ALDH2 (NM_000690) Human Untagged Clone

CAT#: SC119703

ALDH2 (untagged)-Human aldehyde dehydrogenase 2 family (mitochondrial) (ALDH2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH2
• Synonyms: ALDH-E2; ALDHI; ALDM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119703 sequence for NM_000690 edited (data generated by NextGen Sequencing)
  ATGTTGCGCGCTGCCGCCCGCTTCGGGCCCCGCCTGGGCCGCCGCCTCTTGTCAGCCGCC
  GCCACCCAGGCCGTGCCTGCCCCCAACCAGCAGCCCGAGGTCTTCTGCAACCAGATTTTC
  ATAAACAATGAATGGCACGATGCCGTCAGCAGGAAAACATTCCCCACCGTCAATCCGTCC
  ACTGGAGAGGTCATCTGTCAGGTAGCTGAAGGGGACAAGGAAGATGTGGACAAGGCAGTG
  AAGGCCGCCCGGGCCGCCTTCCAGCTGGGCTCACCTTGGCGCCGCATGGACGCATCACAC
  AGGGGCCGGCTGCTGAACCGCCTGGCCGATCTGATCGAGCGGGACCGGACCTACCTGGCG
  GCCTTGGAGACCCTGGACAATGGCAAGCCCTATGTCATCTCCTACCTGGTGGATTTGGAC
  ATGGTCCTCAAATGTCTCCGGTATTATGCCGGCTGGGCTGATAAGTACCACGGGAAAACC
  ATCCCCATTGACGGAGACTTCTTCAGCTACACACGCCATGAACCTGTGGGGGTGTGCGGG
  CAGATCATTCCGTGGAATTTCCCGCTCCTGATGCAAGCATGGAAGCTGGGCCCAGCCTTG
  GCAACTGGAAACGTGGTTGTGATGAAGGTAGCTGAGCAGACACCCCTCACCGCCCTCTAT
  GTGGCCAACCTGATCAAGGAGGCTGGCTTTCCCCCTGGTGTGGTCAACATTGTGCCTGGA
  TTTGGCCCCACGGCTGGGGCCGCCATTGCCTCCCATGAGGATGTGGACAAAGTGGCATTC
  ACAGGCTCCACTGAGATTGGCCGCGTAATCCAGGTTGCTGCTGGGAGCAGCAACCTCAAG
  AGAGTGACCTTGGAGCTGGGGGGGAAGAGCCCCAACATCATCATGTCAGATGCCGATATG
  GATTGGGCCGTGGAACAGGCCCACTTCGCCCTGTTCTTCAACCAGGGCCAGTGCTGCTGT
  GCCGGCTCCCGGACCTTCGTGCAGGAGGACATCTATGATGAGTTTGTGGAGCGGAGCGTT
  GCCCGGGCCAAGTCTCGGGTGGTCGGGAACCCCTTTGATAGCAAGACCGAGCAGGGGCCG
  CAGGTGGATGAAACTCAGTTTAAGAAGATCCTCGGCTACATCAACACGGGGAAGCAAGAG
  GGGGCGAAGCTGCTGTGTGGTGGGGGCATTGCTGCTGACCGTGGTTACTTCATCCAGCCC
  ACTGTGTTTGGAGATGTGCAGGATGGCATGACCATCGCCAAGGAGGAGATCTTCGGGCCA
  GTGATGCAGATCCTGAAGTTCAAGACCATAGAGGAGGTTGTTGGGAGAGCCAACAATTCC
  ACGTACGGGCTGGCCGCAGCTGTCTTCACAAAGGATTTGGACAAGGCCAATTACCTGTCC
  CAGGCCCTCCAGGCGGGCACTGTGTGGGTCAACTGCTATGATGTGTTTGGAGCCCAGTCA
  CCCTTTGGTGGCTACAAGATGTCGGGGAGTGGCCGGGAGTTGGGCGAGTACGGGCTGCAG
  GCATACACTGAAGTGAAAACTGTCACAGTCAAAGTGCCTCAGAAGAACTCATAA
  
