ABCB5 (NM_178559) Human Untagged Clone

CAT#: SC125267

ABCB5 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 (ABCB5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCB5
• Synonyms: ABCB5alpha; ABCB5beta; EST422562
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_178559 edited
  ATGGTGGATGAGAATGACATCAGAGCTTTAAATGTGCGGCATTATCGAGACCATATTGGA
  GTGGTTAGTCAAGAGCCTGTTTTGTTCGGGACCACCATCAGTAACAATATCAAGTATGGA
  CGAGATGATGTGACTGATGAAGAGATGGAGAGAGCAGCAAGGGAAGCAAATGCGTATGAT
  TTTATCATGGAGTTTCCTAATAAATTTAATACATTGGTAGGGGAAAAAGGAGCTCAAATG
  AGTGGAGGGCAGAAACAGAGGATCGCAATTGCTCGTGCCTTAGTTCGAAACCCCAAGATT
  CTGATTTTAGATGAGGCTACGTCTGCCCTGGATTCAGAAAGCAAGTCAGCTGTTCAAGCT
  GCACTGGAGAAGGCGAGCAAAGGTCGGACTACAATCGTGGTAGCACACCGACTTTCTACT
  ATTCGAAGTGCAGATTTGATTGTGACCCTAAAGGATGGAATGCTGGCGGAGAAAGGAGCA
  CATGCTGAACTAATGGCAAAACGAGGTCTATATTATTCACTTGTGATGTCACAGGATATT
  AAAAAAGCTGATGAACAGATGGAGTCAATGACATATTCTACTGAAAGAAAGACCAACTCA
  CTTCCTCTGCACTCTGTGAAGAGCATCAAGTCAGACTTCATTGACAAGGCTGAGGAATCC
  ACCCAATCTAAAGAGATAAGTCTTCCTGAAGTCTCTCTATTAAAAATTTTAAAGTTAAAC
  AAGCCTGAATGGCCTTTTGTGGTTCTGGGGACATTGGCTTCTGTTCTAAATGGAACTGTT
  CATCCAGTATTTTCCATCATCTTTGCAAAAATTATAACCATGTTTGGAAATAATGATAAA
  ACCACATTAAAGCATGATGCAGAAATTTATTCCATGATATTCGTCATTTTGGGTGTTATT
  TGCTTTGTCAGTTATTTCATGCAGGGATTATTTTACGGCAGAGCAGGGGAAATTTTAACG
  ATGAGATTAAGACACTTGGCCTTCAAAGCCATGTTATATCAGGATATTGCCTGGTTTGAT
  GAAAAGGAAAACAGCACAGGAGGCTTGACAACAATATTAGCCATAGATATAGCACAAATT
  CAAGGAGCAACAGGTTCCAGGATTGGCGTCTTAACACAAAATGCAACTAACATGGGACTT
  TCAGTTATCATTTCCTTTATATATGGATGGGAGATGACATTCCTGATTCTGAGTATTGCT
  CCAGTACTTGCCGTGACAGGAATGATTGAAACCGCAGCAATGACTGGATTTGCCAACAAA
  GATAAGCAAGAACTTAAGCATGCTGGAAAGATAGCAACTGAAGCTTTGGAGAATATACGT
  ACTATAGTGTCATTAACAAGGGAAAAAGCCTTCGAGCAAATGTATGAAGAGATGCTTCAG
  ACTCAACACAGAAATACCTCGAAGAAAGCACAGATTATTGGAAGCTGTTATGCATTCAGC
  CATGCCTTTATATATTTTGCCTATGCGGCAGGGTTTCGATTTGGAGCCTATTTAATTCAA
  GCTGGACGAATGACCCCAGAGGGCATGTTCATAGTTTTTACTGCAATTGCATATGGAGCT
  ATGGCCATCGGAGAAACGCTCGTTTTGGCTCCTGAATATTCCAAAGCCAAATCGGGGGCT
  GCGCATCTGTTTGCCTTGTTGGAAAAGAAACCAAATATAGACAGCCGCAGTCAAGAAGGG
  AAAAAGCCAGACACATGTGAAGGGAATTTAGAGTTTCGAGAAGTCTCTTTCTTCTATCCA
  TGTCGCCCAGATGTTTTCATCCTCCGTGGCTTATCCCTCAGTATTGAGCGAGGAAAGACA
  GTAGCATTTGTGGGGAGCAGCGGCTGTGGGAAAAGCACTTCTGTTCAACTTCTGCAGAGA
  CTTTATGACCCCGTGCAAGGACAAGTGCTGTTTGATGGTGTGGATGCAAAAGAATTGAAT
  GTACAGTGGCTCCGTTCCCAAATAGCAATCGTTCCTCAAGAGCCTGTGCTCTTCAACTGC
  AGCATTGCTGAGAACATCGCCTATGGTGACAACAGCCGTGTGGTGCCATTAGATGAGATC
  AAAGAAGCCGCAAATGCAGCAAATATCCATTCTTTTATTGAAGGTCTCCCTGAGAAATAC
  AACACACAAGTTGGACTGAAAGGAGCACAGCTTTCTGGCGGCCAGAAACAAAGACTAGCT
  ATTGCAAGGGCTCTTCTCCAAAAACCCAAAATTTTATTGTTGGATGAGGCCACTTCAGCC
  CTCGATAATGACAGTGAGAAGGTGGTTCAGCATGCCCTTGATAAAGCCAGGACGGGAAGG
  ACATGCCTAGTGGTCACTCACAGGCTCTCTGCAATTCAGAACGCAGATTTGATAGTGGTT
  CTGCACAATGGAAAGATAAAGGAACAAGGAACTCATCAAGAGCTCCTGAGAAATCGAGAC
  ATATATTTTAAGTTAGTGAATGCACAGTCAGTGCAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_178559
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_178559.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178559.3, NP_848654.2
• RefSeq Size: 2906 bp
• RefSeq ORF: 2439 bp
• Locus ID: 340273
• UniProt ID: Q2M3G0
• Cytogenetics: 7p21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to variant 1. This variant (2) is the mRNA isoform beta as described in PMID 15760339.