Grin2a (NM_008170) Mouse Tagged ORF Clone

CAT#: MG227135

Grin2a (tGFP-tagged) - Mouse glutamate receptor ionotropic NMDA2A (epsilon 1) (Grin2a), (10ug)

Product Images

Circular map for MG227135

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grin2a
• Synonyms: GluN2A; NMDAR2A; NR2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227135 representing NM_008170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGACTGGGCTACTGGACCTTGCTGGTATTGCCGGCCCTTCTGGTCTGGCACGGTCCGGCGCAGA
  ACGCGGCGGCGGAGAAGGGTACTCCAGCGCTGAACATTGCGGTGCTGCTGGGTCACAGCCACGACGTGAC
  AGAACGCGAACTTCGAAATCTGTGGGGCCCGGAGCAGGCAACCGGCTTGCCCCTGGATGTGAACGTGGTG
  GCGTTATTGATGAACCGCACTGACCCTAAGAGCCTCATCACGCATGTGTGCGACCTCATGTCCGGGGCGC
  GCATCCATGGCTTGGTGTTTGGAGATGATACGGACCAAGAGGCTGTGGCTCAGATGCTGGATTTTATCTC
  CTCACAGACTTTCATCCCCATCTTGGGCATTCATGGGGGTGCATCTATGATCATGGCTGACAAGGATCCG
  ACATCCACGTTCTTCCAGTTTGGAGCTTCCATCCAGCAGCAAGCCACGGTCATGCTGAAGATCATGCAGG
  ACTATGACTGGCATGTCTTCTCCTTGGTCACCACCATCTTCCCTGGCTACAGAGACTTCATCAGCTTCAT
  CAAGACAACAGTGGACAACAGCTTTGTGGGCTGGGATATGCAGAACGTGATCACACTGGACACCTCTTTT
  GAGGACGCCAAGACACAGGTCCAGCTGAAGAAGATCCACTCCTCTGTCATCCTGCTCTACTGCTCCAAGG
  ATGAGGCTGTCCTCATCCTGAGCGAGGCTCGCTCTCTTGGCCTCACCGGCTACGATTTCTTCTGGATTGT
  CCCCAGTTTGGTCTCCGGGAACACAGAGCTCATCCCCAAAGAGTTTCCATCGGGTCTCATTTCAGTCTCT
  TACGACGACTGGGACTACAGTCTGGAGGCAAGAGTGAGAGACGGTCTTGGGATCTTAACCACTGCCGCAT
  CTTCCATGTTGGAGAAATTCTCCTACATTCCCGAGGCCAAGGCCAGCTGCTACGGGCAGACAGAGAAGCC
  GGAGACCCCGCTACACACTCTGCACCAATTTATGGTCAATGTGACTTGGGATGGCAAGGACTTGTCCTTC
  ACTGAGGAAGGCTATCAGGTGCACCCCAGGCTTGTGGTGATCGTGCTGAATAAGGACCGGGAATGGGAAA
  AGGTGGGCAAGTGGGAGAATCAGACTCTGAGCCTGCGGCATGCTGTGTGGCCAAGGTATAAGTCCTTTTC
  TGACTGTGAGCCAGATGACAACCACCTCAGCATTGTCACCTTGGAGGAAGCCCCCTTCGTCATCGTAGAA
  GACATAGACCCACTGACTGAGACCTGCGTCAGGAACACGGTACCCTGTCGGAAGTTTGTCAAGATCAACA
  ATTCAACCAACGAAGGGATGAATGTGAAGAAGTGCTGCAAGGGGTTCTGCATCGACATCCTTAAGAAACT
  GTCCAGAACTGTAAAGTTCACCTATGACCTCTACCTGGTGACCAATGGGAAGCATGGGAAAAAGGTTAAC
  AATGTGTGGAATGGAATGATAGGCGAAGTGGTCTATCAACGAGCAGTTATGGCCGTGGGCTCCCTCACCA
  TCAATGAGGAGCGTTCAGAAGTGGTGGACTTCTCCGTGCCCTTTGTGGAGACAGGAATCAGTGTCATGGT
  CTCCAGGAGTAATGGCACTGTTTCCCCTTCTGCTTTCCTTGAACCCTTCAGCGCCTCTGTCTGGGTGATG
  ATGTTCGTGATGCTGCTCATTGTCTCTGCCATTGCTGTCTTCGTTTTTGAATACTTCAGTCCTGTTGGAT
  ACAACAGAAACTTAGCCAAAGGGAAAGCTCCCCATGGGCCTTCTTTTACCATTGGAAAAGCTATATGGCT
  CCTCTGGGGCCTGGTCTTCAACAATTCTGTGCCCGTCCAGAATCCTAAAGGCACAACCAGCAAGATAATG
  GTATCAGTGTGGGCCTTCTTTGCCGTCATCTTCCTTGCAAGTTACACAGCCAACCTGGCTGCCTTCATGA
  TCCAGGAGGAGTTTGTGGACCAAGTGACTGGCCTCAGTGACAAGAAGTTCCAGAGACCTCATGACTATTC
