2b
Concentration1mg/ml
ImmunogenMonoclonal antibody is produced by immunizing mice with a synthetic peptide (6 x His)
Host SpeciesMouse
FormulationPBS, pH 7.4 with 0.05% sodium azide
ReactivityHis tag
Validated ApplicationWB, IF/ICC
Suggested DilutionWB: 1:1000, IP: 1:200, IF: 1:200
Predicted Size
Storage/StabilityStable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.
WB Image
Western blot analysis of HEK293 cell lysates expressing BLK or BTK tagged by indicated epitopes.
WB Image
Western blot analysis of HEK293 cells transfected with His-tagged vector (2) or untransfected control (1).
IF Image
Immunofluorescence analysis of His-tag fusion protein (red) in 293 cells. Left: untransfected control; Right: transfected. Counterstained with DAPI (blue).
IF Image
Paraformaldehyde-fixed ARPE-19 cells transfected with hBestrophin1-His. Detection using His-tag mouse monoclonal antibody (1:1,000) with anti-mouse Alexa Fluor 546.

 

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