HLA DP (HLA-DPA1) (NM_033554) Human Untagged Clone

CAT#: SC120116

HLA (untagged)-Human major histocompatibility complex, class II, DP alpha 1 (HLA-DPA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA DP
• Synonyms: DP(W3); DP(W4); DPA1; HLA-DP1A; HLA-DPA; HLA-DPB1; HLADP; HLASB; PLT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120116 sequence for NM_033554 edited (data generated by NextGen Sequencing)
  ATGCGCCCTGAAGACAGAATGTTCCATATCAGAGCTGTGATCTTGAGAGCCCTCTCCTTG
  GCTTTCCTGCTGAGTCTCCGAGGAGCTGGGGCCATCAAGGCGGACCATGTGTCAACTTAT
  GCCGCGTTTGTACAGACGCATAGACCAACAGGGGAGTTTATGTTTGAATTTGATGAAGAT
  GAGATGTTCTATGTGGATCTGGACAAGAAGGAGACCGTCTGGCATCTGGAGGAGTTTGGC
  CAAGCCTTTTCCTTTGAGGCTCAGGGCGGGCTGGCTAACATTGCTATATTGAACAACAAC
  TTGAATACCTTGATCCAGCGTTCCAACCACACTCAGGCCACCAACGATCCCCCTGAGGTG
  ACCGTGTTTCCCAAGGAGCCTGTGGAGCTGGGCCAGCCCAACACCCTCATCTGCCACATT
  GACAAGTTCTTCCCACCAGTGCTCAACGTCACGTGGCTGTGCAACGGGGAGCTGGTCACT
  GAGGGTGTCGCTGAGAGCCTCTTCCTGCCCAGAACAGATTACAGCTTCCACAAGTTCCAT
  TACCTGACCTTTGTGCCCTCAGCAGAGGACTTCTATGACTGCAGGGTGGAGCACTGGGGC
  TTGGACCAGCCGCTCCTCAAGCACTGGGAGGCCCAAGAGCCAATCCAGATGCCTGAGACA
  ACGGAGACTGTGCTCTGTGCCCTGGGCCTGGTGCTGGGCCTAGTCGGCATCATCGTGGGC
  ACCGTCCTCATCATAAAGTCTCTGCGTTCTGGCCATGACCCCCGGGCCCAGGGGACCCTG
  TGA
  
  Clone variation with respect to NM_033554.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033554 unedited
  AGCATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCTCATCAC
  TGTTCCTGTGCTCACAGTCATCAATTATAGACCCCACAACATGCGCCCTGAAGACAGAAT
  GTTCCATATCAGAGCTGTGATCTTGAGAGCCCTCTCCTTGGCTTTCCTGCTGAGTCTCCG
  AGGAGCTGGGGCCATCAAGGCGGACCATGTGTCAACTTATGCCGCGTTTGTACAGACGCA
  TAGACCAACAGGGGAGTTTATGTTTGAATTTGATGAAGATGAGATGTTCTATGTGGATCT
  GGACAAGAAGGAGACCGTCTGGCATCTGGAGGAGTTTGGCCAAGCCTTTTCCTTTGAAGC
  TCAGGGCGGGCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCAGCG
  TTCCAACCACACTCAGGCCACCAACGATCCCCCTGAGGTGACCGTGTTTCCCAAGGAGCC
  TGTGGAGCTGGGCCAGCCCAACACCCTCATCTGCCACATTGACAAGTTCTTCCCACCAGT
  GCTCAACGTCACGTGGCTGTGCAACGGGGAGCTGGTCACTGAGGGTGTCGCTGAGAGCCT
  CTTCCTGCCCAGAACAGATTACAGCTTCCACAAGTTCCATTACCTGACCTTTGTGCCCTC
  AGCAGAGGACTTCTATGACTGCAGGGTGGACCACTGGGGCTTGGACCAGCCGCTCCTCAA
  GCACTGGGAGGCCCCAGAGCCAATCCANATGCCTGAGACACCGGAAACTGTGCCCTGTGC
  CCTGTGCCTGGTGCTGGGCCCATCCGGCATCATCCTGGGCCCCGTCCCTAATATAAAAAT
  CCTGCGTTTTGGCCCTGACCCCCCGGCCCCAGGGGACCCTGTGAAAAACCGGAAAGGGGA
  CAAAAATCCCGACGCAAAAGGACTAAGAAGAAACTGAACCTCACCTGCCCACAAACTCCT
  CTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033554 unedited
  CCCTCCGTCCCNTCGCGGCCCCNGCCCCCCCTCTCCTCCCCTCGCATTTGATCTGTAGNC
  ACNCGCCGGNCCCGCCACTCCTCGATGATNCCGAGCTTCTTCTTTCTTTCTTTTCTTTCC
  CTGNAAATACTCGAAGTATCTTATCTGGTTCTCTAGGGTCATCTCTCGGGCAAGGTGACA
  GCGAGGGGTTCTTTACAGGACAACAGAGGACACCAGGCTCACTGACAGGATACTCTCCAT
  ATTAGTGCTACCACAGTGTCATTAATTTCTATTTGTCGGAAACTTTACTAAGGACTGACT
  GCTTCGAGGCAATGGATAACGACAGAGCTCGACGGGCCACTAATTCACTCAGCCACTGGA
  CTAGCTTTCACATGAAGCGAGAATAAAAGCTTGACATGGAGTTCGTACGCCCCCTGCAGC
  TTCAGATCTCTCCTAACTCCTCTTCTGCTCAGATATCTCTGAACCTTTACAGTATCCCAC
  AGCGCCCCCCTGTGCCCCGCGGTCATGGCCAGAACCCACAGACTTTATGATCAGGACGGT
  GCCCACAATCACGCCGACTACGCCCCCAGCCCCAGCCCCCGGGCACATAAAACAGTCTCC
  CTTGTCCTCCGCATCCGGATTGGCCCCTTGGGCCCCCCAGGGCTTTGAGGCACCGCCCGG
  CCCAAGACCCAACGCTTCCACCTGCAATCATACACACTCTTTGCTGACCCGAACCAACGT
  TCCGTTATCGTACCCTGTCGGAACGCTTCCCCCTGTCCCGGCCAAGACCCGATCCCCCCC
  CACCCCCTCCAGCGCCCGTTTCCCCGTTGTACCACCCACGTGACTCCCCAACCCCGCCGC
  GCCAAAACTCCCCCACGTGCTCCCACCACGCCCTCCCCCTCCCCCAACTTCCCCGCCTTT
  TTGTTAACCCCCCCCCCCTCCCGGGCCTCCCCAACCCCCCCCAACCCGGTTGCCACCCCC
  CCCCCCGGTTACACGCCCCTCTCCACCCACCTAGTTCCCCCACCCCGTCTTTCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033554
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033554.2, NP_291032.2
• RefSeq Size: 1157 bp
• RefSeq ORF: 783 bp
• Locus ID: 3113
• UniProt ID: P20036
• Cytogenetics: 6p21.32
• Domains: MHC_II_alpha, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein.