Akt1 (NM_033230) Rat Untagged Clone

CAT#: RN201042

Akt1 (untagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug)


  "NM_033230" in other vectors (3)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (3)
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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Akt1
Synonyms Akt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN201042 representing NM_033230
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGACGTAGCCATTGTGAAGGAGGGCTGGCTGCACAAACGAGGGGAATATATTAAAACCTGGCGGC
CACGCTACTTCCTCCTCAAGAATGATGGCACCTTTATTGGCTACAAGGAACGGCCTCAGGATGTGGAGCA
GCGCGAGTCCCCACTCAACAACTTCTCAGTGGCACAATGTCAGCTGATGAAGACAGAGCGGCCGAGGCCC
AACACCTTCATCATCCGCTGCCTGCAGTGGACCACGGTCATTGAGCGCACCTTCCATGTGGAAACGCCTG
AGGAGCGGGAAGAGTGGACCACCGCCATTCAGACTGTGGCTGATGGACTCAAACGGCAGGAGGAGGAGAC
GATGGACTTCCGGTCAGGTTCACCCAGTGACAACTCAGGTGCTGAGGAGATGGAGGTGGCCCTGGCCAAG
CCCAAGCACCGTGTGACCATGAACGAGTTTGAGTACCTGAAGCTACTGGGCAAGGGCACCTTTGGGAAGG
TGATCCTGGTGAAGGAGAAGGCCACAGGTCGCTACTATGCCATGAAGATCCTCAAGAAGGAGGTCATCGT
TGCCAAGGATGAGGTTGCCCACACGCTTACTGAGAACCGTGTCCTGCAGAACTCTAGGCATCCCTTCCTT
ACAGCCCTCAAGTACTCATTCCAGACCCACGACCGCCTCTGCTTTGTCATGGAGTACGCCAATGGAGGCG
AGCTCTTCTTCCACCTGTCTCGTGAGCGCGTGTTTTCAGAGGACCGGGCCCGCTTCTACGGTGCGGAGAT
TGTGTCCGCCCTGGACTACTTGCACTCCGAGAAGAACGTGGTGTACCGGGACCTCAAGCTGGAGAACCTC
ATGCTGGACAAGGACGGGCACATCAAGATAACGGACTTCGGGCTGTGCAAGGAGGGTATCAAGGACGGTG
CCACCATGAAGACGTTCTGCGGGACACCCGAGTACCTGGCCCCTGAGGTGCTGGAGGACAACGACTATGG
CCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTAC
AACCAGGACCATGAGAAGCTGTTCGAGCTCATCCTAATGGAGGAGATCCGCTTCCCACGCACACTCGGGC
CGGAGGCCAAGTCCCTGCTCTCGGGGCTGCTCAAGAAGGACCCTACACAGAGGCTCGGTGGGGGCTCCGA
GGACGCCAAGGAGATCATGCAGCACCGCTTCTTTGCCAACATCGTGTGGCAAGATGTGTATGAGAAGAAG
CTGAGCCCACCTTTCAAGCCCCAGGTCACCTCTGAGACCGACACCAGGTATTTTGATGAGGAGTTCACAG
CTCAGATGATCACCATCACACCGCCTGATCAAGATGACAGCATGGAGTGTGTGGACAGTGAACGGAGGCC
GCACTTTCCCCAGTTCTCCTACTCAGCCAGTGGCACAGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_033230
Insert Size 1443 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033230.1, NP_150233.1
RefSeq Size 1613 bp
RefSeq ORF 1443 bp
Locus ID 24185
UniProt ID P47196
Cytogenetics 6q32
Gene Summary inhibits JUN kinase activation and mediates inhibition of apoptosis [RGD, Feb 2006]

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