CYP2A7 (NM_000764) Human Untagged Clone

CAT#: SC126922

CYP2A7 (untagged)-Human cytochrome P450, family 2, subfamily A, polypeptide 7 (CYP2A7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP2A7
• Synonyms: CPA7; CPAD; CYP2A; CYPIIA7; P450-IIA4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000764, the custom clone sequence may differ by one or more nucleotides
  
  
  GGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACT
  CACTATAGGGCGGCCGCGAATTCGGCACCAGGCTGGCCTCAGGGATGCTTCTGGTGGCCTTGCTGGTCTG
  CCTGACTGTGATGGTCTTGATGTCTGTTTGGCAGCAGAGGAAGAGCAAGGGGAAGCTGCCTCCGGGACCC
  ACCCCATTGCCCTTCATTGGAAACTACCTGCAGCTGAACACAGAGCAGATGTACAACTCCCTCATGAAGA
  TCAGTGAGCGCTATGGCCCCGTGTTCACCATTCACTTGGGGCCCCGGCGGGTCGTGGTGCTGTGTGGACA
  TGATGCCGTCAGGGAGGCTCTGGTGGACCAGGCTGAGGAGTTCAGCGGGCGAGGCGAGCAAGCCACCTTC
  GACTGGGTCTTCAAAGGCTATGGCGTGGTATTCAGCAACGGGGAGCGCGCCAAGCAGCTCCGGCGCTTCT
  CCATCGCCACCCTGCGGGACTTCGGGGTGGGCAAGCGAGGCATCGAGGAGCGCATCCAGGAGGAGGCGGG
  CTTCCTCATCGACGCCCTCCGGGGCACTGGCGGCGCCAATATCGATCCCACCTTCTTCCTGAGCCGCACA
  GTCTCCAATGTCATCAGCTCCATTGTCTTTGGGGACCGCTTTGACTATAAGGACAAAGAGTTCCTGTCAC
  TGTTGCGCATGATGCTAGGAATCTTCCAGTTCACGTCAACCTCCACGGGGCAGCTCTATGAGATGTTCTC
  TTCGGTGATGAAACACCTGCCAGGACCACAGCAACAGGCCTTTCAGTTGCTGCAAGGGCTGGAGGACTTC
  ATAGCCAAGAAGGTGGAGCACAACCAGCGCACGCTGGATCCCAATTCCCCACGGGACTTCATTGACTCCT
  TTCTCATCCGCATGCAGGAGGAGGAGAAGAACCCCAACACGGAGTTCTACTTGAAAAACCTGGTGATGAC
  CACGTTGAACCTCTTCATTGGGGGCACCGAGACCGTCAGCACCACCCTGCGCTATGGCTTCTTGCTGCTC
  ATGAAGCACCCAGAGGTGGAGGCCAAGGTCCATGAGGAGATTGACAGAGTGATCGGCAAGAACCGGCAGC
  CCAAGTTTGAGGACCGGGCCAAGATGCCCTACATGGAGGCAGTGATCCACGAGATCCAAAGATTTGGAGA
  CGTGATCCCCATGAGTTTGGCCCGCAGAGTCAAAAAGGACACCAAGTTTCGGGATTTCTTCCTCCCTAAG
  GGCACCGAAGTGTACCCTATGCTGGGCTCTGTGCTGAGAGACCCCAGTTTCTTCTCCAACCCCCAGGACT
  TCAATCCCCAGCACTTCCTGAATGAGAAGGGGCAGTTTAAGAAGAGTGATGCTTTTGTGCCCTTTTCCAT
  CGGAAAGCGGAACTGTTTCGGAGAAGGCCTGGCCAGAATGGAGCTCTTTCTCTTCTTCACCACCGTCATG
  CAGAACTTCCGCCTCAAGTCCTCCCAGTCACCTAAGGACATTGACGTGTCCCCCAAACACGTGGGCTTTG
  CCACGATCCCACGAAACTACACCATGAGCTTCCTGCCCCGCTGAGCGAGGGCTGTGCCGGTGCAGGTCTG
  GTGGGCGGGGCCAGGGAAAGGCGGGGTCAGGGCGGGGTTCGCGGAAGAGGCGGGTATAAGAATGGGGGGA
  AGAT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000764 unedited
  NGTTCAAAATATTTGTATACGAACTCACTATAGGGCGGCCGCGACATTCGCACCAGCTGG
  CCTCAGGATGCTTCTGGTGGCCTTGCTGGTCTGCCTGACTGTGATGGTCTTGATGTCTGT
  TTGGCAGCAGAAGAAGAGCAAGGGGAAGCTGCCTCCGGGACCCACCCCATTGCCCTTTAT
