SHARP1 (BHLHE41) (NM_030762) Human Untagged Clone

CAT#: SC310341

BHLHE41 (untagged)-Human basic helix-loop-helix family, member e41 (BHLHE41)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHARP1
• Synonyms: BHLHB3; DEC2; FNSS1; hDEC2; SHARP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_030762 edited
  CTGGTCTGGCCGGCGACGCGCGTGCCCTGTGGCCAAACACTGCCTGGAGTGAGAGCAAAC
  TACCAGCGCAGTGGGGCCGGCGCGAGTGTGCGTGTGTGTGCGTGTGTGTGTGCGAGCGCG
  GTGGAGGGGGGGGACCAACTGCTTCACACTTTCAACACTGCACTGAAGAGGGAGAGCGAG
  AGAGAGACTGGAGACGCACAGATCCCCCCAAGGTCTCCCAAGCCTACCGTCCCACAGATT
  ATTGTACAGAGCCCCAAAAATCGAAACAGAGGAAACGAACAGCAGTTGAACATGGACGAA
  GGAATTCCTCATTTGCAAGAGAGACAGTTACTGGAACATAGAGATTTTATAGGACTGGAC
  TATTCCTCTTTGTATATGTGTAAACCCAAAAGGAGCATGAAACGAGACGACACCAAGGAT
  ACCTACAAATTACCGCACAGATTAATAGAAAAGAAAAGAAGAGACCGAATTAATGAATGC
  ATTGCTCAGCTGAAAGATTTACTGCCTGAACATCTGAAATTGACAACTCTGGGACATCTG
  GAGAAAGCTGTAGTCTTGGAATTAACTTTGAAACACTTAAAAGCTTTAACCGCCTTAACC
  GAGCAACAGCATCAGAAGATAATTGCTTTACAGAATGGGGAGCGATCTCTGAAATCGCCC
  ATTCAGTCCGACTTGGATGCGTTCCACTCGGGATTTCAAACATGCGCCAAAGAAGTCTTG
  CAATACCTCTCCCGGTTTGAGAGCTGGACACCCAGGGAGCCGCGGTGTGTCCAGCTGATC
  AACCACTTGCACGCCGTGGCCACCCAGTTCTTGCCCACCCCGCAGCTGTTGACTCAACAG
  GTCCCTCTGAGCAAAGGCACCGGCGCTCCCTCGGCCGCCGGGTCCGCGGCCGCCCCCTGC
  CTGGAGCGCGCGGGGCAGAAGCTGGAGCCCCTCGCCTACTGCGTGCCCGTCATCCAGCGG
  ACTCAGCCCAGCGCCGAGCTCGCCGCCGAGAACGACACGGACACCGACAGCGGCTACGGC
  GGCGAAGCCGAGGCCCGGCCGGACCGCGAGAAAGGCAAAGGCGCGGGGGCGAGCCGCGTC
  ACCATCAAGCAGGAGCCTCCCGGGGAGGACTCGCCGGCGCCCAAGAGGATGAAGCTGGAT
  TCCCGCGGCGGCGGCAGCGGCGGCGGCCCGGGGGGCGGCGCGGTGGCGGCGGCAGCCGCG
  CTTCTGGGGCCCGACCCTGCCGCCGCGGCCGCGCTGCTGAGACCCGACGCCGCCCTGCTC
  AGCTCGCTGGTGGCGTTCGGCGGAGGCGGAGGCGCGCCCTTCCCGCAGCCCGCGGCCGCC
  GCGGCCCCCTTCTGCCTGCCCTTCTGCTTCCTCTCGCCTTCTGCAGCTGCCGCCTACGTG
  CAGCCCTTCCTGGACAAGAGCGGCCTGGAGAAGTATCTGTACCCGGCGGCGGCTGCCGCC
  CCGTTCCCGCTGCTATACCCCGGCATCCCCGCCCCGGCGGCAGCCGCGGCAGCCGCCGCC
  GCCGCTGCCGCCGCCGCCGCCGCGTTCCCCTGCCTGTCCTCGGTGTTGTCGCCCCCTCCC
  GAGAAGGCGGGCGCCGCCGCCGCGACCCTCCTGCCGCACGAGGTGGCGCCCCTTGGGGCG
  CCGCACCCCCAGCACCCGCACGGCCGCACCCACCTGCCCTTCGCCGGGCCCCGCGAGCCG
