TAP1 (NM_000593) Human Untagged Clone

CAT#: SC122568

TAP1 (untagged)-Human transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) (TAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAP1
• Synonyms: ABC17; ABCB2; APT1; D6S114E; PSF-1; PSF1; RING4; TAP1*0102N; TAP1N
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000593 edited
  GTGTGCGTGATGGAGAAAATTGGGCACCAGGGCTGCTCCCGAGATTCTCAGATCTGATTT
  CCACGCTTGCTACCAAAATAGTCTGGGCAGGCCACTTTTGGAAGTAGGCGTTATCTAGTG
  AGCAGGCGGCCGCTTTCGATTTCGCTTTCCCCTAAATGGCTGAGCTTCTCGCCAGCGCAG
  GATCAGCCTGTTCCTGGGACTTTCCGAGAGCCCCGCCCTCGTTCCCTCCCCCAGCCGCCA
  GTAGGGGAGGACTCGGCGGTACCCGGAGCTTCAGGCCCCACCGGGGCGCGGAGAGTCCCA
  GGCCCGGCCGGGACCGGGACGGCGTCCGAGTGCCAATGGCTAGCTCTAGGTGTCCCGCTC
  CCCGCGGGTGCCGCTGCCTCCCCGGAGCTTCTCTCGCATGGCTGGGGACAGTACTGCTAC
  TTCTCGCCGACTGGGTGCTGCTCCGGACCGCGCTGCCCCGCATATTCTCCCTGCTGGTGC
  CCACCGCGCTGCCACTGCTCCGGGTCTGGGCGGTGGGCCTGAGCCGCTGGGCCGTGCTCT
  GGCTGGGGGCCTGCGGGGTCCTCAGGGCAACGGTTGGCTCCAAGAGCGAAAACGCAGGTG
  CCCAGGGCTGGCTGGCTGCTTTGAAGCCATTAGCTGCGGCACTGGGCTTGGCCCTGCCGG
  GACTTGCCTTGTTCCGAGAGCTGATCTCATGGGGAGCCCCCGGGTCCGCGGATAGCACCA
  GGCTACTGCACTGGGGAAGTCACCCTACCGCCTTCGTTGTCAGTTATGCAGCGGCACTGC
  CCGCAGCAGCCCTGTGGCACAAACTCGGGAGCCTCTGGGTGCCCGGCGGTCAGGGCGGCT
  CTGGAAACCCTGTGCGTCGGCTTCTAGGCTGCCTGGGCTCGGAGACGCGCCGCCTCTCGC
  TGTTCCTGGTCCTGGTGGTCCTCTCCTCTCTTGGGGAGATGGCCATTCCATTCTTTACGG
  GCCGCCTCACTGACTGGATTCTACAAGATGGCTCAGCCGATACCTTCACTCGAAACTTAA
  CTCTCATGTCCATTCTCACCATAGCCAGTGCAGTGCTGGAGTTCGTGGGTGACGGGATCT
  ATAACAACACCATGGGCCACGTGCACAGCCACTTGCAGGGAGAGGTGTTTGGGGCTGTCC
  TGCGCCAGGAGACGGAGTTTTTCCAACAGAACCAGACAGGTAACATCATGTCTCGGGTAA
  CAGAGGACACGTCCACCCTGAGTGATTCTCTGAGTGAGAATCTGAGCTTATTTCTGTGGT
  ACCTGGTGCGAGGCCTATGTCTCTTGGGGATCATGCTCTGGGGATCAGTGTCCCTCACCA
  TGGTCACCCTGATCACCCTGCCTCTGCTTTTCCTTCTGCCCAAGAAGGTGGGAAAATGGT
  ACCAGTTGCTGGAAGTGCAGGTGCGGGAATCTCTGGCAAAGTCCAGCCAGGTGGCCATTG
  AGGCTCTGTCGGCCATGCCTACAGTTCGAAGCTTTGCCAACGAGGAGGGCGAAGCCCAGA
  AGTTTAGGGAAAAGCTGCAAGAAATAAAGACACTCAACCAGAAGGAGGCTGTGGCCTATG
  CAGTCAACTCCTGGACCACTAGTATTTCAGGTATGCTGCTGAAAGTGGGAATCCTCTACA
  TTGGTGGGCAGCTGGTGACCAGTGGGGCTGTAAGCAGTGGGAACCTTGTCACATTTGTTC
  TCTACCAGATGCAGTTCACCCAGGCTGTGGAGGTACTGCTCTCCATCTACCCCAGAGTAC
  AGAAGGCTGTGGGCTCCTCAGAGAAAATATTTGAGTACCTGGACCGCACCCCTCGCTGCC
  CACCCAGTGGTCTGTTGACTCCCTTACACTTGGAGGGCCTTGTCCAGTTCCAAGATGTCT
  CCTTTGCCTACCCAAACCGCCCAGATGTCTTAGTGCTACAGGGGCTGACATTCACCCTAC
  GCCCTGGCGAGGTGACGGCGCTGGTGGGACCCAATGGGTCTGGGAAGAGCACAGTGGCTG
