PER2 (NM_022817) Human Untagged Clone

CAT#: SC309597

PER2 (untagged)-Human period homolog 2 (Drosophila) (PER2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PER2
• Synonyms: FASPS; FASPS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309597 representing NM_022817.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATGGATACGCGGAATTTCCGCCCAGCCCCAGTAACCCCACCAAGGAGCCCGTGGAGCCCCAGCCC
  AGCCAGGTCCCACTGCAGGAAGATGTGGACATGAGCAGTGGCTCCAGTGGACATGAGACCAACGAAAAC
  TGCTCCACGGGGCGGGACTCGCAGGGCAGTGACTGTGACGACAGTGGGAAGGAGCTGGGGATGCTGGTG
  GAGCCACCGGATGCCCGCCAGAGTCCAGATACCTTTAGCCTGATGATGGCAAAATCTGAACACAACCCA
  TCTACAAGTGGCTGCAGTAGCGACCAGTCTTCGAAAGTGGACACACACAAAGAACTGATAAAAACACTA
  AAGGAGCTGAAGGTCCACCTCCCTGCAGACAAGAAGGCCAAGGGCAAGGCCAGTACGCTGGCCACCTTG
  AAGTACGCCCTCAGGAGCGTGAAGCAGGTGAAAGCCAATGAAGAGTATTACCAGCTGCTGATGTCCAGC
  GAGGGTCACCCCTGTGGAGCAGACGTGCCCTCCTACACCGTGGAGGAGATGGAGAGCGTTACCTCTGAG
  CACATTGTGAAGAATGCCGATATGTTTGCGGTGGCCGTGTCCCTGGTGTCTGGGAAGATCCTGTACATC
  TCTGACCAGGTTGCATCCATATTTCACTGTAAAAGAGATGCCTTCAGCGATGCCAAGTTTGTGGAGTTC
  CTGGCGCCTCACGATGTGGGCGTGTTCCACAGTTTCACCTCCCCGTACAAGCTTCCCTTGTGGAGCATG
  TGCAGTGGAGCAGATTCTTTTACTCAAGAATGCATGGAGGAGAAATCTTTCTTTTGCCGTGTCAGTGTC
  CGGAAAAGCCACGAGAATGAAATCCGCTACCACCCCTTCCGCATGACGCCCTACCTGGTCAAGGTGCGG
  GACCAACAAGGTGCTGAGAGTCAGCTTTGCTGCCTTCTGCTGGCAGAGAGAGTGCACTCTGGTTATGAA
  GCCCCTAGAATTCCTCCTGAAAAGAGAATTTTTACAACCACCCATACACCAAATTGTTTGTTCCAGGAT
  GTGGATGAAAGGGCGGTCCCTCTCCTGGGCTACCTACCTCAGGACCTGATTGAAACCCCAGTGCTCGTG
  CAGCTCCACCCTAGTGACAGGCCCTTGATGCTGGCCATCCACAAAAAGATCCTGCAGTCAGGCGGGCAG
  CCTTTCGACTATTCTCCCATTCGGTTTCGCGCCCGGAACGGAGAGTACATCACGTTGGACACCAGCTGG
  TCCAGCTTCATCAACCCATGGAGCAGGAAAATCTCCTTCATCATTGGGAGGCACAAAGTCAGGGTGGGC
  CCTTTGAATGAGGACGTGTTTGCAGCCCACCCCTGCACAGAGGAGAAGGCCCTGCACCCCAGCATTCAG
  GAGCTCACAGAGCAGATCCACCGGCTCCTGCTGCAGCCCGTCCCCCACAGCGGCTCCAGTGGCTACGGG
  AGTCTGGGCAGCAACGGGTCCCACGAGCACCTTATGAGCCAGACCTCCTCCAGCGACAGCAACGGCCAT
  GAGGACTCACGCCGGAGGAGAGCCGAAATTTGTAAAAATGGTAACAAGACCAAAAATAGAAGTCATTAT
  TCTCATGAATCTGGAGAACAAAAGAAAAAATCCGTTACAGAAATGCAAACTAATCCCCCAGCTGAGAAG
  AAAGCTGTCCCTGCCATGGAAAAGGACAGCCTGGGGGTCAGCTTCCCCGAGGAGTTGGCCTGCAAGAAC
  CAGCCCACCTGCTCCTACCAGCAGATCAGCTGCTTGGACAGCGTCATCAGGTACTTGGAGAGCTGCAAT
  GAGGCTGCCACCCTGAAGAGGAAATGCGAGTTCCCAGCAAACGTCCCAGCGCTAAGGTCCAGTGATAAG
