TNF alpha (TNF) (NM_000594) Human Untagged Clone

CAT#: SC125235

TNF (untagged)-Human tumor necrosis factor (TNF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNF alpha
• Synonyms: DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125235 sequence for NM_000594 edited (data generated by NextGen Sequencing)
  ATGAGCACTGAAAGCATGATCCGGGACGTGGAGCTGGCCGAGGAGGCGCTCCCCAAGAAG
  ACAGGGGGGCCCCAGGGCTCCAGGCGGTGCTTGTTCCTCAGCCTCTTCTCCTTCCTGATC
  GTGGCAGGCGCCACCACGCTCTTCTGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGG
  GAAGAGTTCCCCAGGGACCTCTCTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCT
  TCTCGAACCCCGAGTGACAAGCCTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAGGGG
  CAGCTCCAGTGGCTGAACCGCCGGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGA
  GATAACCAGCTGGTGGTGCCATCAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTC
  AAGGGCCAAGGCTGCCCCTCCACCCATGTGCTCCTCACCCACACCATCAGCCGCATCGCC
  GTCTCCTACCAGACCAAGGTCAACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAG
  ACCCCAGAGGGGGCTGAGGCCAAGCCCTGGTATGAGCCCATCTATCTGGGAGGGGTCTTC
  CAGCTGGAGAAGGGTGACCGACTCAGCGCTGAGATCAATCGGCCCGACTATCTCGACTTT
  GCCGAGTCTGGGCAGGTCTACTTTGGGATCATTGCCCTGTGA
  
  Clone variation with respect to NM_000594.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000594 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGAAAGGACACCATGAGCACTGAAAGCATGATCC
  GGGACGTGGAGCTGGCCGAGGAGGCGCTCCCCAAGAAGACAGGGGGGCCCCAGGGCTCCA
  GGCGGTGCTTGTTCCTCAGCCTCTTCTCCTTCCTGATCGTGGCAGGCGCCACCACGCTCT
  TCTGCCTGCTGCACTTTGGAGTGATCGGCCCCCAGAGGGAAGAGTTCCCCAGGGACCTCT
  CTCTAATCAGCCCTCTGGCCCAGGCAGTCAGATCATCTTCTCGAACCCCGAGTGACAAGC
  CTGTAGCCCATGTTGTAGCAAACCCTCAAGCTGAGGGGCAGCTCCAGTGGCTGAACCGCC
  GGGCCAATGCCCTCCTGGCCAATGGCGTGGAGCTGAGAGATAACCAGCTGGTGGTGCCAT
  CAGAGGGCCTGTACCTCATCTACTCCCAGGTCCTCTTCAAGGGCCAAGGCTGCCCCTCCA
  CCCATGTGCTCCTCACCCACACCATCAGCCGCATCGCCGTCTCCTACCAGACCAAGGTCA
  ACCTCCTCTCTGCCATCAAGAGCCCCTGCCAGAGGGAGACCCCAGAGGGGCTGAGGCCAG
  CCCTGTATGAGCCCATCTTCTTGAAGGGTCTTCGCTTGAGAGGTGACCGATTAACCTGAA
  TCAATGGCCGAATATCTGACTTTCCAGCTTGGCAGGTTAATTGGATCATTGCTGGAAGCC
  CCCTTATTGGGCCGGGCATATTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000594 unedited
  GCGAGCACTGGGGNAGTGGGTCACAGGGCATGCCACCCGGGTATCTGTTGCAGGAAAACA
  GCTATGACCGCGGCCGCAATCTAGAGTCGAGCCTCACAGGGCAATGATCCCAAAGTAGAC
  CTGCCCAGACTCGGCAAAGTCGAGATAGTCGGGCCGATTGATCTCAGCGCTGAGTCGGTC
  ACCCTTCTCCAGCTGGAAGACCCCTCCCAGATAGATGGGCTCATACCAGGGCTTGGCCTC
  AGCCCCCTCTGGGGTCTCCCTCTGGCAGGGGCTCTTGATGGCAGAGAGGAGGTTGACCTT
  GGTCTGGTAGGAGACGGCGATGCGGCTGATGGTGTGGGTGAGGAGCACATGGGTGGAGGG
  GCAGCCTTGGCCCTTGAAGAGGACCTGGGAGTAGATGAGGTACAGGCCCTCTGATGGCAC
  CACCAGCTGGTTATCTCTCAGCTCCACGCCATTGGCCAGGAGGGCATTGGCCCGGCGGTT
  CAGCCACTGGAGCTGCCCCTCAGCTTGAGGGTTTGCTACAACATGGGCTACAGGCTTGTC
  ACTCGGNGTTCGAGAAGATGATCTGACTGCCTGGGCCAGAGGGCTGATTAGAGAGAGGTC
  CCTGGGGAACTCTTCCCTCTGGGGGCCGATCACTCCANAGTGCAGCAGGCAGAAGAGCGT
  GGGTGGCGCCTGCCACGATCAGGGAAGAGAAGAGGCTGAGGAAACAGCACCGCCTGGAGC
  CCTGGGGCCCCCCTGTCTTCTTGGGGGAGCGCCTCTCGCCAGCTCCACGTCCCGGGATCA
  TGCTTNCAGTGCTCATGGTGGTCCTTTCGATTTCGGGGCGCCCTATGNTGAGTCGTATTA
  CAAAATTCTGACGGTTCACTAAACGAGCTTTGCTTATTTAGACCTCCCACCGTACACGC
  
• Restriction Sites: Please inquire
• ACCN: NM_000594
• Insert Size: 700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000594.2, NP_000585.2
• RefSeq Size: 1669 bp
• RefSeq ORF: 702 bp
• Locus ID: 7124
• UniProt ID: P01375
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus
• Gene Summary: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]