PER1 (NM_002616) Human Untagged Clone

CAT#: SC303227

PER1 (untagged)-Human period homolog 1 (Drosophila) (PER1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PER1
• Synonyms: hPER; PER; RIGUI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303227 representing NM_002616.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTGGCCCCCTAGAAGGGGCTGATGGGGGAGGGGACCCCAGGCCTGGGGAATCATTTTGTCCTGGG
  GGCGTCCCATCCCCTGGGCCCCCACAGCACCGGCCTTGCCCAGGCCCCAGCCTGGCCGATGACACCGAT
  GCCAACAGCAATGGTTCAAGTGGCAATGAGTCCAACGGGCATGAGTCTAGAGGCGCATCTCAGCGGAGC
  TCACACAGCTCCTCCTCAGGCAACGGCAAGGACTCAGCCCTGCTGGAGACCACTGAGAGCAGCAAGAGC
  ACAAACTCTCAGAGCCCATCCCCACCCAGCAGTTCCATTGCCTACAGCCTCCTGAGTGCCAGCTCAGAG
  CAGGACAACCCGTCCACCAGTGGCTGCAGCAGTGAACAGTCAGCCCGGGCAAGGACTCAGAAGGAACTC
  ATGACAGCACTTCGAGAGCTCAAGCTTCGACTGCCGCCAGAGCGCCGGGGCAAGGGCCGCTCTGGGACC
  CTGGCCACGCTGCAGTACGCACTGGCCTGTGTCAAGCAGGTGCAGGCCAACCAGGAATACTACCAGCAG
  TGGAGCCTGGAGGAGGGCGAGCCTTGCTCCATGGACATGTCCACCTATACCCTGGAGGAGCTGGAGCAC
  ATCACGTCTGAGTACACACTTCAGAACCAGGATACCTTCTCAGTGGCTGTCTCCTTCCTGACGGGCCGA
  ATCGTCTACATTTCGGAGCAGGCAGCCGTCCTGCTGCGTTGCAAGCGGGACGTGTTCCGGGGTACCCGC
  TTCTCTGAGCTCCTGGCTCCCCAGGATGTGGGAGTCTTCTATGGTTCCACTGCTCCATCTCGCCTGCCC
  ACCTGGGGCACAGGGGCCTCAGCAGGTTCAGGCCTCAGGGACTTTACCCAGGAGAAGTCCGTCTTCTGC
  CGTATCAGAGGAGGTCCTGACCGGGATCCAGGGCCTCGGTACCAGCCATTCCGCCTAACCCCGTATGTG
  ACCAAGATCCGGGTCTCAGATGGGGCCCCTGCACAGCCGTGCTGCCTGCTGATTGCAGAGCGCATCCAT
  TCGGGTTACGAAGCTCCCCGGATACCCCCTGACAAGAGGATTTTCACTACGCGGCACACACCCAGCTGC
  CTCTTCCAGGATGTGGATGAAAGGGCTGCCCCCCTGCTGGGCTACCTGCCCCAGGACCTCCTGGGGGCC
  CCAGTGCTCCTGTTCCTGCATCCTGAGGACCGACCCCTCATGCTGGCTATCCACAAGAAGATTCTGCAG
  TTGGCGGGCCAGCCCTTTGACCACTCCCCTATCCGCTTCTGTGCCCGCAACGGGGAGTATGTCACCATG
  GACACCAGCTGGGCTGGCTTTGTGCACCCCTGGAGCCGCAAGGTAGCCTTCGTGTTGGGCCGCCACAAA
  GTACGCACGGCCCCCCTGAATGAGGACGTGTTCACTCCCCCGGCCCCCAGCCCAGCTCCCTCCCTGGAC
  ACTGATATCCAGGAGCTGTCAGAGCAGATCCACCGGCTGCTGCTGCAGCCCGTCCACAGCCCCAGCCCC
  ACGGGACTCTGTGGAGTCGGCGCCGTGACATCCCCAGGCCCTCTCCACAGCCCTGGGTCCTCCAGTGAT
  AGCAACGGGGGTGATGCAGAGGGGCCTGGGCCTCCTGCGCCAGTGACTTTCCAGCAGATCTGTAAGGAT
  GTGCATCTGGTGAAGCACCAGGGCCAGCAGCTTTTTATTGAGTCTCGGGCCCGGCCTCAGTCCCGGCCC
  CGCCTCCCTGCTACAGGCACGTTCAAGGCCAAGGCCCTTCCCTGCCAATCCCCAGACCCAGAGCTGGAG
  GCGGGTTCTGCTCCCGTCCAGGCCCCACTAGCCTTGGTCCCTGAGGAGGCCGAGAGGAAAGAAGCCTCC
  AGCTGCTCCTACCAGCAGATCAACTGCCTGGACAGCATCCTCAGGTACCTGGAGAGCTGCAACCTCCCC
