ABAT (NM_020686) Human Untagged Clone

CAT#: SC127432

ABAT (untagged)-Human 4-aminobutyrate aminotransferase (ABAT), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABAT
• Synonyms: GABA-AT; GABAT; NPD009
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020686, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCCATGTTGCTCGCCCAGCGCCTGGCCTGCAGCTTCCAGCACAGCTACCGCCTGCTGGTGCCTG
  GATCCAGACACATTAGTCAAGCTGCAGCCAAAGTCGACGTTGAATTTGATTATGATGGGCCTCTGATGAA
  GACGGAAGTCCCAGGGCCTAGATCTCAGGAGTTAATGAAACAGCTGAATATAATTCAGAATGCAGAGGCT
  GTGCATTTTTTCTGCAATTACGAAGAGAGCCGAGGCAATTACCTGGTTGATGTGGACGGCAACCGAATGC
  TGGATCTTTATTCCCAGATCTCCTCTGTCCCCATAGGTTACAGCCACCCCGCCCTGCTGAAACTCATCCA
  ACAGCCTCAAAATGCGAGCATGTTTGTCAACAGACCCGCCCTCGGAATCCTGCCTCCGGAGAACTTTGTG
  GAGAAGCTCCGGCAGTCCTTGCTCTCGGTGGCTCCCAAAGGGATGTCCCAGCTCATCACCATGGCCTGCG
  GCTCCTGCTCCAATGAAAACGCCTTAAAGACCATCTTCATGTGGTACCGGAGCAAGGAAAGAGGGCAGAG
  GGGCTTCTCCCAGGAGGAGCTGGAGACGTGCATGATTAACCAGGCCCCTGGCTGCCCCGACTACAGCATC
  CTCTCCTTCATGGGCGCGTTCCATGGGAGGACCATGGGTTGCTTAGCGACCACGCACTCTAAAGCCATTC
  ACAAGATCGACATCCCTTCCTTTGACTGGCCCATCGCACCGTTCCCACGGCTGAAATACCCTCTGGAAGA
  GTTTGTGAAAGAGAACCAACAGGAGGAGGCCCGCTGTCTGGAAGAGGTGGAGGATCTGATTGTGAAATAT
  CGGAAAAAGAAGAAGACGGTGGCCGGGATCATCGTGGAGCCCATCCAGTCCGAGGGTGGAGACAACCACG
  CATCCGATGACTTCTTTCGGAAGCTGAGAGACATCGCCAGGAAGCATGGCTGCGCCTTCTTGGTGGACGA
  GGTCCAGACCGGAGGAGGCTGCACGGGCAAGTTCTGGGCCCATGAGCACTGGGGCCTGGATGACCCAGCA
  GACGTGATGACCTTCAGCAAGAAGATGATGACTGGGGGCTTCTTCCACAAGGAGGAGTTCAGGCCTAATG
  CTCCCTACCGGATCTTCAACACCTGGCTGGGGGACCCGTCCAAGAACCTGTTGCTGGCTGAGGTCATCAA
  CATCATCAAGCGGGAGGACCTGCTAAATAATGCAGCCCATGCCGGGAAGGCCCTGCTCACAGGACTGCTG
  GACCTCCAGGCCCGGTACCCCCAGTTCATCAGCAGGGTGAGAGGACGAGGCACCTTTTGCTCCTTCGATA
  CTCCCGATGATTCCATACGGAATAAGCTCATTTTAATTGCCAGAAACAAAGGTGTGGTGTTGGGTGGCTG
  TGGTGACAAATCCATTCGTTTCCGTCCCACGCTGGTCTTCAGGGATCACCACGCTCACCTGTTCCTCAAT
  ATTTTCAGTGACATCTTAGCAGACTTCAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020686 unedited
  CACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCTCCCTTGCGCACTTGCCGGGTACCA
  GCTGAGTGCATCCTTTTCCCAAACCTGCTCTGCTTCCAAGGGGCCCTTTTCTGTCTCCTC
  CACCTGTCCCCATGTTTTGGGGACTGCCTGGAAATCCGGGACACCCTCCTGAGGAGGGAG
