POLD3 (NM_006591) Human 3' UTR Clone

CAT#: SC217193

3' UTR clone of polymerase (DNA-directed) delta 3 accessory subunit (POLD3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLD3
• Synonyms: P66; P68; PPP1R128
• ACCN: NM_006591
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC217193 3’UTR clone of NM_006591
  The sequence shown below is from the reference sequence of NM_006591. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCCATTACTGGCTTCTTCCAGAGGAAATAAACTGCCATCTCTGGTAGATCAGAGACTTGGAGTGGTCAA
  GGGAGAAGACCAAGAAATGTACTCCTCACTTACTATGTAAGTTCATCTAGATCTCCACCTCACCTGTAT
  CAAAAGACTGTTCTTTCATCCTGTGAGGTTTATACTATTTCTGGTTTTTAACCAAAAGGAAATCATCTG
  GAAGCAGGAGGCAAAAAGCTGTTACCTTCTAATGACATTTAAAAAGCACAGTCTTTGACCTGTCCAGGA
  GAAGGATTTACTCCAAAATTATACTGGAACAGTTTTCAGAATTCTCACTGAAGCCATTTAGTGGCTAAC
  CCACTGTGCTCCACTCACCCTATGCCCTGGTCCGCATATGGCACAGGAATTATTCCTTCTTGTTCCTCT
  GTATTCTAAGAATTCATGTGGGTGTTTCTTATCCTTACATTCTGCTGGATACGTTTACCCCTCTTGTCT
  TCCTGCAGTACCACACTTCTGTCCCCTAACCTCCTGAGGCTGTTCTCTGTAAGTCTTTTAAGTTTTGGT
  TGGACTAAAGGCTGAAGTGAAAATCTCCCTGTAATCCTCTTCCTCCATTATGCAGTACACGGACACCTG
  GCTACAGACCAGGACGTGGCTTGTGTTCTGTTCCTTTCACAAAAGCTCTCTGGGACCTTCACTTGCAAT
  TAGTGGTTAGGGAAAAGCCTCAGGCAGAACACAAATAGAAATTTAATGATGTGTTCAACTCCACCAGAA
  ATTACCTCGAGTCAGCATTGACGATATTGGAGGAGCTGCCGTGCTGCTGATACGGGGTGTGCTTTATGC
  TGCTCTTTGCGGTTTGTTGATCCCCTCCTCCCCCACTCTCAATACCTAGAGAGTGAAACCCGTACAATG
  AGATAAAGACTAAAAAGAGAAATCCCTTCCTATATACAGTGTGCTACATTTACAAAAAATTTCTCCTTA
  AGAAAACAGAATATTCAAAAACAGCACTTTTCCAGGAAGTTAGTGAGGAAGATAAGGCACACATTTATT
  TATAAATGGATGTTGCTCCTTTGTATTTTTAGCTTCCCTATTTGCTTAAAAGTACAGGCTCCCTAAGAG
  AGGATGGAGAGGGAGAGACTGAATTGTTAGTAGGTCTAAACATCAAAGAAAACATTTTTATTAGTTACT
  TATGGAAAATCATCTATTACAATGATAACCTTCAAGTGACTTCCATTATGGCTGGACAGGCGGTGAGCT
  CAGTGGATTGCAGTGGTGTGCTGGTGATTTTGCACAGTGAGCTCTGCGGAAGGGGTCAGGCTCAACTGC
  TGATGTGTCTCACACGTAGCAGACAAGGGGTGTCTGACTGGCTTCTTTTGCCTCAAGATGGTGTATGTC
  GTAAGTGATGCAATCAGCTGTCTGCTTTTTAAGGTTGGGATTGTGCTGACTTTGGGATTAACATGAGCT
  TCTTTAGCAACCAAGCATGAACTTGATTAAGACCAGAAGTTTGGGAGATGAGTCCTGGCATTATGTCTA
  GGACTAAAGCAGTGGCTTTGTATAGCAAGCTGAGTAAAGGTTGACATATTCCAAAACCCTTCTTTTTAA
  AAGGAAAAAGGATGGAGAGAAGGATGGAAAGCCTGGACTTAAACCTTTAGAAAAAACTTCTGGAGAGAA
  ATCCCTTTTAAACAGTTACTTTTGTCATTGCCTCTGGTCATTTGTCTAAATAGGAATGGAAAATTAAAA
  GAAAAGCAACAATCCAATCTTTTTTCTAAAAATTATGCTGGGGTCTCGACTAAAACTGAATTTGAATTG
  GAAAATTCTGGTGTTGGTTGGAGTTCCATCTTGCAAGGGATAATACAAATCCTATGATCTCTATGCCCA
  ATATGCTGCCTCAACTCTGAGCTGTCTGCAAGGCTTAGTAAGTATTGAGTGGTGTTTTTTTTTTCTTTT
  TATACAATACCATGTTAACCACATGAGTTAAATAAATTTGAGAAGTTGTTTTAAAACAGTGCTTCAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006591.3
• Summary: This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]
• Locus ID: 10714
• MW: 76