  TCCGCCTTTCCGATTTGGGACAGTACCCAATGGAAGTACAGAAAGGAATATTCGTAACAACTACCCCTAT
  ATGCACCAGTACATGACCAAATTCAACCAGAGGGGCGTAGAGGATGCCTTGGTCAGCTTGAAAACTGGGA
  AGTTGGACGCTTTCATCTATGACGCAGCTGTCTTGAACTACAAGGCCGGGAGGGATGAAGGCTGTAAACT
  GGTGACCATTGGGAGCGGGTACATCTTTGCCACCACAGGCTATGGAATTGCTCTGCAGAAGGGCTCACCC
  TGGAAGAGGCAGATTGACCTCGCTCTGCTCCAGTTTGTTGGTGACGGTGAGATGGAAGAGCTGGAGACAC
  TGTGGCTTACGGGCATCTGCCACAACGAGAAGAATGAGGTGATGAGCAGCCAGCTGGACATCGACAACAT
  GGCAGGAGTTTTCTACATGCTGGCTGCAGCTATGGCCCTCAGCCTCATCACCTTCATCTGGGAGCACCTC
  TTCTACTGGAAGCTGCGCTTCTGCTTCACAGGCGTGTGTTCTGACCGGCCCGGGCTGCTCTTCTCCATCA
  GCAGGGGCATCTACAGTTGCATCCATGGAGTGCACATTGAAGAAAAGAAGAAGTCTCCAGACTTCAATCT
  GACTGGGTCACAGAGCAACATGCTAAAGCTTCTCCGCTCAGCTAAAAACATCTCCAACATGTCCAACATG
  AACTCCTCGCGAATGGACTCACCCAAAAGAGCTGCTGACTTCATCCAAAGAGGCTCACTTATTGTGGACA
  TGGTTTCAGACAAGGGAAATTTGATATACTCAGATAACAGGTCCTTTCAAGGGAAGGACAGTATATTTGG
  AGAAAACATGAATGAACTGCAAACATTTGTGGCCAACAGGCACAAGGATAGTCTCAGTAACTATGTGTTT
  CAGGGACAGCATCCTCTCACTCTCAATGAGTCCAACCCCAACACAGTGGAGGTGGCTGTCAGCACTGAAT
  CCAAAGGGAACTCCCGACCCCGGCAGCTTTGGAAGAAATCCATGGAGTCTCTACGCCAGGATTCTCTAAA
  CCAGAACCCAGTCTCCCAGAGGGATGAGAAGACTGCAGAGAATAGGACCCACTCCCTAAAGAGTCCTAGG
  TATCTTCCAGAAGAGGTAGCCCATTCTGACATTTCTGAAACCTCAAGCCGGGCCACATGCCACAGGGAGC
  CAGATAATAATAAGAACCACAAGACCAAGGATAACTTCAAAAGGTCAATGGCCTCTAAATACCCCAAGGA
  CTGTAGTGAGGTTGAACGTACCTACGTGAAAACCAAAGCAAGTTCTCCCAGGGATAAGATCTACACCATC
  GATGGTGAGAAGGAGCCCAGCTTCCACTTAGATCCTCCACAGTTCATTGAAAACATAGTCTTGCCTGAGA
  ATGTGGACTTCCCAGATACCTACCAAGATCACAATGAGAATTTCCGCAAGGGGGACTCCACACTGCCCAT
  GAACAGGAACCCACTACACAATGAAGATGGGCTTCCCAACAATGACCAGTATAAACTCTATGCCAAGCAC
  TTTACCTTGAAAGACAAGGGTTCCCCACATAGTGAGGGCAGTGATCGATATCGGCAGAACTCCACGCATT
  GCAGAAGCTGCCTCTCAAACCTGCCCACCTACTCAGGCCACTTTACCATGAGATCTCCTTTCAAGTGTGA
  TGCCTGTCTGCGGATGGGGAACCTCTATGACATTGATGAAGACCAGATGCTTCAGGAGACAGGCAACCCA
  GCTACTCGTGAGGAGGCCTACCAGCAGGACTGGTCACAGAACAACGCCCTCCAGTTCCAGAAGAACAAGC
  TAAAGATTAATCGACAGCACTCCTATGATAACATTCTCGACAAACCCAGGGAGATAGACCTTAGCAGGCC
  CTCTCGTAGCATAAGCCTCAAGGACAGGGAAAGGCTACTGGAGGGCAACTTATACGGGAGCCTGTTCAGT
  GTCCCCTCAAGCAAACTCTTGGGGAACAAAAGCTCCCTTTTCCCCCAAGGTCTGGAGGACAGCAAGAGGA
  GCAAATCTCTCTTGCCAGACCATACCTCTGATAATCCTTTCCTCCACACGTACGGGGATGACCAACGCTT
  AGTTATTGGGAGATGTCCCTCGGACCCTTACAAACACTCATTGCCATCACAGGCAGTAAATGACAGCTAT
  CTTCGGTCATCCTTGAGGTCAACAGCATCATATTGCTCCAGGGACAGTCGGGGCCACAGTGATGTGTATA
  TTTCAGAGCATGTTATGCCTTATGCTGCAAATAAGAATAACATGTACTCTACCCCCAGGGTTTTAAATTC
  CTGCAGCAATAGACGTGTGTACAAGAAAATGCCTAGTATTGAATCTGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227135 representing NM_008170
  Red=Cloning site Green=Tags(s)
  
