PLA2G2D (NM_012400) Human Untagged Clone

CAT#: SC122785

PLA2G2D (untagged)-Human phospholipase A2, group IID (PLA2G2D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2G2D
• Synonyms: PLA2IID; sPLA2-IID; sPLA2S; SPLASH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012400 edited
  TGCTCTGTGCTGGGATCATGGAACTTGCACTGCTGTGTGGGCTGGTGGTGATGGCTGGTG
  TGATTCCAATCCAGGGCGGGATCCTGAACCTGAACAAGATGGTCAAGCAAGTGACTGGGA
  AAATGCCCATCCTCTCCTACTGGCCCTACGGCTGTCACTGCGGACTAGGTGGCAGAGGCC
  AACCCAAAGATGCCACGGACTGGTGCTGCCAGACCCATGACTGCTGCTATGACCACCTGA
  AGACCCAGGGGTGCAGCATCTACAAGGACTATTACAGATACAACTTTTCCCAGGGGAACA
  TCCACTGCTCTGACAAGGGAAGCTGGTGTGAGCAGCAGCTGTGTGCCTGTGACAAGGAGG
  TGGCCTTCTGCCTGAAGCGCAACCTGGACACCTACCAGAAGCGACTGCGTTTCTACTGGC
  GGCCCCACTGCCGGGGGCAGACCCCTGGGTGCTAGAAGCCCACACCCTCTACCCTGTTCC
  TCAGCATGGAGCTCTGGCATCCCCACCTCAGTATCTAACCTGAACCAGCCTGGCTTTTCA
  AACACTCCGGGGGGAGGTAGTCCCAGCCTCCCCCGGAACCCTCTACCAATGCCTTCTGAC
  CTTCTGAAGCTTTCCGAATCCTCCCAGTTGAGGCAGTAGCTGTGTCCTCTGAGGGTGGAT
  GGGAATCTTGGGAGAAGCCCAAGCAAGGGAGCCCTCAGAGGTGGTGTTTGGACCAAAGCA
  TCGGGGTGGGGGAGGGGTCTGCCGCTGTCCCCCACCTGCTGGCCCCCTTGTCCTTCCTCA
  CCCCCTCCAATATAGTCTCGGAGCTACAACCGCAGCAGCCACTATAAAGGGCAATATTGA
  TCTTTCTGTCCATGTGGCTCTATCTTTTAAAACCTCAAGGCCCTCCACTGTCCTAAGATA
  AAGCCTCTCATAGGCACTGGGGACCCTGCACAGTCTGGCCATGTGACCCTCTCCCCAGGC
  AAGCTCTGAAGTCCCTGCAGGTGGAGGCCATGCCTGTCTTAAACTCAGTTGCATCCCTGG
  TGCCCAAAGCAACACCAGAACCAAGAAGGAGCTCCATAAATCCTTCTTGGGTGAAGCCTA
  GACAAAGCCGCCAGGTCTTGTGGCTCCAGGCACCAGAGCCTTGAGTACTTTCTCCTGCCT
  CCAGGCATTGGCTCAGGGTGAATTACAAGGGGCTACTGAATGGCTATTACTTTCATCACG
  ACTGATCCCCACCTCCTCAGGGTCAAAGGGCTACTTTCTGGAAGTCTCCCCAGGCTGACT
  CCTTCTCCCTGACTGCAAGGGCTCACTCCCTCCTCCAAGCTCCCACAATGCTTCATGGCT
  CTGCCGCTTACCTAGCTTGGCCTAGAGTGGCAAATGGAACTTCTCTGATCTCCCCCAACT
  AGACTGGAGCCCCCGAAGGATGGAGACCATGTCTGTGCCATCTCTGTTTCCCCTGTTTTC
  CCACATACTAGGTGCTCAATTCATGCCTGTGAATGGCGTGAGCCCATAATGGATACACAG
  AGGTTGCAGCAGATGGTGTGGGTACCTCACCCAGATATCATCCAGGCCCAAGGCCCCTCT
  CCCTGAGTGAGGCCAGGTGTTGGCAGCCAACTGCTCCAATCTGCCTCCTTCCCCTAAATA
  CTGCCCTGGTCTAGTGGGAGCTGCCTTCCCCCTGCCCCACCTCTCCCACCAAGAGGCCAC
  CTGTCACTCATGGCCAGGAGAGTGACACCATGGAGGGTACAATTGCCAGCTCCCCCGTGT
  CTGTGCAGGATTGTCTGGGTTGAATGACACTCTCAAATTGTTCCTGGGATCGGGCTGAGG
