ORP150 (HYOU1) (NM_006389) Human 3' UTR Clone

CAT#: SC214544

3' UTR clone of hypoxia up-regulated 1 (HYOU1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ORP150
• Synonyms: GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150
• ACCN: NM_006389
• Insert Size: 1435 bp
• Sequence Data:
  Insert Sequence

  >SC214544 3’UTR clone of NM_006389
  The sequence shown below is from the reference sequence of NM_006389. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGCGGCCTTTGAAGAACGACGAACTATAACCCCCACCTCTGTTTTCCCCATTCATCTCCACCCCCTTC
  CCCCACCACTTCTATTTATTTAACATCGAGGGTTGGGGGAGGGGTTGGTCCTGCCCTCGGCTGGAGTTC
  CTTTCTCACCCCTGTGATTTGGAGGTGTGGAGAAGGGGAAGGGAGGGACAGCTCACTGGTTCCTTCTGC
  AGTACCTCTGTGGTTAAAAATGGAAACTGTTCTCCTCCCCAGCCCCACTCCCTGTTCCCTACCCATATA
  GGCCCTAAATTTGGGAAAAATCACTATTAATTTCTGAATCCTTTGCCTGTGGGTAGGAAGAGAATGGCT
  GCCAGTGGCTGATGGGTCCCGGTGATGGGAAGGGTATCAGGTTGCTGGGGAGTTTCCACTCTTCTCTGG
  TGATTGTTCCTTCCCTCCCTTCCTCTCCCACCATGCGATGAGCATCCTTTCAGGCCAGTGTCTGCAGAG
  CCTCAGTTACCAGGTTTGGTTTCTGAGTGCCTATCTGTGCTCTTTCCTCCCTCTGCGGGCTTCTCTTGC
  TCTGAGCCTCCCTTCCCCATTCCCATCCAGCTCCTTTCCCCCTGGGTTTCCTTGGCTTCCTGCAGCAAA
  TTGGGCAGTTCTCTGCCCCTTGCCTAAAAGCCTGTACCTCTGGATTGGCGGAAGTAAATCTGGAAGGAT
  TCTCACTCGTATTTCCCACCCCTAGTGGCCAGAGGAGGGAGGGGCACAGTGAAGAAGGGAGCCCACCAC
  CTCTCCGAAGAGGAAAGCCACGTAGAGTGGTTGGCATGGGGTGCCAGCATCGTGCAAGCTCTGTCATAA
  TCTGCATCTTCCCAGCAGCCTGGTACCCCAGGTTCCTGTAACTCCCTGCCTCCTCCTCTCTTCTGCTGT
  TCTGCTCCTCCCAGACAGAGCCTTTCCCTCACCCCCTGACCCCCTGGGCTGACCAAAATGTGCTTTCTA
  CTGTGAGTCCCTATCCCAAGATCCTGGGGAAAGGAGAGACCATGGTGTGAATGTAGAGATGCCACCTCC
  CTCTCTCTGAGGCAGGCCTGTGGATGAAGGAGGAGGGTCAGGGCTGGCCTTCCTCTGTGCATCACTCTG
  CTAGGTTGGGGGCCCCCGACCCACCATACCTACGCCTAGGGAGCCCGTCCTCCAGTATTCCGTCTGTAG
  CAGGAGCTAGGGCTGCTGCCTCAGCTCCAAGACAAGAATGAACCTGGCTGTGTCAGTCATTTTGTCTTT
  TCCTTTTTTTTTTTTTTTTGCCACATTGGCAGAGATGGGACCTAAGGGTCCCACCCCTCACCCCACCCC
  CACCTCTTCTGTATGTTTGAATTCTTTCAGTAGCTGTTGATGCTGGTTGGACAGGTTTGAGTCAAATTG
  TACTTTGCTCCATTGTTAATTGAGAAACTGTTTCAATAAAATATTCTTTTCTACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006389.5
• Summary: The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
• Locus ID: 10525
• MW: 53.3