RASSF1 (NM_007182) Human 3' UTR Clone

CAT#: SC210114

3' UTR clone of Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) transcript variant A for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RASSF1
• Synonyms: 123F2; NORE2A; RASSF1A; RDA32; REH3P21
• ACCN: NM_007182
• Insert Size: 823 bp
• Sequence Data:
  Insert Sequence

  >SC210114 3’UTR clone of NM_007182
  The sequence shown below is from the reference sequence of NM_007182. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGGCCCTGCACGCCTGCCCCCTTGGGTGACCTCTTGTACCCCCAGGTGGAAGGCAGACAGCAGGCAGC
  GCCAAGTGCGTGCCGTGTGAGTGTGACAGGGCCAGTGGGGCCTGTGGAATGAGTGTGCATGGAGGCCCT
  CCTGTGCTGGGGGAATGAGCCCAGAGAACAGCGAAGTAGCTTGCTCCCTGTGTCCACCTGTGGGTGTAG
  CCAGGTATGGCTCTGCACCCCTCTGCCCTCATTACTGGGCCTTAGTGGGCCAGGGCTGCCCTGAGAAGC
  TGCTCCAGGCCTGCAGCAGGAGTGGTGCAGACAGAAGTCTCCTCAATTTTTGTCTCAGAAGTGAAAATC
  TTGGAGACCCTGCAAACAGAACAGGGTCATGTTTGCAGGGGTGACGGCCCTCATCTATGAGGAAAGGTT
  TTGGATCTTGAATGTGGTCTCAGGATATCCTTATCAGAGCTAAGGGTGGGTGCTCAGAATAAGGCAGGC
  ATTGAGGAAGAGTCTTGGTTTCTCTCTACAGTGCCAACTCCTCACACACCCTGAGGTCAGGGAGTGCTG
  GCTCACAGTACAGCATGTGCCTTAATGCTTCATATGAGGAGGATGTCCCTGGGCCAGGGTCTGTGTGAA
  TGTGGGCACTGGCCCAGGTTCATACCTTATTTGCTAATCAAAGCCAGGGTCTCTCCCTCAGGTGTTTTT
  TATGAAGTGCGTGAATGTATGTAATGTGTGGTGGCCTCAGCTGAATGCCTCCTGTGGGGAAAGGGGTTG
  GGGTGACAGTCATCATCAGGGCCTGGGGCCTGAGAGAATTGGCTCAATAAAGATTTCAAGATCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_007182.5
• Summary: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
• Locus ID: 11186
• MW: 29.1