  CCCTGCTGCAGAATCTGTACCAGCCCACCGGGGGACAGCTGCTGTTGGATGGGAAGCCCC
  TTCCCCAATATGAGCACCGCTACCTGCACAGGCAGGTGGCTGCAGTGGGACAAGAGCCAC
  AGGTATTTGGAAGAAGTCTTCAAGAAAATATTGCCTATGGCCTGACCCAGAAGCCAACTA
  TGGAGGAAATCACAGCTGCTGCAGTAAAGTCTGGGGCCCATAGTTTCATCTCTGGACTCC
  CTCAGGGCTATGACACAGAGGTAGACGAGGCTGGGAGCCAGCTGTCAGGGGGTCAGCGAC
  AGGCAGTGGCGTTGGCCCGAGCATTGATCCGGAAACCGTGTGTACTTATCCTGGATGATG
  CCACCAGTGCCCTGGATGCAAACAGCCAGTTACAGGTGGAGCAGCTCCTGTACGAAAGCC
  CTGAGCGGTACTCCCGCTCAGTGCTTCTCATCACCCAGCACCTCAGCCTGGTGGAGCAGG
  CTGACCACATCCTCTTTCTGGAAGGAGGCGCTATCCGGGAGGGGGGAACCCACCAGCAGC
  TCATGGAGAAAAAGGGGTGCTACTGGGCCATGGTGCAGGCTCCTGCAGATGCTCCAGAAT
  GAAAGCCTTCTCAGACCTGCGCACTCCATCTCCCTCCCTTTTCTTCTCTCTGTGGTGGAG
  AACCACAGCTGCAGAGTAGGCAGCTGCCTCCAGGATGAGTTACTTGAAATTTGCCTTGAG
  TGTGTTACCTCCTTTCCAAGCTCCTCGTGATAATGCAGACTTCCTGGAGTACAAACACAG
  GATTTGTAATTCCTTACTGTAACGGAGTTTAGAGCCAGGGCTGATGCTTTGGTGTGGCCA
  GCACTCTGAAACTGAGAAATGTTCAGAATGTACGGAAAGATGATCAGCTATTTTCAACAT
  AACTGAAGGCATATGCTGGCCCATAAACACCCTGTAGGTTCTTGATATTTATAATAAAAT
  TGGTGTTTTGTAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000593 unedited
  NCCGCAGTTCGGATTTGTNATACGACTTCACTATAGGCGGCCGCGCAATTCGCACGAGGT
  GTGCGTGATGGAGAAAATGGGCACCAGGGCTGCTCCCGAGATTCTCAGATCTGATTTCCA
  CGCTTGCTACCAAAATAGTCTGGGCAGGCCACTTTTGGAAGTAGGCGTTATCTATTGAGC
  AGGCGGCCGCTTTCGATTTCGCTTTCCCCTAAATGGCTGAGCTTCTCGCCAGCGCAGGAT
  CAGCCTGTTCCTGGGACTTTCCGAGAGCCCCGCCCTCGTTCCCTCCCCCAGCCGCCAGTA
  GGGGAGGACTCGGCGGTACCCGGAGCTTCAGGCCCCACCGGGGCGCGGAGAGTCCCAGGC
  CCGGCCGGGACCGGGACGGCGTCCGAGTGCCAATGGCTAGCTCTAGGTGTCCCGCTCCCC
  GCGGGTGCCGCTGCCTCCCCGGAGCTTCTCTCGCATGGCTGGGGACAGTACTGCTACTTC
  TCGCCGACTGGGTGCTGCTCCGGACCGCGCTGCCCCGCATATTCTCCCTGCTGGTGCCCA
  CCGCGCTGCCACTGCTCCGGGTCTGGGCGGTGGGCCTGAGCCGCTGGGCCGTGCTCTGGC
  TGGGGGCCTGCGGGGTCCTCAGGGCAACGGTTGGCTCCAAAAGCGAAAACGCAGGTGCCC
  AGGGCTGGCTGGCTGCTTTGAAGCCATTACCTGCGGCACTGGGGCTTGGCCCTGCCCGGG
  ACTTGCCTTGTTCCCGAGAGCTGATCTCATGGGGAGCCCCCGGGTCCCCGGAATACACCA
  GCTACTGGACTGGGGGAAATCACCCTACCCCCTCGTTGGCCGATATGCAGCGGCACTGCC
  CGCACAACCCTGTGGCCAAACCTCGGACCCCTGGGTGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_000593
• Insert Size: 2983 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000593.5, NP_000584.2
• RefSeq Size: 2974 bp
• RefSeq ORF: 2427 bp
• Locus ID: 6890
• UniProt ID: Q03518
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters, Antigen processing and presentation, Primary immunodeficiency
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).