  CGGAAGGCCACAGTCAGCCCAGGGCCACACGCTGGAGAGGCAGAGCCGCCCTCCAGGGTGAACAGCCGC
  ACGGGAGTAGGTACGCACCTGACCTCGCTGGCACTGCCGGGCAAGGCAGAGAGTGTGGCGTCGCTCACC
  AGCCAGTGCAGCTACAGCAGCACCATCGTCCATGTGGGAGACAAGAAGCCGCAGCCGGAGTTAGAGATG
  GTGGAAGATGCTGCGAGTGGGCCAGAATCCCTGGACTGCCTGGCGGGCCCTGCCCTGGCCTGTGGTCTC
  AGCCAAGAGAAGGAGCCCTTCAAGAAGCTGGGCCTCACCAAGGAGGTACTCGCTGCACACACACAGAAG
  GAGGAGCAGAGCTTCCTGCAGAAGTTCAAAGAAATAAGAAAACTCAGCATTTTCCAGTCCCACTGCCAT
  TACTACTTGCAAGAAAGATCCAAGGGGCAGCCAAGTGAACGAACTGCCCCTGGACTAAGAAATACTTCC
  GGAATAGATTCACCTTGGAAAAAAACAGGAAAGAACAGAAAATTGAAGTCCAAGCGGGTCAAACCTCGA
  GACTCATCTGAGAGCACCGGATCTGGGGGGCCCGTGTCCGCCCGGCCCCCGCTGGTGGGCTTGAACGCC
  ACAGCCTGGTCACCCTCAGACACGTCCCAGTCCAGCTGCCCAGCCGTGCCCTTTCCCGCCCCAGTGCCA
  GCAGCTTATTCACTGCCCGTGTTTCCAGCGCCAGGGACTGTGGCAGCACCCCCGGCACCTCCCCACGCC
  AGCTTCACAGTGCCTGCTGTGCCCGTGGACCTCCAGCACCAGTTTGCAGTCCAGCCCCCACCTTTCCCT
  GCCCCTTTGGCGCCTGTCATGGCATTCATGCTACCCAGTTATTCCTTCCCCTCGGGGACCCCAAACCTG
  CCCCAGGCCTTCTTCCCCAGCCAGCCTCAGTTTCCGAGCCACCCCACACTCACATCCGAGATGGCCTCT
  GCCTCACAGCCTGAGTTCCCCAGCCGGACCTCGATCCCCAGACAGCCATGTGCTTGTCCAGCCACCCGG
  GCCACCCCACCATCGGCCATGGGTAGGGCCTCCCCACCGCTCTTTCAGTCCCGCAGCAGCTCGCCCCTG
  CAGCTCAACCTGCTGCAGCTGGAGGAAGCCCCTGAGGGTGGCACTGGAGCCATGGGGACCACAGGGGCC
  ACAGAGACAGCAGCTGTAGGGGCGGACTGCAAACCTGGCACTTCTCGGGACCAGCAGCCGAAGGCGCCT
  CTGACCCGTGATGAACCCTCAGACACACAGAACAGTGACGCCCTTTCCACGTCAAGCGGCCTCCTAAAC
  CTCCTGCTGAATGAGGACCTCTGCTCAGCCTCGGGCTCTGCTGCTTCGGAGTCTCTGGGCTCCGGCTCA
  CTGGGCTGCGACGCCTCCCCGAGTGGGGCAGGCAGTAGTGACACAAGTCATACCAGCAAATATTTTGGA
  AGCATTGACTCCTCAGAGAATAATCACAAAGCAAAAATGAACACTGGTATGGAAGAAAGTGAGCATTTC
  ATTAAGTGCGTCCTGCAGGATCCCATCTGGCTGCTGATGGCAGATGCGGACAGCAGCGTCATGATGACG
  TACCAGCTGCCTTCCCGAAATTTAGAAGCGGTTTTGAAGGAGGACAGAGAGAAGCTGAAGCTCCTACAG
  AAACTCCAGCCCAGGTTCACGGAGAGTCAGAAGCAGGAGCTGCGCGAGGTCCACCAGTGGATGCAGACG
  GGCGGCCTGCCCGCAGCCATCGACGTGGCAGAATGTGTTTACTGTGAAAACAAGGAAAAAGGTAATATT
  TGCATACCATATGAGGAAGATATTCCTTCTCTGGGACTCAGCGAAGTGTCGGACACCAAAGAAGACGAA
  AATGGATCCCCCTTGAATCACAGGATCGAAGAGCAGACGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022817
• Insert Size: 3768 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022817.2
• RefSeq Size: 6342 bp
• RefSeq ORF: 3768 bp
• Locus ID: 8864
• UniProt ID: O15055
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 136.6 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]