CYP21A2 (NM_000500) Human Untagged Clone

CAT#: SC125487

CYP21A2 (untagged)-Human cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP21A2
• Synonyms: CA21H; CAH1; CPS1; CYP21; CYP21B; P450c21B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000500 edited
  GTCAAGGGAGGCCCCAAAACAGTCTACACAGCAGGAGGGATGGCTGGGGCTCTTGAGCTA
  TAAGTGGCACCTCAGGGCCCTGACGGGCGTCTCGCCATGCTGCTCCTGGGCCTGCTGCTG
  CTGCTGCCCCTGCTGGCTGGCGCCCGCCTGCTGTGGAACTGGTGGAAGCTCCAGAGCCTC
  CACCTCCCGCCTCTTGCCCCGGGCTTCTTGCACTTGCTGCAGCCCGACCTCCCAATCTAT
  CTGCTTGGCCTGACTCAGAAATTCGGGCCCATCTACAGGCTCCACCTTGGGCTGCAAGAT
  GTGGTGGTGCTGAACTCCAAGAGGACCATTGAGGAAGCCATGGTCAAAAAGTGGGCAGAC
  TTTGCTGGCAGACCTGAGCCACTTACCTACAAGCTGGTGTCTAGGAACTACCCGGACCTG
  TCCTTGGGAGACTACTCCCTGCTCTGGAAAGCCCACAAGAAGCTCACCCGCTCAGCCCTG
  CTGCTGGGCATCCGTGACTCCATGGAGCCAGTGGTGGAGCAGCTGACCCAGGAGTTCTGT
  GAGCGCATGAGAGCCCAGCCCGGCACCCCTGTGGCCATTGAGGAGGAATTCTCTCTCCTC
  ACCTGCAGCATCATCTGTTACCTCACCTTCGGAGACAAGATCAAGGACGACAACTTAATG
  CCTGCCTATTACAAATGTATCCAGGAGGTGTTAAAAACCTGGAGCCACTGGTCCATCCAA
  ATTGTGGACGTGATTCCCTTTCTCAGGTTCTTCCCCAATCCAGGTCTCCGGAGGCTGAAG
  CAGGCCATAGAGAAGAGGGATCACATCGTGGAGATGCAGCTGAGGCAGCACAAGGAGAGC
  CTCGTGGCAGGCCAGTGGAGGGACATGATGGACTACATGCTCCAAGGGGTGGCGCAGCCG
  AGCATGGAAGAGGGCTCTGGACAGCTCCTGGAAGGGCACGTGCACATGGCTGCAGTGGAC
  CTCCTGATCGGTGGCACTGAGACCACAGCAAACACCCTCTCCTGGGCCGTGGTTTTTTTG
  CTTCACCACCCTGAGATTCAGCAGCGACTGCAGGAGGAGCTAGACCACGAACTGGGCCCT
  GGTGCCTCCAGCTCCCGGGTCCCCTACAAGGACCGTGCACGGCTGCCCTTGCTCAATGCC
  ACCATCGCCGAGGTGCTGCGCCTGCGGCCCGTTGTGCCCTTAGCCTTGCCCCACCGCACC
  ACACGGCCCAGCAGCATCTCCGGCTACGACATCCCTGAGGGCACAGTCATCATTCCGAAC
  CTCCAAGGCGCCCACCTGGATGAGACGGTCTGGGAGAGGCCACATGAGTTCTGGCCTGAT
  CGCTTCCTGGAGCCAGGCAAGAACTCCAGAGCTCTGGCCTTCGGCTGCGGTGCCCGCGTG
  TGCCTGGGCGAGCCGCTGGCGCGCCTGGAGCTCTTCGTGGTGCTGACCCGACTGCTGCAG
  GCCTTCACGCTGCTGCCCTCCGGGGACGCCCTGCCCTCCCTGCAGCCCCTGCCCCACTGC
  AGTGTCATCCTCAAGATGCAGCCTTTCCAAGTGCGGCTGCAGCCCCGGGGGATGGGGGCC
  CACAGCCCGGGCCAGAGCCAGTGATGGGGCAGGACCGATGCCAGCCGGGTACCTCAGTTT
  CTCCTTTATTGCTCCTGTACGAACCCCTCCCCTCCCCCCTGTAAACACAGTGCTGCGAGA
  TCGCTGGCAGAGAAGGCTTCCTCCAGCGGCTGGGTGGTGAAGGACCCTGGCTCTTCTCTC
  GGGGCGACCCCTCAGTGCTCGGCAGTCATACTGGGGTGCGAGAGAGGTGGGCAGCAGCTC
  AGCCTCCCCCCGCTGGGGAGCGAAAGTTTCTTGGTCTCAGCTTCATTTCCGTGAAGGGCA
  CCGAGAACTCGAAGCCCTTCCAGTGGTACCAGCTCACTCCCTGGGAAAGGGGTTGTCAAG
  AGAGAGTCAAAGCCGGATGTCCCATCTGCTCTTCCCGTTCCCCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_000500
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000500.5, NP_000491.2
• RefSeq Size: 2114 bp
• RefSeq ORF: 1488 bp
• Locus ID: 1589
• UniProt ID: P08686
• Cytogenetics: 6p21.33
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (a).