EPX (NM_000502) Human Untagged Clone

CAT#: SC119861

EPX (untagged)-Human eosinophil peroxidase (EPX)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPX
• Synonyms: EPO; EPP; EPX-PEN; EPXD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119861 sequence for NM_000502 edited (data generated by NextGen Sequencing)
  ATGCATCTGCTCCCAGCCCTGGCAGGGGTCCTGGCCACACTCGTCCTCGCCCAGCCCTGT
  GAGGGCACTGACCCAGCCTCCCCTGGGGCAGTGGAGACCTCGGTCCTGCGAGACTGCATG
  GCAGAGGCCAAGTTGCTGGTGGATGCTGCCTACAATTGGACCCAGAAGAGCATCAAGCAG
  CGGCTTCGCAGCGGTTCAGCCAGCCCCATGGACCTCCTGTCCTACTTCAAACAACCGGTA
  GCAGCCACCAGGACAGTTGTTCGGGCCGCAGATTATATGCATGTGGCTTTGGGGCTGCTT
  GAAGAGAAGTTACAACCCCAGCGGTCCGGACCCTTCAATGTCACTGATGTGCTAACAGAA
  CCACACCTGCGGCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCGGGACCAGGCCGAGCGC
  TGCAGCGACAAGTACCGCACCATCACTGGACGGTGCAACAACAAGAGGAGACCCTTGCTA
  GGGGCCTCCAACCAGGCTCTGGCTCGCTGGCTGCCCGCCGAGTATGAGGATGGGCTGTCG
  CTCCCCTTCGGCTGGACCCCCAGCAGGAGGCGCAATGGCTTCCTTCTCCCTCTTGTCCGG
  GCTGTCTCCAACCAGATTGTGCGCTTCCCCAATGAGAGACTGACCTCCGACCGTGGCCGG
  GCCCTCATGTTCATGCAGTGGGGCCAGTTCATTGACCATGACCTGGACTTCTCCCCGGAG
  TCCCCGGCCAGAGTGGCCTTCACTGCAGGCGTTGACTGTGAGAGGACCTGCGCCCAGCTG
  CCCCCCTGCTTTCCCATCAAGATCCCACCCAATGACCCCCGCATCAAGAACCAGCGTGAC
  TGCATCCCTTTCTTCCGCTCGGCACCCTCATGCCCCCAAAACAAGAACAGAGTCCGCAAC
  CAGATCAACGCGCTCACCTCCTTTGTGGACGCCAGCATGGTGTATGGCAGTGAGGTCTCC
  CTCTCGCTGCGGCTCCGCAACCGGACCAACTACCTGGGGCTGCTGGCCATCAACCAGCGC
  TTTCAAGACAACGGCCGGGCCCTGCTGCCCTTCGACAACCTGCACGATGACCCCTGTCTC
  CTCACCAACCGCTCGGCGCGCATCCCCTGCTTCCTGGCAGGTGACACCCGATCAACGGAA
  ACCCCCAAACTGGCAGCCATGCACACCCTCTTTATGCGAGAGCACAACCGGCTGGCCACC
  GAGCTGAGACGCCTGAATCCCCGGTGGAATGGAGACAAACTGTACAATGAGGCTCGGAAG
  ATCATGGGGGCCATGGTCCAGATCATCACCTACCGAGACTTTCTGCCCCTGGTTCTGGGC
  AAGGCCCGGGCCAGGAGAACCCTGGGGCACTACAGGGGGTACTGCTCCAATGTGGACCCA
  CGGGTGGCCAATGTCTTCACCCTGGCCTTCCGCTTTGGCCACACAATGCTCCAGCCCTTC
  ATGTTCCGCTTGGACAGTCAGTACCGGGCCTCCGCACCCAACTCGCATGTCCCACTTAGC
  TCTGCCTTCTTTGCCAGCTGGCGGATCGTGTATGAAGGGGGCATCGACCCCATCCTCCGG
  GGCCTCATGGCCACCCCTGCCAAGCTGAACCGTCAGGATGCCATGTTAGTGGATGAGCTC
  CGGGACCGGCTGTTTCGGCAAGTGAGGAGGATTGGGCTGGACCTGGCAGCTCTCAACATG
  CAACGAAGCCGGGACCACGGCCTTCCAGGGTACAATGCTTGGAGGCGCTTCTGTGGGCTC
  TCCCAGCCCCGGAATTTGGCACAGCTTAGCCGGGTGCTGAAAAACCAGGACTTGGCAAGG
  AAGTTCCTGAATTTGTATGGAACACCTGACAACATTGACATCTGGATTGGGGCCATCGCT
  GAGCCTCTTTTGCCGGGGGCTCGAGTGGGGCCTCTTCTGGCTTGTCTGTTCGAGAACCAG
  TTCAGAAGAGCCCGAGACGGAGACAGGTTCTGGTGGCAGAAACGAGGTGTTTTCACCAAA
  AGACAGCGCAAGGCCCTGAGCAGAATTTCCTTGTCTCGAATTATATGTGACAATACCGGT
  ATCACCACGGTTTCAAGGGACATCTTCAGAGCCAACATCTACCCTCGGGGCTTTGTGAAC
  TGCAGCCGTATCCCCAGGTTGAACCTATCAGCCTGGCGAGGGACATGA
  
