MLYCD (NM_012213) Human Untagged Clone

CAT#: SC128266

MLYCD (untagged)-Human malonyl-CoA decarboxylase (MLYCD), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MLYCD
• Synonyms: MCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012213 edited
  ATGCGAGGCTTCGGGCCAGGCTTGACGGCCAGGCGTCTCCTCCCGCTGCGGTTGCCCCCG
  CGGCCGCCCGGGCCCCGGCTGGCGAGCGGGCAGGCGGCCGGCGCCCTGGAGCGGGCCATG
  GACGAGCTGCTGCGCCGCGCGGTGCCGCCGACGCCGGCCTACGAGCTGCGCGAGAAGACA
  CCGGCGCCCGCCGAGGGTCAGTGCGCGGACTTCGTGAGCTTCTACGGTGGGCTGGCCGAG
  ACGGCCCAGCGGGCCGAACTGCTGGGCCGCCTGGCGCGGGGCTTCGGCGTGGACCACGGC
  CAGGTGGCGGAGCAGAGCGCCGGCGTGCTCCATCTGCGCCAGCAGCAGCGGGAGGCGGCG
  GTGCTGCTGCAGGCCGAGGACCGGCTGCGCTACGCGCTGGTGCCGCGCTATCGCGGCCTC
  TTCCACCACATCAGCAAGCTGGGCGGCGGCGTGCGCTTCCTGGTGCAGCTGCGGGCCGAC
  CTGCTGGAGGCGCAGGCCCTCAAGCTGGTGGAGGGGCCGGACGTCCGGGAAATGAATGGG
  GTGCTGAAAGGAATGCTCTCAGAATGGTTTTCCTCCGGGTTCCTGAACCTAGAACGGGTT
  ACCTGGCATTCACCGTGTGAAGTGCTTCAGAAAATCAGTGAGGCTGAGGCTGTGCATCCT
  GTAAAAAACTGGATGGACATGAAGCGCCGCGTTGGGCCCTACAGAAGGTGTTACTTCTTT
  TCTCACTGTTCGACCCCTGGGGAGCCCCTGGTCGTTTTGCACGTGGCACTGACTGGTGAC
  ATCTCCAGCAACATCCAGGCAATCGTGAAGGAACATCCTCCATCAGAAACAGAAGAGAAG
  AACAAAATCACTGCTGCGATCTTTTATTCCATCAGCTTGACCCAGCAGGGACTCCAAGGG
  GTGGAGCTGGGAACATTCCTCATAAAGCGAGTCGTCAAGGAGTTGCAGAGAGAGTTTCCT
  CACCTTGGGGTGTTTTCAAGTCTGTCACCTATACCTGGTTTCACCAAATGGCTTCTGGGG
  CTTCTGAACTCGCAAACGAAGGAGCATGGGAGGAATGAACTCTTTACAGATTCGGAATGT
  AAGGAAATCTCGGAGATCACAGGTGGCCCCATTAACGAGACCCTCAAGCTCCTCCTCAGC
  AGCAGCGAGTGGGTGCAGTCGGAGAAGCTGGTGCGGGCGCTGCAGACTCCGCTGATGAGG
  CTGTGCGCCTGGTACCTGTATGGAGAGAAGCACCGCGGCTACGCGCTGAACCCCGTGGCC
  AACTTCCACCTGCAGAACGGGGCGGTGCTGTGGCGCATCAACTGGATGGCGGATGTGAGC
  CTCAGAGGCATCACCGGCTCCTGCGGCCTGATGGCCAACTACCGCTACTTCCTGGAGGAG
  ACGGGCCCCAACAGCACCTCCTACCTCGGCTCCAAGATCATCAAAGCCTCTGAGCAGGTC
  CTCAGCCTAGTGGCCCAGTTTCAAAAGAACAGCAAGCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012213 unedited
  NTTTTTGGGNGGCGTTANCATTTGTTATACCACTTACTATAGGCGGCCGCGACTTCATGC
  GAGGCTTCGGGCCAGGCTTGACGGCCAGGCGTCTCCTCCCGCTGCGGTTGCCCCCGCGGC
  CGCCCGGGCCCCGGCTGGCGAGCGGGCAGGCGGCCGGCGCCCTGGAGCGGGCCATGGACG
  AGCTGCTGCGCCGCGCGGTGCCGCCGACGCCGGCCTACGAGCTGCGCGAGAAGACACCGG
  CGCCCGCCGAGGGTCAGTGCGCGGACTTCGTGAGCTTCTACGGTGGGCTGGCCGAGACGG
  CCCAGCGGGCCGAACTGCTGGGCCGCCTGGCGCGGGGCTTCGGCGTGGACCACGGCCAGG
  TGGCGGAGCAGAGCGCCGGCGTGCTCCATCTGCGCCAGCAGCAGCGGGAGGCGGCGGTGC
  TGCTGCANGCCGAGGACCGGCTGCGCTACGCGCTGGTGCCGCGCTATCGCGGCCTCTTCC
  ACCACATCANCAAGCTGGGCGGCGGCGTGCGCTTCCTGGTGCAGCTGCGGGCCGACCTGC
  TGGAGGCGCAGGCCCTCAAGCTGGTGGAGGGCCCCGGACGTCCGGAAAATGATGGNGTGC
  TGAAAGGAATGCTCTCAGAAGGGTTTCCCTCCGGTTTCCTGAACCTAGNACGGGTTACCT
  GGCATTCACCGTGGTGAGTGCCTTCAAAAATCAGTGAGGCTGAGGCTGGGCATCCCTGTA
  AAAAACTGGTTGGACATGAAACCCCCCGTTGGGCCCTACAAAAGTGTACCTTCTTTCTCA
  CTGTTCGACCCTGGGGAGCCCCTGGTCCTTTTGACTGTGCCTGAATTGGGGAATTTCAGA
  AAATTCAGCATATGGGAAGAACCTCCTCCTTCGAAACGGAAAAAAAAAAAAATCCCGG
  