  TGGAAACTACCTGCAGCTGAAACACAGAGCAGATGTACAACTCCCTCATGAAGACTCAGT
  GAGCGCTATGGCCCCGTGTTCACCATTCAACTTGGGGCCCCGGCGGGTCGTGGTGCTGTG
  TGGACAATGATGCCGTCAGGGAGGCTCTGGTGGACCAAGGCTGAGGAGTTCAAGCGGGCG
  AAGGCGAGCAAGCCACCTTCGACTGGGTCTTCAAAGGCTATGGCGTGGTATTCAAGCAAC
  GGGGAGCGCGCCAAGCAGCTCCGGCGCTTCTCCATTCGCCACCCTGCGGGAATTCGGGGT
  GGGCAAGCGAAGGCATCGAGGAGCGCATCCAGGAAGAGGCGGGCTTCCTCATCGACGCCC
  TTCCGGGGCACTGGCGGCGCCAATATCGATCCCACCTTTTTCCTTGAGCCGCACAAGTCT
  CCAATGTCATCAGCTCCATTGTCTTTGGGGAACGCTTTGACTATAAGGAACAAAAGTTCC
  TGTCACTGTTGCGCATGATGCTAGGAATCTTCCAGTTCACGTCAACCTCCACGGNGCAGC
  TCTATGAAGAAGTTCTCTTTCGTGATAAAACACCTGCCAAGAACACAGCAACAGNCCTTT
  CAGTGCTGCAAGGNNCTGGAGAACTCATAGCCAGAAAGGTGAAGCACAACAGCGACGGCT
  TGATNCCAATTNCCACNGAACTTCATTGACTCTTTCTATCGCTGNCAGGAGAAGAAAAAA
  ACCAAAACGGATTCTACTTGAAAACTGTATGAACACGTGAACTTTNATTTGGGGCACCAA
  AACGTACACAACTGGCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000764 unedited
  NNTTTTTTTNNNNNNNTTTTTGACTNTGNACCGCGGNCCGCATACNANGATCGAGTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTCAGGAAATAAAACTGCTATTATTACTACTCTTCA
  TAGCATAATGTAAGGGTTTCCTTCCTCTCATCCCAGCTCGGAAGCACCTTATCAAGGTGA
  ACTGAGCCCCTTCTGTTTCTTCTCTTCCCTCTAGCCACCACGCCCCTTCCTTTCCCGCAT
  CTTCCCCCCATTCTTATACCCGCCTTTTCCGCGAACCCCGCCCTGACCCCGCCTTTCCCT
  GGCCCCGCCCACCAAACCTGCACCGGCACAGCCCTCGCTCAGCGGGGCAGGAAGCTCATG
  GTGTAGTTTCGGGGGATCGGGGCAAAACCCACGTGTTTGGGGGACACCTCAATGGCCTTA
  AGGGACTGGGAGGACTTGAGGCGGAAGTTCTTCATGACGGGGGTGAAAAAGAAAAAGAAC
  TCCATTCTGGCCAGGCCTTCTTCGAACAGTTCCGCTTTCCGATGGAAAAGGGGCCAAAAG
  CATTACTCTTTTTAAACTGGCCCTTCTCATTCAAGAAGTGCTGGGGATTGAAGTCCTGGG
  GGTTGGAAAAAAAACTGGGGTCTTTTACCACAGAGCCCCACCTAAGGTCACTTTCCGGGC
  CCTTAGGGAGGAAAAAATCCCAAACTTGGGGGCCTTTTTGACTTTGGGGCCAAACTCTGG
  GGAACACGTCTCCCAAATTTTGGATCCGGGGGACACTGCCCCCTGTAGGCATTTTGGCCC
  GTCCCTAAACTGGGCTGCCGGTTTTGGCGAAACTCTGTGAATTCCCTATGGACCTTGGCC
  TCACCTTTGTGCCTCATGACCACAAGAACCTACGCACAGTGTGCCCACGGTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000764
• Insert Size: 1870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000764.2, NP_000755.2
• RefSeq Size: 2281 bp
• RefSeq ORF: 1485 bp
• Locus ID: 1549
• UniProt ID: P20853
• Cytogenetics: 19q13.2
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Caffeine metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Retinol metabolism
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. This gene, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).