Oaz3 (NM_001101018) Rat Untagged Clone

CAT#: RN215944

Oaz3 (untagged) - Rat ornithine decarboxylase antizyme 3 (Oaz3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Oaz3
• Synonyms: Az3; Oaz-t; ODC-Az 3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215944 representing NM_001101018
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGCCTTGTACCAGGTCCCGCCCCTCTCTCTACTCCCTTTCTTATATTAAGAGGGGAAAAACACGGAACT
  GCCTCTACCCATTCTGGTCACCATACGCCTATTACCTCTACTGTTACAAATACCGGATCACCCTCCGGGA
  GAAGATGCTGCCTTGTTGTTACAGAAGCATCACTTACAAGGAACAGGAGGACCTGACTCTCCGGCCCCAT
  TGCTGCCTCCCGTGCTCCTGCCTCCCGTACTCCTGCCTCCCGTGCTCCCTGCCTTGTACCAGGTCCCGCC
  CCTCTCTCTACTCCCTTTCTTATATTAAGAGGGGAAAAACACGGAACTGCCTCTACCCATTCTGGTCACC
  ATACGCCTATTACCTCTACTGTTACAAATACCGGATCACCCTCCGGGAGAAGATGCTGCCTTGTTGTTAC
  AGAAGCATCACTTACAAGGAACAGGAGGACCTGACTCTCCGGCCCCATTGCTGCCTCCCGTGCTCCTGCC
  TCCCGTACTCCTGCCTCCCGTGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001101018
• Insert Size: 516 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101018.1, NP_001094488.1
• RefSeq Size: 878 bp
• RefSeq ORF: 733 bp
• Locus ID: 689588
• Cytogenetics: 2q34
• Gene Summary: The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake; however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. This transcript initiates translation from a non-AUG (CUG) codon that is highly conserved among the antizyme 3 orthologs. [provided by RefSeq, Dec 2014]