  3' Read Nucleotide Sequence

  >Reverse primer walk for NM_012213 unedited
  CCCTGGGTGCTGTCACGCCCAGTGTGAGGAAATTGAGGCCTGGCCTCACGTGTGGACCGG
  CTGCGGGACAAGAACACAGTCAACTTTGGCTTTGCTTGGAAAGCTGCTTCAGATACATAA
  CTCCCGGCCCCTCCTGAAAATGATCGTTTTCTTAGCCGGGGCCCTGTGCTTTAGGAGAGG
  TTTACTGTCAGAGCTTGCTGTTCTTTTGAAACTGGGCCACTAGGCTGAGGACCTGCTCAG
  AGGCTTTGATGATCTTGGAGCCGAGGTAGGAGGTGCTGTTGGGGCCCGTCTCCTCCAGGA
  AGTAGCGGTAGTTGGCCATCAGGCCGCAGGAGCCGGTGATGCCTCTGAGGCTCACATCCG
  CCATCCAGTTGATGCGCCACAGCACCGCCCCGTTCTGCAGGTGGAAGTTGGCCACGGGGT
  TCAGCGCGTAGCCGCGGTGCTTCTCTCCATACAGGTACCAGGCGCACAGCCTCATCAGCG
  GAGTCTGCAGCGCCCGCACCAGCTTCTCCGACTGCACCCACTCGCTGCTGCTGAGGAGGA
  GCTTGAGGGTCTCGTTAATGGGGCCACCTGTGATCTCCGAGATTTCCTTACATTCCGAAT
  CTGTAAAGAGTTCATTCCTCCCATGCTCCTTCGTTTGCGAGTTCAGAAGCCCCAGAAGCC
  ATTTGGTGAAACCAGGTATAGGTGACAGACTTGAAAACACCCCAAGGTGAGGAAACTCTC
  TCTGNCACTCCTTGACGACTCGCTTTATGAGGAATGTTCCCAGCTCCACCCCTTGGAGTC
  CCTGCTGGGTCAAGCTGATGGAATAAAAGATCGCAGCAGNGATTTTGTTCCTCCCCTCCT
  GTTCTGAGGGAAGATGTTCCTTCACGATGCCTGGATGTTGCTGGAGATGTCACCATCAGT
  GCCCCGTGCAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_012213
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012213.2, NP_036345.2
• RefSeq Size: 2211 bp
• RefSeq ORF: 1482 bp
• Locus ID: 23417
• UniProt ID: O95822
• Cytogenetics: 16q23.3
• Domains: MCD
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism
• Gene Summary: The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]