PER3 (NM_016831) Human Untagged Clone

CAT#: SC309952

PER3 (untagged)-Human period homolog 3 (Drosophila) (PER3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PER3
• Synonyms: FASPS3; GIG13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309952 representing NM_016831.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCCGCGGGGAAGCTCCTGGCCCCGGGAGACGGGGGGCTAAGGACGAGGCCCTGGGCGAAGAATCG
  GGGGAGCGGTGGAGCCCCGAGTTCCATCTGCAGAGGAAATTGGCGGACAGCAGCCACAGTGAACAGCAA
  GATCGAAACAGAGTTTCTGAAGAACTTATCATGGTTGTCCAAGAAATGAAAAAATACTTCCCCTCGGAG
  AGACGCAATAAACCAAGCACTCTAGATGCCCTCAACTATGCTCTCCGCTGTGTCCACAGCGTTCAAGCA
  AACAGTGAGTTTTTCCAGATTCTCAGTCAGAATGGAGCACCTCAGGCAGATGTGAGCATGTACAGTCTT
  GAGGAGCTGGCCACTATCGCTTCAGAACACACTTCCAAAAACACAGATACCTTTGTGGCAGTATTTTCA
  TTTCTGTCTGGAAGGTTAGTGCACATTTCTGAACAGGCTGCTTTGATCCTGAATCGTAAGAAAGATGTC
  CTGGCGTCTTCTCACTTTGTTGACCTGCTTGCACCTCAAGACATGAGGGTATTCTACGCGCACACTGCC
  AGAGCTCAGCTTCCTTTCTGGAACAACTGGACCCAAAGAGCTGCACGGTATGAATGTGCTCCGGTGAAA
  CCTTTTTTCTGCAGGATCCGTGGAGGTGAAGACAGAAAGCAAGAGAAGTGTCACTCCCCATTCCGGATC
  ATCCCCTATCTGATTCATGTACATCACCCTGCCCAGCCAGAATTGGAATCGGAACCTTGCTGTCTCACT
  GTGGTTGAAAAGATTCACTCTGGTTATGAAGCTCCTCGGATCCCAGTGAATAAAAGAATCTTCACCACC
  ACACACACCCCAGGGTGTGTTTTTCTTGAAGTAGATGAAAAAGCAGTGCCTTTGCTGGGTTACCTACCT
  CAGGACCTGATTGGAACATCGATCCTAAGCTACCTGCACCCTGAAGATCGTTCTCTGATGGTTGCCATA
  CACCAAAAAGTTTTGAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGATTTTGTACTCAA
  AACGGAGACTACATCATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCGGAAGATTTCT
  TTCATCATTGGTCGGCATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACCAAAATTAAA
  AAGATGAACGATAATGACAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCTTACAGCCA
  GTTCACGTGAGCGTGTCCAGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCTTGTCAGC
  ATCGCCTCCTCCAGTGAGGCCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACCTTGCAG
  CAGGTCTATGCCAGTGTGAACAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGACCAAA
  TCATCATTCAAGCCAGTGACGGGGACACGCACAGAACCGAATGGTGGTGGTGAATGTAAGACCTTTACT
  TCCTTCCACCAAACACTGAAAAACAATAGTGTGTACACTGAGCCCTGTGAGGATTTGAGGAACGATGAG
  CACAGCCCATCCTATCAACAGATCAACTGTATCGACAGTGTCATCAGATACCTGAAGAGCTACAACATT
  CCAGCTTTGAAAAGAAAGTGTATCTCCTGTACAAATACAACTTCTTCCTCCTCAGAAGAAGACAAACAG
  AACCACAAGGCAGATGATGTCCAAGCCTTACAAGCTGGTTTGCAAATCCCAGCCATACCTAAATCAGAA
  ATGCCAACAAATGGACGGTCCATAGACACAGGAGGAGGAGCTCCACAGATCCTGTCCACGGCGATGCTG
  AGCTTGGGGTCGGGCATAAGCCAATGCGGTTACAGCAGCACCATTGTCCATGTCCCACCCCCAGAGACA
  GCCAGGGATGCTACCCTCTTCTGTGAGCCCTGGACCCTGAACATGCAGCCAGCCCCTTTGACCTCGGAA
  GAATTTAAACACGTGGGGCTCACAGCGGCTGTTCTGTCAGCGCACACCCAGAAGGAAGAGCAGAATTAT
  GTTGATAAATTCCGAGAAAAGATCCTGTCATCACCCTACAGCTCCTATCTTCAGCAAGAAAGCAGGAGC
  AAAGCTAAATATTCATATTTTCAAGGAGATTCTACTTCCAAGCAGACGCGGTCGGCCGGCTGCAGGAAA
  GGGAAGCACAAGCGGAAGAAGCTGCCGGAGCCGCCAGACAGCAGCAGCTCGAACACCGGCTCTGGTCCC
  CGCAGGGGAGCGCATCAGAACGCACAGCCCTGCTGCCCCTCCGCGGCCTCCTCTCCGCACACCTCGAGC
  CCGACCTTCCCACCTGCCGCCATGGTGCCCAGCCAGGCCCCTTACCTCGTCCCAGCTTTTCCCCTCCCA
  GCCGCGACCTCACCCGGAAGAGAATACGCAGCCCCCGGAACTGCACCGGAAGGCCTGCATGGGCTGCCC
  TTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCTTTTCCTTACTTGGATACTTTTATGACCGTTTTC
  CTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCATCGTTTTTGCCATGTCCATTCCTGGGGGCGACA
  GCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCAATGAGTCCAACTCTGGACCCACCCCCTTCAGTC
  ACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCACAAAGCGAGGGGCACCCGTTCATTACTTCGAGA
  AGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAGAGATGCCCAGACCCTCTGAATCTCCAGATCAG
  ATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGTTACAGGCAACAATGGCAGTGAGAGCAGTCCT
  GCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGGGAGAATCCATCCCATCCTACTGCCAGCGCT
  CTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCG
  CCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCACACTGTCCATGGGATTGCCTCCCAGCAGGACT
  CCATCCCATCCTACTGCCACTGTTCTGTCCACGGGGTCACCTCCCAGCGAATCCCCATCCAGAACTGGT
  TCAGCAGCATCAGGAAGCAGCGACAGCAGTATATACCTTACTAGTAGTGTTTATTCTTCTAAAATCTCC
  CAAAATGGGCAGCAATCTCAGGACGTACAGAAAAAAGAAACATTTCCTAATGTCGCCGAAGAGCCCATC
  TGGAGAATGATACGGCAGACACCTGAGCGCATTCTCATGACATACCAGGTACCTGAGAGGGTTAAAGAA
  GTTGTACTAAAAGAAGACCTGGAAAAGCTAGAAAGTATGAGGCAGCAGCAGCCCCAGTTTTCTCATGGG
  CAAAAGGAGGAGCTGGCTAAGGTGTATAATTGGATTCAAAGCCAGACTGTCACTCAAGAAATCGACATT
  CAAGCCTGTGTCACTTGTGAAAATGAAGATTCAGCTGATGGTGCGGCCACATCCTGTGGTCAGGTTCTG
  GTAGAAGACAGCTGTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016831
• Insert Size: 3606 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016831.2
• RefSeq Size: 6309 bp
• RefSeq ORF: 3606 bp
• Locus ID: 8863
• UniProt ID: P56645
• Cytogenetics: 1p36.23
• Domains: PAS
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 131.9 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (4) uses alternate in-frame splice junctions at three exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.