  CCAGGCCTCTCCTGGAACCACCTCTCTGCTTGGTCTGACCCCTTGGCCTATCCAGTTTTC
  CTGGTTCCCTCACAGGTTTCTCCAGAAAGTACTCCCTCAGTAAAGCATTTGCACAAGAAT
  CCTTGTCTCAGGCTCTGCTTCTAAGGAAAGAGACTGAAGAGGGACAACTTTTTCCTATGA
  GGACTCCCAATCTGCCATTTGTGTGTGGCTAGCTGTTTCCAGCCAGGGGGCTGAGGCACC
  TGCAGTCACCCACACTCTGGGCCTGTGGCACCATCTGTGCCTCAGCTGACTGAGCAGTTC
  TGAGATGTGGTGACTCATGTGAAGTGGTGTCATAGGAATGAAGGACTGTGGGCGCTGGGT
  TCAAATTCCAGTTCTATGCACCACTCCAGGTAGGGATACCCATGGGGGCAGCTGTGCCTG
  TGTGGGGGAACAGGGTAGGGAAATCTCCATACCTTCTCAATTTTGCTGTGAACCTAAAAC
  TGCCATATAAAGATAAAGTCTAGGCCAGGCATGGTGGCTCATGCCTGTAATCCCAACACT
  TAGGGTGGCTGAGGCAGGCAGATCACCTGGGGTCAGGAGTTCAAGACCAGCCTGCCCAAC
  ATGGCAAAACCTCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATCGTGGTGGGTGCC
  TGTAATCCCAGCTATTCAGGAGGCTGAGGCAGGAGAATCACTTGGACCTGGGAGGCAGAG
  GTTTCAGTGAGCCCAGATCGCGACACTGCACTCCAGCCTGGGCAACAGAGTGACACTCTG
  TCTCAAAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012400 unedited
  GTTGCATTGTAAACACTTTTATAGGCGGCCGCGATTCCCGGNTTGCTCTGTGCTGTGATC
  ATGGAACTTGCACTGCTGTGTGGGCTGGTGGTGATGGCTGGTGTGATTCCAATCCAGGGC
  GGGATCCTGAACCTGAACAAGATGGTCAAGCAAGTGACTGGGAAAATGCCCATCCTCTCC
  TACTGGCCCTACGGCTGTCACTGCGGACTATGTGGCAGAGGCCAACCCAAAGATGCCACG
  GACTGGTGCTGCCAGACCCATGACTGCTGCTATGACCACCTGAAGACCCAGGGGTGCAGC
  ATCTACAAGGACTATTACAGATACAACTTTTCCCAGGGGAACATCCACTGCTCTGACAAG
  GGAAGCTGGTGTGAGCAGCAGCTGTGTGCCTGTGACAAGGAGGTGGCCTTCTGCCTGAAG
  CGCAACCTGGACACCTACCAGAAGCGACTGCGTTTCTACTGGCGGCCCCACTGCCGGGGG
  CAGACCCCTGAGTGCTAGAAGCCCACACCCTCTACCCTGTTCCTCAGCATGGAGCTCTAG
  CATCCCCACCTCAGTATCTAACCTGAACCAGCCTGGCTTTTCAAACACTCCGGGGGGAGG
  TAGTCCCAGCCTCCCCCGGAACCTCTACCAATGCCCTCTGACCTTCTGAAGCTTTCCAAA
  TCCTCCCAGTTGGAGCAGTAGCTGTGTCCTCTGAGGTTGGTGGGGAATCTTGGGAGACCC
  CAAGCCAGGGAGCCCTCCAGAGGTGGTGTTTGGACCAAAGCTTCGGGTGGGGGAGAGGGT
  CTGCCCTGTTCCCCACTGTTTGGCCCCTTTGGCTTCCTAACCCCTCCAATTATTCCCGGA
  GCTACATCCGGCAAGCCACTATAAAGGCAAATTTGATCTTTCTTGTCCTGGGCTCCATTC
  TTTTAAACCCTAGAGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_012400
• Insert Size: 2747 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012400.2, NP_036532.1
• RefSeq Size: 2747 bp
• RefSeq ORF: 438 bp
• Locus ID: 26279
• UniProt ID: Q9UNK4
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).