HLCS (NM_001242784) Human Untagged Clone

CAT#: SC330013

HLCS (untagged) - Homo sapiens holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLCS
• Synonyms: HCS
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC330013 representing NM_001242784.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCACTTGCAGGAC
  TCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAA
  CCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTT
  GGTGAAGAACCCAAACAAAGGAGAGGCAGTGCCTCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGA
  GGGGGCCCCGTTGAGCATTATCACCTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGC
  ACCATTGAGTCAGTCAAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGT
  TTGGAGAGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAGGCA
  CCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCACGAGGTCCGGTCT
  GTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTGCTGGAGGACAGTGCTCTCAGA
  GACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCTACCAGGGAGTCCATTCCCGAAGACCTGTAC
  CAGAAGTTCATGGCCTATCTTTCTCAGGGAGGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGT
  GGCTTTCAGGTGACAAGCAAGGGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGAC
  CAGAGCGAGGTGAAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGC
  CCCGGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCTTTTGGA
  ACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGCTCCAACATAGTGCAA
  ACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGATACGAAGTCCTTAGAGAGATTCTG
  ACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTTCCTGCCTTAACTCCTCTTTACTTGCTGTCAGCT
  GCGGAGGAAATCAGGGATCCTCTTATGCAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAA
  TCCGGCCAGCTCTCTCTTAGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATA
  CCTGTGGTGACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG
  CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGTCTCCTGGAT
  GGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCCCGGCAGACCGAGGGCAAA
  GGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCTCTTTCTACTCTGCTCATCTCCATTCCA
  CTGAGATCCCAGCTGGGACAGAGGATCCCGTTTGTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCA
  GTGAGGTCCATTCCCGAGTATCAGGATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGT
  GACCTCATGAAGATCGGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATT
  GGCTGTGGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATACAAT
  AAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTCGTGACTGTGCTG
  GAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTTCCCCTTTATTACCGATACTGG
  GTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAGGGACCAAAGGTGTCCATCGTTGGCCTGGAC
  GATTCTGGCTTCCTCCAGGTTCACCAGGAGGGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCC
  TTCGACATGCTGAGAAACCTCATCCTCCCCAAACGGCGGTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001242784
• Insert Size: 2181 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242784.1
• RefSeq Size: 6953 bp
• RefSeq ORF: 2181 bp
• Locus ID: 3141
• UniProt ID: P50747
• Cytogenetics: 21q22.13
• Protein Pathways: Biotin metabolism, Metabolic pathways
• MW: 80.8 kDa
• Gene Summary: This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.