  CTGGTGCCCAACGGCACCTCTTAGGATGAACCCAGCCAGCTGCGTTCCTGGCCTCTGCAG
  AATGGCATACAGAAGGCATTTTAAGCAAGCGAGCTGAGCTCGCCGCAGGACTCCGACTAC
  CAGGCACCGGTGGCAGCACGCAAAGGGTGTCCCTGTCCCTCAAGGGGTCATGGCCTCCAT
  GTTGCTCGCCCAGCGCCTGGCCTGCAGCTTCCAGCACAGCTACCGCCTGCTGGTGCCTGG
  ATCCAGACACATTAGTCAAGCTGCAGCCAAAGTTCGACGTTGAATTTGATTATGATGGGC
  CTCTGATGAAGACGGAAGTCCCAGGGCCTAGATCTCAGGAGTTAATGAAACAGCTGAATA
  TAATTCAGAATGCAGAGGCTGTGCATTTTTTCTGCAATTACGAAGAGAGCCGAGGCAATT
  ACCTGGTTTGATGGTGGACGGCAACCGAATGGCTGGATCTTTATTCCCAGATCTCCTCTG
  NTCCCATAGGNTACAGCCACCCCGCCCTGCTGAAACTCATNCAACAGCCTCAAATGCGAG
  CCTTGTTTGTCAACAGACCCGCCTCGGAATCCTGCTNCGGANAACNTTGGGGANAACTTN
  CNGCAGTCTTGNTTTTCGGGGCTCAAAANNGGGATGTCCCANTTATAACCATGGGCTGGG
  GGTTCTGCTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020686 unedited
  CAGCTTGCCCGCGGCCGCAATCTAGGATCGGTTTTTTTTTTTTTTTTTTTAAATTATAAA
  TTTTATTTAAGAATACTGACTAACACAGGAAAACAGATTTAATTCATGGAATTGTGCATA
  TGGTCATCCGTTACATTGTGACATGTTAATTTTTTTTTTATCATTTATTGGCACTGTCAA
  CAGATTACTTGTGAACAAGATCACTTTGTACGCTTAAGTCTGCGATGCTACTTAGCTATG
  GTTTTCTACCATGAGCTTATATATAGATAGGTGTAGGTATGTAGATACATTAATGCTATA
  CACAATTTTGCATGGTTACTGAGCGTCAGTAAAAATTATGAAAAAACACCCATTTATAAT
  AAAAGTGAGGATGTACTAAGACTTGCTATTACTGGACCTTGTTTTCTGTAAAAGTGATGA
  CACTTGCTGGACGGTTACTAAACTCTATGGCACTAATGTATGATGGATTCATTTCCAGAC
  TGTCGGCCACGGAAGCACTTCTTCATGGCCTCTGCCCTGGACAGCAGCCTGTCCTCCGGG
  CTCCCCATGTTTTTACCAGCTTCTGCTGAGTTTCTACAATCTTGAGCTCTGCTGAGAATT
  CTTTTCCTTGAAATTCTTCTACCTAAAGCCCCAGCCCCCAAAAGAGCATGTCTCAGGAAC
  TCATTATGCCCTGAGTCAACANGAACTTGTTGATAAATGGCTTAAAAGTTTTTACAAGAA
  AGTACTTCCCTTGGTAAGGAGTAAATAATAGCTCTGGAATTTTCCAGATAAAACTATTTN
  ATTTNCTCTGTCAGTGGCCCCATGGGGGAGAGAACGAAATTATTGGAGCCTCTCTCCCTA
  CCAAAGAGAGCCACTTNTTCTGGNTGTGCCCCTGNCCTTAAAACCCTTTTGAACTCCGCA
  GACCCATACTGAATTATTTGCACCCAATGCCTAAGTTTTTCAGGAGAAGACCTTACTTAA
  GCCCAATAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020686
• Insert Size: 5270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020686.3, NP_065737.2
• RefSeq Size: 4830 bp
• RefSeq ORF: 1503 bp
• Locus ID: 18
• UniProt ID: P80404
• Cytogenetics: 16p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) has a more distal alternate 5' UTR. Variants 1-3 encode the same protein.