BTD (NM_000060) Human Untagged Clone

CAT#: SC310282

BTD (untagged)-Human biotinidase (BTD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BTD
• Synonyms: biotinidase
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000060 edited
  ATGGCGCATGCGCATATTCAGGGCGGAAGGCGCGCTAAGAGCAGATTTGTGGTCTGCATT
  ATGTCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTG
  GGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCT
  GCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAA
  GAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGTGATGACTGCA
  GCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAACTTT
  ACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCAGGTGG
  AACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGTTGT
  ATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCAT
  AGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTC
  AGCAATAATGGAACCCTTGTTGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCA
  TTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGGTTT
  GGCATCTTCACATGCTTTGATATATTGTTCTTTGACCCTGCCATCAGAGTCCTCAGAGAC
  TACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAACCAGCTCCCACTCTTGGCA
  GCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTGGCAGCTAAT
  GTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTT
  TGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAAT
  CCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAG
  TTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGATGCTCAGGAAGTCCACTGT
  GATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATTTCACTCTGAGATGATGTAT
  GACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTATCTCCACGTCTGTTCCAAT
  GGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAAGAGCTGTATGCCCTG
  GGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGTGCCCTG
  GTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGAAATCACAGAGGCCACGGGG
  ATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTG
  ACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTC
  CTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGGCTCTCTATGGGCGCTTGTAT
  GAGAGGGACTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000060 unedited
  CCCACAGGGACACAACTATACAGGGCGGCCGCGACCCGCACGACAAACAGGTAGAGCCGA
  CCCCGAGCCTCCGCCCCGCCCCCGAGACGGCCAGCCGGAGCGAATTCGGGGCTGTAAAGG
  GAGAATGGCGCATGCGCAAATTCAGGGCGGAAGGCGCGCTAAGAGCAGACCCGTGGCCCG
  CAACATGCCTGGAGCCAGAAGCAAGCCCGCTCCCCTCCTCCGCGGCTGTCACGCGGTCGC
  CCTGGGAGCCCACACCGGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGT
  GGCTGCCGTGTATGAGCATCCATCCATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCG
  CCAAGAGGCCTTGGAGCTCATGAACCAGAACCTTGACATCTATGAACAGCAAGCGATGAC
  TGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGATGGCATTCATGGATTCAA
  CCTTACAAGAACATCCATCTTATCCATTTTTGGACTTCATGCCGTCTCCCCAGGTGGTCA
  GGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGA
  GTTGTATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTT
  GTCATAGCAGTGACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCG
  TGTTCAGCAATAATGGAACCCTTGTTGACCGCTACCGTAACACAACCTCTACTTTGAGCA
  GCATTCGATGTTCCTCTTAAAGTGGATCTCATCACCTTTGATACCCCCTTTGCTGGCAGT
  TTGGCATCTTCACATGCTTTGATAATATTGTTCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000060 unedited
  CATCATACGTTCTGCGCTATGTCCCCACCCAGTTCTGGGGATGACAGGAAGTGGCATACA
  CACCCCTCTGGAGTCCTTTTGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAAT
  TGCCCAGGTGGCCAAAAATCCAGTGGGTCTCATTGGTGCAGAGAATGCAACAGGTGAAAC
  GGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCAGGTGATCCGTACTGTGAGAAGGA
  TGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAATGCTCCTCCCACATT
  TCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAAGGCTA
  TCTCCACGTCTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATC
  CAAAGAGCTGTATGCCCTGGGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTA
  CATCCAAGTGTGTGCCCTGGTCAGGTGTGGGGGTCTTGGCTTCGACACCTGCGGACAGGA
  AATCACAGAGGCCACGGGGATATTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTA
  TATCTTTCCTTTGTTTCTGACCTCAGGGATGACCCTAGAAGTCCCTGACCAGCTTGGCTG
  GGAGAATGACCACTATTTTCCTGAGGAAAAGTAGGCTGTCCTCTGGGCTGGTGACGGCGG
  CTCTCTATGGGCGCTTGTATGAGAGGGACTAGGAAAAGTGTGTGGTCTGTGGGGCGGACT
  CTGGCCATCATGTTGACAGCCTTGCACTTCCACAGGCTACAAGCCCTGGGACCATCTTTC
  TGCCTTAAGGCAGGAGCCCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000060
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000060.2, NP_000051.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1632 bp
• Locus ID: 686
• UniProt ID: P43251
• Cytogenetics: 3p25.1
• Domains: CN_hydrolase
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Biotin metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1.