HLA (BC012106) Human Untagged Clone

CAT#: SC123658

HLA (untagged)-Human major histocompatibility complex, class II, DQ beta 1 (cDNA clone MGC:20093 IMAGE:4574913), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA
• Synonyms: CELIAC1; HLA-DQB; IDDM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC012106 edited
  ATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATTATGTCT
  TGGAAGAAGGCTTTGCGGATCCCTGGAGGCCTTCGGGTAGCAACTGTGACCTTGATGCTG
  GCGATGCTGAGCACCCCGGTGGCTGAGGGCAGAGACTCTCCCGAGGATTTCGTGTACCAG
  TTTAAGGGCATGTGCTACTTCACCAACGGGACGGAGCGCGTGCGTCTTGTGACCAGATAC
  ATCTATAACCGAGAGGAGTACGCACGCTTCGACAGCGACGTGGGGGTGTATCGGGCGGTG
  ACGCCGCTGGGGCCGCCTGCCGCCGAGTACTGGAACAGCCAGAAGGAAGTCCTGGAGAGG
  ACCCGGGCGGAGTTGGACACGGTGTGCAGACACAACTACCAGTTGGAGCTCCGCACGACC
  TTGCAGCGGCGAGTGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCCCTCAAC
  CACCACAACCTGCTGGTCTGCTCAGTGACAGATTTCTATCCAGCCCAGATCAAAGTCCGG
  TGGTTTCGGAATGACCAGGAGGAGACAACTGGCGTTGTGTCCACCCCCCTTATTAGGAAC
  GGTGACTGGACCTTCCAGATCCTGGTGATGCTGGAAATGACTCCCCAGCGTGGAGACGTC
  TACACCTGCCACGTGGAGCACCCCAGCCTCCAGAACCCCATCATCGTGGAGTGGCGGGCT
  CAGTCTGAATCTGCCCAGAGCAAGATGCTGAGTGGCATTGGAGGCTTCGTGCTGGGGCTG
  ATCTTCCTCGGGCTGGGCCTTATTATCCATCACAGGAGTCAGAAAGGGCTCCTGCACTGA
  CTCCTGAGACTATTTTAACTGGGATTGGTTATCACTTTTCTGTAACGCCTGCTTGTCCCT
  GCCCAGAATTCCCAGCTGCCTGTGTCAGCCTGTCCCCCTGAGATCAGAGTCCTACAGTGG
  CTGTCACGCAGCCACCAGGTCATCTCCTTTCATCCCCACCTCGAGGCTGATGGCTGTGAC
  CCTGCTTCCTGCACTTACCCAGAGCCTCTGCCTGTGCACGGCCAGCTGCGTCTACTGAGG
  CCCCAAGGGGTTTCTGTTTCTATTCTCTCCTCAGACTGCTCAAGAGAAGCACATGAAAAC
  CAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC012106 unedited
  NATCTTTGGGTTTTGTAATACGACTTACTATAGGGCGGCACGCGAATTCGCACGAGGATC
  CATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCAATTATGTCTTGG
  AAGAAGGCTTTGCGGATCCCTGGAGGCCTTCGGGTAGCAACTGTGACCTTGATGCTGGCG
  ATGCTGAGCACCCCGGTGGCTGAGGGCAGAGACTCTCCCGAGGATTTCGTGTACCAGTTT
  AAGGGCATGTGCTACTTCACCAACGGGACGGAGCGCGTGCGTCTTGTGACCAGATACATC
  TATAACCGAGAGGAGTACGCACGCTTCGACAGCGACGTGGGGGTGTATCGGGCGGTGACG
  CCGCTGGGGCCGCCTGCCGCCGAGTACTGGAACAGCCAGAAGGAAGTCCTGGAGAGGACC
  CGGGCGGAGTTGGACACGGTGTGCAGACACAACTACCAGTTGGAGCTCCGCACGACCTTG
  CAGCGGCGAGTGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCCCTCAACCAC
  CACAACCTGCTGGTCTGCTCAGTGACAGATTTCTATCCAGCCCAGATCAAAGTCCGGTGG
  TTTCGGAATGACCAGGAGGAGACAACTGGCGTTGTGTCCACCCCCCTTATTAGGAACGGT
  GACTGGACCTTCCAGATCCTGGTGATGCTGGAAATGACTCCCCAGCGTGGAGACGTCTAC
  ACCTGCCACGTGGAGCACCCCAGCCTCCAGAACCCCATCATCGTGGAGTGGCGGGCTCAG
  TCTGAATCTGCCCAGAGCAGATGCTGAGTGGCATTGGAGGCTTCGTGCNTGGGCTGATCT
  TCCTCCGGCTGGGNCTTATTATNCATCACAGGAGTCAGAAAGGGCTNCTGCACTGACTCC
  TG
  
• Restriction Sites: Please inquire
• ACCN: BC012106
• Insert Size: 1160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012106.1, AAH12106.1
• RefSeq Size: 1160 bp
• RefSeq ORF: 783 bp
• Locus ID: 3119
• Cytogenetics: 6p21.32
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]