Fc epsilon RI (FCER1A) (NM_002001) Human Untagged Clone

CAT#: SC122614

FCER1A (untagged)-Human Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide (FCER1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fc epsilon RI
• Synonyms: FCE1A; FcERI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002001 edited
  ACAGTAAGCACCAGGAGTCCATGAAGAAGATGGCTCCTGCCATGGAATCCCCTACTCTAC
  TGTGTGTAGCCTTACTGTTCTTCGCTCCAGATGGCGTGTTAGCAGTCCCTCAGAAACCTA
  AGGTCTCCTTGAACCCTCCATGGAATAGAATATTTAAAGGAGAGAATGTGACTCTTACAT
  GTAATGGGAACAATTTCTTTGAAGTCAGTTCCACCAAATGGTTCCACAATGGCAGCCTTT
  CAGAAGAGACAAATTCAAGTTTGAATATTGTGAATGCCAAATTTGAAGACAGTGGAGAAT
  ACAAATGTCAGCACCAACAAGTTAATGAGAGTGAACCTGTGTACCTGGAAGTCTTCAGTG
  ACTGGCTGCTCCTTCAGGCCTCTGCTGAGGTGGTGATGGAGGGCCAGCCCCTCTTCCTCA
  GGTGCCATGGTTGGAGGAACTGGGATGTGTACAAGGTGATCTATTATAAGGATGGTGAAG
  CTCTCAAGTACTGGTATGAGAACCACAACATCTCCATTACAAATGCCACAGTTGAAGACA
  GTGGAACCTACTACTGTACGGGCAAAGTGTGGCAGCTGGACTATGAGTCTGAGCCCCTCA
  ACATTACTGTAATAAAAGCTCCGCGTGAGAAGTACTGGCTACAATTTTTTATCCCATTGT
  TGGTGGTGATTCTGTTTGCTGTGGACACAGGATTATTTATCTCAACTCAGCAGCAGGTCA
  CATTTCTCTTGAAGATTAAGAGAACCAGGAAAGGCTTCAGACTTCTGAACCCACATCCTA
  AGCCAAACCCCAAAAACAACTGATATAATTACTCAAGAAATATTTGCAACATTAGTTTTT
  TTCCAGCATCAGCAATTGCTACTCAATTGTCAAACACAGCTTGCAATATACATAGAAACG
  TCTGTGCTCAAGGATTTATAGAAATGCTTCATTAAACTGAGTGAAACTGGTTAAGTGGCA
  TGTAATAGTAAGTGCTCAATTAACATTGGTTGAATAAATGAGAGAATGAATAGATTCATT
  TATTAGCATTTGTAAAAGAGATGTTCAATTTCAATAAAATAAATATAAAACCAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002001 unedited
  NGACACGTTTACATTTGTAAACGACTCCTATAGGCGGCCGCGNAATTCGCCATTACGGCC
  GGGGACAGTAAGCACCAGGAGTCCATGAAGAAGAGGCTCCTGCCATGGAATCCCCTACTC
  TACTGTGTGTAGCCTTACTGTTCTTCGCTCCAGATGGCGTGTTAGCAGTCCCTCAGAAAC
  CTAAGGTCTCCTTGAACCCTCCATGGAATAGAATATTTAAAGGAGAGAATGTGACTCTTA
  CATGTAATGGGAACAATTTCTTTGAAGTCAGTTCCACCAAATGGTTCCACAATGGCAGCC
  TTTCAGAAGAGACAAATTCAAGTTTGAATATTGTGAATGCCAAATTTGAAGACAGTGGAG
  AATACAAATGTCAGCACCAACAAGTTAATGAGAGTGAACCTGTGTACCTGGAAGTCTTCA
  GTGACTGGCTGCTCCTTCAAGCCTCTGCTGAGGTGGTGATGGAGGGCCAGCCCCTCTTCC
  TCAGGTGCCATGGTTGGAGGAACTGGGATGTGTACAAAGTGATCTATTATAAGGATGGTG
  AAGCTCTCAAGTACTGGTATGAGAACCACAACATCTCCATTACAAATGCCACAGTTGAAG
  ACAGTGGAACCTACTACTGTACGGGCAAAGTGTGGCAGCTGGACTATGAGTCTGAGCCCC
  TCAACATTACTGTAATAAAAGCTCCGCGTGAGAAGTACTGGCTACAATTTTTTATCCCAT
  TGTTGGTGGTGATTCTGTTTGCTGTGGACACAGGATTATTTATCTCAACTCAGCAGCAAG
  TCACATTTCTCTTGAAGATTAAGAGAACAGGAAAGGCTTCAGACTTCTGAACCCCACATC
  CTAGCCAAACCCCAAAACCACTG
  
• Restriction Sites: Please inquire
• ACCN: NM_002001
• Insert Size: 1102 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002001.2, NP_001992.1
• RefSeq Size: 1191 bp
• RefSeq ORF: 774 bp
• Locus ID: 2205
• UniProt ID: P12319
• Cytogenetics: 1q23.2
• Protein Families: Transmembrane
• Protein Pathways: Asthma, Fc epsilon RI signaling pathway
• Gene Summary: The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]