  Clone variation with respect to NM_000502.4
  120 a=>g;366 g=>c;660 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000502 unedited
  NCGTTAGGATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGAAGTGAG
  AGGTCGGCTGGGGGCCCTCAAGTGAGAGGGGAGCAGAGGATCCTCCCGTGCAGGCTGTGG
  ATGTCACTCACTTCCCAGCTGGTGAAGCCTCGCTGCAGAGATGCATCTGCTCCCAGCCCT
  GGCAGGGGTCCTGGCCACACTCGTCCTCGCCCAGCCCTGTGAGGGCACTGACCCAGCCTC
  CCCTGGGGCAGTGGAGACCTCGGTCCTGCGAGACTGCATGGCAGAGGCCAAGTTGCTGGT
  GGATGCTGCCTACAATTGGACCCAGAAGAGCATCAAGCAGCGGCTTCGCAGCGGTTCAGC
  CAGCCCCATGGACCTCCTGTCCTACTTCAAACAACCGGTAGCAGCCACCAGGACAGTTGT
  TCGGGCCGCAGATTATATGCATGTGGCTTTGGGGCTGCTTGAAGAGAAGTTACAACCCCA
  GCGGTCCGGACCCTTCAATGTCACTGATGTGCTAACAGAACCACACCTGCGGCTGCTGTC
  CCAGGCCAGTGGCTGTGCTCTCCGGGACCAGGCCGAGCGCTGCAGCGACAAGTACCGCAC
  CATCACTGGACGGTGCAACAACAAGAGGAGACCCTTGCTAGGGGCCTCCAACCAGGCTCT
  GGCTCGCTGGCTGCCCGCCGAGTATGAGGATGGGCTGTCGCTCCCCTTCGGCTGGACCCC
  CAGCAGGAGGCGCAATGGCTTCCTTCTCCCTCTTGTGCGGGCTGTCTCCACCAGATTGTG
  CGCTTCCCCAAGAGAGACTGACCTCCGACCGTGGCCGGGCCCTCATGTTCATGCAGTGGN
  GCCAGTTCATTGACCATGACCTGNNACTTCTCCCGNATCCCCGGNCAGAGTGCCCTCACT
  GCCAGCGTTGACTGTGAGAGGACTGCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000502 unedited
  GGGGGGGGGCGGGCCNAACCNNNNCCCTTTTNNNNNNGGNNTTTTACTTGNCCNCGGCCG
  CTTTTNANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCT
  TAACAGGGCTTTATTGGTAAAAACAAAACATGCTGCCTGGCCTCAAGCTCAAAAGAATAT
  TGTGGGGGGTCTGGAGGCTAAGACCCAATGGTCTCTTAATCATGGGGAGGGAGGGGAGGG
  AGGGCTGGGAAACAATAAAAAGGGCTGTAAACCCTCCAACTGACTTTTTTTGCCTGGGGG
  GCAAAACCACATGGCTTGGCTCCCAAACCTTTGCACGTGGCGCTGATTCATAACCTAATA
  CCTAAAAGGCTCCCAAGGCGGGAAGGGCCATTGCTGATAAGAACCCCCAACCTTCACTCC
  TGGCTGGGGAACCCAACTCTGAACTGTAACCAACAAAAACGTCAACAGCCTGCACTTGCT
  CCAAGGAAACAAGGCAACCTCATGGGCCTCCCCTTCCCCTTGTCTCCAAAAGTTGGAAAC
  TTGGGATAAACTCCTGCAAAAACCTCATGTCCCTCGCCAGGCTGATAGGGTCAACCTGGG
  GATACCGCTGCAGTTCACAAAGCCCCCAGGGTAGATGTTGGCTCTTAAGAAGTCCCTTGA
  AACCCTGGTGATACCGGTATTGTCACATATAATTCGAGACAAAGAAATTCTGCTCAAGGC
  CTTGCGCTGTCTTTTGGTGAAAACACCTCGTTTCTGCCACCAGAACCTGTCTTCCTCTCG
  GGCTCTCCTGAACTGGTTCTCGAACAGAAAGCCAGAAAAGCCCCACTCCACCCCCCGCAA
  AAAGCTCAACGATGGCCCCATCCAGATGTCATGTTGTCAGGGTTCCATACAAATTAGAAC
  CTCCTGGCCAACCTGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000502
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000502.2, NP_000493.1
• RefSeq Size: 2267 bp
• RefSeq ORF: 2148 bp
• Locus ID: 8288
• UniProt ID: P11678
• Cytogenetics: 17q22
• Domains: An_peroxidase
• Protein Families: Druggable Genome
• Protein Pathways: Asthma
• Gene Summary: This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016]