  AGCACCACTAAGCGTAAATGTGCCTCCTCCTCCTCCTATACCACCTCCTCAGCCTCTGACGACGACAGG
  CAGAGGACAGGTCCAGTCTCTGTGGGGACCAAGAAAGATCCGCCGTCAGCAGCGCTGTCTGGGGAGGGG
  GCCACCCCACGGAAGGAGCCAGTGGTGGGAGGCACCCTGAGCCCGCTCGCCCTGGCCAATAAGGCGGAG
  AGTGTGGTGTCCGTCACCAGTCAGTGTAGCTTCAGCTCCACCATCGTCCATGTGGGAGACAAGAAGCCC
  CCGGAGTCGGACATCATCATGATGGAGGACCTGCCTGGCCTAGCCCCAGGCCCAGCCCCCAGCCCAGCC
  CCCAGCCCCACAGTAGCCCCTGACCCAGCCCCAGACGCCTACCGTCCAGTGGGGCTGACCAAGGCCGTG
  CTGTCCCTGCACACACAGAAGGAAGAGCAAGCCTTCCTCAGCCGCTTCCGAGACCTGGGCAGGCTGCGT
  GGACTCGACAGCTCTTCCACAGCTCCCTCAGCCCTTGGCGAGCGAGGCTGCCACCACGGCCCCGCACCC
  CCAAGCCGCCGACACCACTGCCGATCCAAAGCCAAGCGCTCACGCCACCACCAGAACCCTCGGGCTGAA
  GCGCCCTGCTATGTCTCACACCCCTCACCCGTGCCACCCTCCACCCCCTGGCCCACCCCACCAGCCACT
  ACCCCCTTCCCAGCGGTTGTCCAGCCCTACCCTCTCCCAGTGTTCTCTCCTCGAGGAGGCCCCCAGCCT
  CTTCCCCCTGCTCCCACATCTGTGCCCCCAGCTGCTTTCCCCGCCCCTTTGGTGACCCCAATGGTGGCC
  TTGGTGCTCCCTAACTATCTGTTCCCAACCCCATCCAGCTATCCTTATGGGGCACTCCAGACCCCTGCT
  GAAGGGCCTCCCACTCCTGCCTCGCACTCCCCTTCTCCATCCTTGCCCGCCCTCGCCCCGAGTCCTCCT
  CACCGCCCGGACTCTCCACTGTTCAACTCGAGATGCAGCTCTCCACTCCAGCTCAATCTGCTGCAGCTG
  GAGGAGCTCCCCCGTGCTGAGGGGGCTGCTGTTGCAGGAGGCCCTGGGAGCAGTGCCGGGCCCCCACCT
  CCCAGTGCGGAGGCTGCTGAGCCAGAGGCCAGACTGGCGGAGGTCACTGAGTCCTCCAATCAGGACGCA
  CTTTCCGGCTCCAGTGACCTGCTCGAACTTCTGCTGCAAGAGGACTCGCGCTCCGGCACAGGCTCCGCA
  GCCTCGGGCTCCTTGGGCTCTGGCTTGGGCTCTGGGTCTGGTTCAGGCTCCCATGAAGGGGGCAGCACC
  TCAGCCAGCATCACTCGCAGCAGCCAGAGCAGCCACACAAGCAAATACTTTGGCAGCATCGACTCTTCC
  GAGGCTGAGGCTGGGGCTGCTCGGGGCGGGGCTGAGCCTGGGGACCAGGTGATTAAGTACGTGCTCCAG
  GATCCCATTTGGCTGCTCATGGCCAATGCTGACCAGCGCGTCATGATGACCTACCAGGTGCCCTCCAGG
  GACATGACCTCTGTGCTGAAGCAGGATCGGGAGCGGCTCCGAGCCATGCAGAAGCAGCAGCCTCGGTTT
  TCTGAGGACCAGCGGCGGGAACTGGGTGCTGTGCACTCCTGGGTCCGGAAGGGCCAACTGCCTCGGGCT
  CTTGATGTGATGGCCTGTGTGGACTGTGGGAGCAGCACCCAAGATCCTGGTCACCCTGATGACCCACTC
  TTCTCAGAGCTGGATGGACTGGGGCTGGAGCCCATGGAAGAGGGTGGAGGCGAGCAGGGCAGCAGCGGT
  GGCGGCAGTGGTGAGGGAGAGGGCTGCGAGGAGGCCCAAGGCGGGGCCAAGGCTTCAAGCTCTCAGGAC
  TTGGCTATGGAGGAGGAGGAAGAAGGCAGGAGCTCATCCAGTCCAGCCTTACCTACAGCAGGAAACTGC
  ACCAGCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002616
• Insert Size: 3873 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002616.2
• RefSeq Size: 4717 bp
• RefSeq ORF: 3873 bp
• Locus ID: 5187
• UniProt ID: O15534
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 136.2 kDa
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]