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UltraMAB

PDCD1 UltraMAB Antibody, Clone UMAB199

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800091
  • PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199, 100 ul
$468 In Stock Add to Shopping Cart
UM870091
  • PDCD1 mouse monoclonal antibody,clone UMAB199, 30 ul
$120 In Stock Add to Shopping Cart
UM800091CF
  • Carrier-free (BSA/glycerol-free) PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(9)
Data Img
IF(2)
Data Img
10K-CHIP(1)
Data Img
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Also for PDCD1 (NM_005018)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
Clone NameClone UMAB199 IsotypeIgG2a
Species ReactivityHuman, Mouse, Dog, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsIHC 1:1000, WB 1:500, IF 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens programmed cell death 1 (PDCD1)
Alternative NameCD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
Database LinkNP_005009
FunctionThis gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800091)(1:1200)
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Immunohistochemical staining of 3 paraffin-embedded human lung carcinomas using anti-PD1 clone UMAB199 mouse monoclonal antibody at 1:800 requires HIER Accel [OriGene/GBI Labs B22-125] in Pressure Chamber for 3 minute on high. Detection of primary antibody was achieved with Polink2 Broad HRP DAB [OriGene/GBI Labs D22].
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Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The composite image of 3 melanoma shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded human prostate carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded human colon cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded human endometrial cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
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Sequential double staining of paraffin human lung using anti-b-Catenin UM500015 (red) and anti-PD1 UM800091 (brown). Both antibodies at 1:800 dilution of 1mg/mL. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-Catenin: citrate pH6.0. Image shows tumor cells are strongly positve for b-catenin (red) and negative for PD1. The arrows point to the activated T cells (brown) showing strong membranous and cytoplasmic staining of PD1 and no staining with b-catenin.
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Sequential double staining of paraffin human melanoma using b-catenin UM500015 (red) and PD1 UM800091 (brown). Both abs at 1:800 dilution of 1mg/mL; detection with Polink2 HRP DAB followed by Polink2 Broad AP. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-catenin: citrate pH6.0 . Image shows tumor cells are strongly positve for b-catenin (red) and negative for PD1. The activated T cells (brown) show strong membranous and cytoplasmic staining for PD1 and no staining with b-catenin.
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Immunofluorescent staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse monoclonal antibody UM800091 (1:100, green) and nucleus with Hoechst33342 (blue) . HEK293T cells serve as negative control (right).
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Immunocytochemistry staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody UM800091 (1:900). The rihgt is negative control.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (UM800091). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
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