  GGGAACCCGGAGAGCTCTGCTCAGGAAGATCCCTCGCAGCCAGGAAAGGAAGCTCCCTGA
  ATCCTTGCGTCCCGAAGGACGGAGGTTCAAGCAGAGTGAGAAGTTAAAATACCCTTAAGG
  AGGTTCAAGCAGAGTGAGAAGTTAAAATACCCTTAAGGTCTTTAAGGGAGGAAGTGTAAT
  AGATGCACGACAGGCATAAACAAGAACAACAAAACAGGTGTTATGTGTACATTCGGAGTT
  CCTGTTTTGCTCATCCCGCACCACCCCACCCTCCACACACTAACATCCCTTTCTTCCCCC
  CACCAGCTGTAAAAGATCCTATGCGAAAGACACTGGCTCTTTTTTTTAATCCCCCAAATA
  AATTTTGCCCCCTTTTAGGCCATGTTCCATTATCTCTTAAAATTGGAACCTAATTCGAGA
  GGAAGTAAGAAGGGTCTGTTCTGTGGCTGAGCTAGGTGAACCCCGGGGTAGGGGAAAGAT
  GTTAACACCTTTGACGTCTTTGGAGTTGACATGGAACAGCAGGTAGTTGTTATGTAGAGC
  TAGTTCTCAAAGCTGCCCTGCCTGTTTTAGGAGGCGTTCCACAAACAGATTGAGGCTCTT
  TTAGAATTGAATTTACTCTTCAGTATTTTCTAATGTTCAGCTTTCTAAAAGGCATATATT
  TTTCAAAGAAGTGAGGATGCAGTTTCTCACGTTGCAACCTATTCTGAAGTGGTTTAAATG
  GTATCTCTTAGTAACTTGCACTCGTTAAAGAAACACGGAGCTGGGCCATCGTCAGAACTA
  AGTCAGGGAAGGAGATGGATGAGAAGGCCAGAATCATTCCTAGTACATTTGCTAACACTT
  TATTGAGAAATTGACCATGAATTAATGGACTCATCTTAATTTCTTCTAAGTCCATATATA
  GATAGATATCTATCTGTACAGATTTCTATTTATCCATAGATAGGTATCTATACATACACA
  TCTCAAGTGCATCTATTCCCACTCTCATTAATCCATCATGTTCCTAAATTTTTGTAATCT
  TACTGTAAAAAAAAGTGCACTGAACTTCAAAACAAAACAAAAAACAACAACAACAAAAAA
  CAAGTCCAAACTGATATATCCTATATTCTGTTAAAATTCAAAAGTGAACGAAAGCATTTA
  ACTGGCCAGTTTTGATTGCAAATGCTGTAAAGATATAGAATGAAGTCCTGTGAGGCCTTC
  CTATCTCCAAGTCTATGTATTTTCTGGAGACCAAACCAGATACCAGATAATCACAAAGAA
  AGCTTTTTTAATAAGGCTTAAACCAAGACCTTGTCTAGATATTTTTAGTTTGTTGCCAAG
  GTAGCACTGTGAGAAATCTCACTTGGATGTTATGTAAGGGGTGAGACACAACAGTCTGAC
  TATGAGTGAGGAAAATATCTGGGTCTTTTCGTCAGTTTGGTGCATTTGCTGCTGCTGTTG
  CTACTGTTTGCCTCAAACGCTGTGTTTAAACAACGTTAAACTCTTAGCCTACAAGGTGGC
  TCTTATGTACATAGTTGTTAATACATCCAATTAATGATGTCTGACATGCTATTTTTGTAG
  GGAGAAAATATGTGCTAATGATATTTTGAGTTAAAATATCTTTTGGGGAGGATTTGCTGA
  AAAGTTGCACTTTTGTTACAATGCTTATGCTTGGTACAAGCTTATGCTGTCTTAAATTAT
  TTTAAAAAAATAAATACTGTCTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_030762
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030762.1, NP_110389.1
• RefSeq Size: 3641 bp
• RefSeq ORF: 1449 bp
• Locus ID: 79365
• UniProt ID: Q9C0J9
• Cytogenetics: 12p12.1
• Domains: HLH, ORANGE
• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]