  Clone variation with respect to NM_000690.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000690 unedited
  GGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCCGCTGCGAT
  GTTGCGCGCTGCCGCCCGCTTCGGGCCCCGCCTGGGCCGCCGCCTCTTGTCAGCCGCCGC
  CACCCAGGCCGTGCCTGCCCCCAACCAGCAGCCCGAGGTCTTCTGCAACCAGATTTTCAT
  AAACAATGAATGGCACGATGCCGTCAGCAGGAAAACATTCCCCACCGTCAATCCGTCCAC
  TGGAGAGGTCATCTGTCAGGTAGCTGAAGGGGACAAGGAAGATGTGGACAAGGCAGTGAA
  GGCCGCCCGGGCCGCCTTCCAGCTGGGCTCACCTTGGCGCCGCATGGACGCATCACACAG
  GGGCCGGCTGCTGAACCGCCTGGCCGATCTGATCGAGCGGGACCGGACCTACCTGGCGGC
  CTTGGAGACCCTGGACAATGGCAAGCCCTATGTCATCTCCTACCTGGTGGATTTGGACAT
  GGTCCTCAAATGTCTCCGGTATTATGCCGGCTGGGCTGATAAGTACCACGGGAAAACCAT
  CCCCATTGACGGAGACTTCTTCAGCTACACACGCCATGAACCTGTGGGGGTGTGCGGGCA
  GATCATTCCGTGGAATTTCCCGCTCCTGATGCAAGCATGGAAGCTGGGCCCAGCCTTGGC
  AACTGGAAACGTGGTTGTGATGAAGGTAGCTGAGCAAACACCCCTCACCGCCCTCTATGT
  GGCCAACCTGATCAGGGAGCTGGCTTTCCCCCTGGTGTGTCAACATTGTGCCTGGATTTG
  CCCCCACGGTGGGGCCGCCATTGCCTCCATGAGGATGTGGACAAGTGGCATCACCAGCTC
  ACTGAGATGGCCGGTAACCACAGGTGCTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000690 unedited
  CGCAATCTATAGTCGAGTNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTAAAAACAGCTAGTTTTTAATGAATCTTACCCCAAAATACAAAGCAGGGGGGAAC
  AGTTGCCTTAATGGGGATATAATTGGAAACAATAGCTTTTTTTTCCCCTATAAACTCGAG
  TTATAGGAAGCGTTTCAAAAAGAGGATAACACATTTGAATCTATTTTTTAAAAATCATCC
  TGAAAGCTAACAGTATTGGTGGCGTTTAAAAGGGTCCTCATATACTCTTACCCTCAGCCA
  ACCCACCATGTTTATCAATTAAAATTCTAAAACTTTCTTGACCCAAAAAATTTTGGAGGA
  AAAATTTCTTTTTTCAAATTTTCAGCACGCAAGGATCATTTTTCTTGGTTTTGGGGCTGA
  TCTTGCTGAACTTTCCATCAATGGCTGAGGGAGGAAACCTGCACGATCCTTATGAGTCCT
  TTTGAGGCACTTTGACTGGGACAGTTTTCATTTCAGGGATTGCCTGGAGACCGTTCTCCG
  CCAACTCCGGAGCTTTCCCGATTTTTTGTTCCTTGAAGGGTGTTTGGGTTCTGTGTGTTC
  TGTGTGGGCCCCTTGTGTGTCGTCGTGGTGGCGTGGTTTGGCCTGGGTGGTGGCACGGGC
  TGGTGGGTGGGTGGGGGCGGCGTCTGCAGGCTGGAGGACTCCACTGCCTGCCCCAGGCCT
  GCGTGTTGCGGGCACTACTTGGCCCCCGTCGCCCCCTGTTTCTGCGTGTTTTCTCCTTGT
  CCCTCTCCCCCCTCCTCCTGTGCTCTTTTGCCCGCCTCGTCTTTTTCTCCTCACGCTTTC
  TCCTACCACTCTTTGGCGTGAACATACTTTCTCCTATCTCTCACCCCGCTCCGGTCCCCG
  CCCCCCCTCGCGGCCGCTCCGCCTTCTTACTCCCCGCCCCCTCCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000690
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000690.2, NP_000681.2
• RefSeq Size: 2445 bp
• RefSeq ORF: 1554 bp
• Locus ID: 217
• UniProt ID: P05091
• Cytogenetics: 12q24.12
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).