  MGRLGYWTLLVLPALLVWHGPAQNAAAEKGTPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVV
  ALLMNRTDPKSLITHVCDLMSGARIHGLVFGDDTDQEAVAQMLDFISSQTFIPILGIHGGASMIMADKDP
  TSTFFQFGASIQQQATVMLKIMQDYDWHVFSLVTTIFPGYRDFISFIKTTVDNSFVGWDMQNVITLDTSF
  EDAKTQVQLKKIHSSVILLYCSKDEAVLILSEARSLGLTGYDFFWIVPSLVSGNTELIPKEFPSGLISVS
  YDDWDYSLEARVRDGLGILTTAASSMLEKFSYIPEAKASCYGQTEKPETPLHTLHQFMVNVTWDGKDLSF
  TEEGYQVHPRLVVIVLNKDREWEKVGKWENQTLSLRHAVWPRYKSFSDCEPDDNHLSIVTLEEAPFVIVE
  DIDPLTETCVRNTVPCRKFVKINNSTNEGMNVKKCCKGFCIDILKKLSRTVKFTYDLYLVTNGKHGKKVN
  NVWNGMIGEVVYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLEPFSASVWVM
  MFVMLLIVSAIAVFVFEYFSPVGYNRNLAKGKAPHGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIM
  VSVWAFFAVIFLASYTANLAAFMIQEEFVDQVTGLSDKKFQRPHDYSPPFRFGTVPNGSTERNIRNNYPY
  MHQYMTKFNQRGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTIGSGYIFATTGYGIALQKGSP
  WKRQIDLALLQFVGDGEMEELETLWLTGICHNEKNEVMSSQLDIDNMAGVFYMLAAAMALSLITFIWEHL
  FYWKLRFCFTGVCSDRPGLLFSISRGIYSCIHGVHIEEKKKSPDFNLTGSQSNMLKLLRSAKNISNMSNM
  NSSRMDSPKRAADFIQRGSLIVDMVSDKGNLIYSDNRSFQGKDSIFGENMNELQTFVANRHKDSLSNYVF
  QGQHPLTLNESNPNTVEVAVSTESKGNSRPRQLWKKSMESLRQDSLNQNPVSQRDEKTAENRTHSLKSPR
  YLPEEVAHSDISETSSRATCHREPDNNKNHKTKDNFKRSMASKYPKDCSEVERTYVKTKASSPRDKIYTI
  DGEKEPSFHLDPPQFIENIVLPENVDFPDTYQDHNENFRKGDSTLPMNRNPLHNEDGLPNNDQYKLYAKH
  FTLKDKGSPHSEGSDRYRQNSTHCRSCLSNLPTYSGHFTMRSPFKCDACLRMGNLYDIDEDQMLQETGNP
  ATREEAYQQDWSQNNALQFQKNKLKINRQHSYDNILDKPREIDLSRPSRSISLKDRERLLEGNLYGSLFS
  VPSSKLLGNKSSLFPQGLEDSKRSKSLLPDHTSDNPFLHTYGDDQRLVIGRCPSDPYKHSLPSQAVNDSY
  LRSSLRSTASYCSRDSRGHSDVYISEHVMPYAANKNNMYSTPRVLNSCSNRRVYKKMPSIESDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008170
• ORF Size: 4392 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008170.4
• RefSeq Size: 4512 bp
• RefSeq ORF: 4395 bp
• Locus ID: 14811
• UniProt ID: P35436
• Cytogenetics: 16 5.28 cM
• Gene Summary: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium (PubMed:1374164). Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have higher sensitivity to glutamate and faster kinetics than channels formed by GRIN1 and GRIN2B (By similarity). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (PubMed:7816096, PubMed:8987814).[UniProtKB/Swiss-Prot Function]