Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (NM_000746) Human Untagged Clone

CAT#: SC124074

CHRNA7 (untagged)-Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Nicotinic Acetylcholine Receptor alpha 7
• Synonyms: CHRNA7-2; NACHRA7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000746, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCTGCTCGCCGGGAGGCGTCTGGCTGGCGCTGGCCGCGTCGCTCCTGCACGTGTCCCTGCAAGGCG
  AGTTCCAGAGGAAGCTTTACAAGGAGCTGGTCAAGAACTACAATCCCTTGGAGAGGCCCGTGGCCAATGA
  CTCGCAACCACTCACCGTCTACTTCTCCCTGAGCCTCCTGCAGATCATGGACGTGGATGAGAAGAACCAA
  GTTTTAACCACCAACATTTGGCTGCAAATGTCTTGGACAGATCACTATTTACAGTGGAATGTGTCAGAAT
  ATCCAGGGGTGAAGACTGTTCGTTTCCCAGATGGCCAGATTTGGAAACCAGACATTCTTCTCTATAACAG
  TGCTGATGAGCGCTTTGACGCCACATTCCACACTAACGTGTTGGTGAATTCTTCTGGGCATTGCCAGTAC
  CTGCCTCCAGGCATATTCAAGAGTTCCTGCTACATCGATGTACGCTGGTTTCCCTTTGATGTGCAGCACT
  GCAAACTGAAGTTTGGGTCCTGGTCTTACGGAGGCTGGTCCTTGGATCTGCAGATGCAGGAGGCAGATAT
  CAGTGGCTATATCCCCAATGGAGAATGGGACCTAGTGGGAATCCCCGGCAAGAGGAGTGAAAGGTTCTAT
  GAGTGCTGCAAAGAGCCCTACCCCGATGTCACCTTCACAGTGACCATGCGCCGCAGGACGCTCTACTATG
  GCCTCAACCTGCTGATCCCCTGTGTGCTCATCTCCGCCCTCGCCCTGCTGGTGTTCCTGCTTCCTGCAGA
  TTCCGGGGAGAAGATTTCCCTGGGGATAACAGTCTTACTCTCTCTTACCGTCTTCATGCTGCTCGTGGCT
  GAGATCATGCCCGCAACATCCGATTCGGTACCATTGATAGCCCAGTACTTCGCCAGCACCATGATCATCG
  TGGGCCTCTCGGTGGTGGTGACAGTGATCGTGCTGCAGTACCACCACCACGACCCCGACGGGGGCAAGAT
  GCCCAAGTGGACCAGAGTCATCCTTCTGAACTGGTGCGCGTGGTTCCTGCGAATGAAGAGGCCCGGGGAG
  GACAAGGTGCGCCCGGCCTGCCAGCACAAGCAGCGGCGCTGCAGCCTGGCCAGTGTGGAGATGAGCGCCG
  TGGCGCCGCCGCCCGCCAGCAACGGGAACCTGCTGTACATCGGCTTCCGCGGCCTGGACGGCGTGCACTG
  TGTCCCGACCCCCGACTCTGGGGTAGTGTGTGGCCGCATGGCCTGCTCCCCCACGCACGATGAGCACCTC
  CTGCACGGCGGGCAACCCCCCGAGGGGGACCCGGACTTGGCCAAGATCCTGGAGGAGGTCCGCTACATTG
  CCAACCGCTTCCGCTGCCAGGACGAAAGCGAGGCGGTCTGCAGCGAGTGGAAGTTCGCCGCCTGTGTGGT
  GGACCGCCTGTGCCTCATGGCCTTCTCGGTCTTCACCATCATCTGCACCATCGGCATCCTGATGTCGGCT
  CCCAACTTCGTGGAGGCCGTGTCCAAAGACTTTGCGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000746 unedited
  TAAAGTCAGATTTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCGA
  GGTGCCTCTGTGGCCGCAGGCGCAGGCCCGGGCGACAGCCGAGACGTGGAGCGCGCCGGC
  TCGCTGCAGCTCCGGGACTCAACATGCGCTGCTCGCCGGGAGGCGTCTGGCTGGCGCTGG
  CCGCGTCGCTCCTGCACGTGTCCCTGCAAGGCGAGTTCCAGAGGAAGCTTTACAAGGAGC
  TGGTCAAGAACTACAATCCCTTGGAGAGGCCCGTGGCCAATGACTCGCAACCACTCACCG
  TCTACTTCTCCCTGAGCCTCCTGCAGATCATGGACGTGGATGAAAAGAACCAAGTTTTAA
  CCACCAACATTTGGCTGCAAATGTCTTGGACAGATCACTATTTACAGTGGAATGTGTCAT
  AATATCCAGGGGTGAAGACTGTTCGTTTCCCAGATGGCCACATTTGGAAACCAGACATTC
  TTCTCTATAACAGTGCTGATGAGCGCTTTGACGCCACATTCCACACTAACGTGTTGGTGA
  ATTCTTCTGGGCATTGCCAGTACCTGCCTCCAGGCATATTCAAGAGTTCCTGCTACATCG
  ATGTACGCTGGTTTCCCTTTGATGTGCAGCACTGCATACTGAAGTTTGGGTCCTGGTCTT
  ACGGACGCTGGCCCTTGGATCTGCAGATGCAGGGAGCAGATATCACTGGCTATATCCGCA
  AGGGAAAATGGGACCTAGTGGGAATCCCCCGCAAGAGGAGTGAAAGGTTATATGAGTGCT
  GCAAAGAACCCTACCCCGATGTCAACTTTACAGTGACCATGCGCAAAAGGACGCTCCACT
  ATTGGGCTCAACGGCTGATCCCCTGGGGGAAAAATTTCCACCCTGCGCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000746 unedited
  CATGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT
  GTTTTTTTTAACGGAGAAACAGCATCAAGCTGTTTCTCTCTACCGTCTTTGATAGAAATA
  AAAATAAAAATAAAAAGTTGAATTGCAGAAAAGCTAAGAGGTTTTTAGTTTTTGTTTTTT
  GTTTTCCTTCCACCAGTCAATTATTGGAAAGGATTTAGTGAGTCTGGTTTATTTTAGCTT
  CAATCTGGGTTTGTACACAAGCAAAAAGCAAATGTTGAATTTTCAAGTAGACCTTCATGC
  AGACATGCAAAACCAACTGTCTCGGTGGTGAGGAGCCATGGGGAGCTCTCCGAAGGGCTT
  TCCAAGCAGTGGGCTAATGGGCAAAATGACTACTCAATGGCCCTGCTTACCGATGGTACT
  GATGTGCCAAGGAATATCTATCAGCCCATCCGAAAATATTGAAACAAATGCTTGAGTTTC
  TCTTCCCTAAAGTAGCAAAGATACCGTAGGCACCACGACTTGACAGCCAGATGGGACCAC
  TTGAGTTGAGGCGTGTAATGCTGTCCTGTATTAGCACCCCCAAATCTCGTCAAGCCCAAA
  GGCCTTGCCCATCTGTGATCTTCCACATGTACAGAACCCAGCGTAGTTACGCCAAGTCTT
  TTGACACGGCCTTCACGAAGTGGCAGCCGACTACNTGTTGCCAATGGTGCCAATTAGGGT
  AAGAACCGGAAGGCTTAAGGGAAGGCGTTCTCCCACGGCGGAGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_000746
• Insert Size: 4900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000746.2, NP_000737.1
• RefSeq Size: 2155 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1139
• UniProt ID: P36544
• Cytogenetics: 15q13.3
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) lacks an in-frame portion of the 5' coding region, compared to variant 2. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.