Cytochrome P450 1A2 (CYP1A2) (NM_000761) Human Untagged Clone

CAT#: SC119671

CYP1A2 (untagged)-Human cytochrome P450, family 1, subfamily A, polypeptide 2 (CYP1A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 1A2
• Synonyms: CP12; CYPIA2; P3-450; P450(PA)
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000761 edited
  GCACCAGGCCATTACAACCCTGCCAATCTCAAGCACCTGCCTCTACAGTTGGTACAGATG
  GCATTGTCCCAGTCTGTTCCCTTCTCGGCCACAGAGCTTCTCCTGGCCTCTGCCATCTTC
  TGCCTGGTATTCTGGGTGCTCAAGGGTTTGAGGCCTCGGGTCCCCAAAGGCCTGAAAAGT
  CCACCAGAGCCATGGGGCTGGCCCTTGCTCGGGCATGTGCTGACCCTGGGGAAGAACCCG
  CACCTGGCACTGTCAAGGATGAGCCAGCGCTACGGGGACGTCCTGCAGATCCGCATTGGC
  TCCACGCCCGTGCTGGTGCTGAGCCGCCTGGACACCATCCGGCAGGCCCTGGTGCGGCAG
  GGCGACGATTTCAAGGGCCGGCCTGACCTCTACACCTCCACCCTCATCACTGATGGCCAG
  AGCTTGACCTTCAGCACAGACTCTGGACCGGTGTGGGCTGCCCGCCGGCGCCTGGCCCAG
  AATGCCCTCAACACCTTCTCCATCGCCTCTGACCCAGCTTCCTCATCCTCCTGCTACCTG
  GAGGAGCATGTGAGCAAGGAGGCTAAGGCCCTGATCAGCAGGTTGCAGGAGCTGATGGCA
  GGGCCTGGGCACTTCGACCCTTACAATCAGGTGGTGGTGTCAGTGGCCAACGTCATTGGT
  GCCATGTGCTTCGGACAGCACTTCCCTGAGAGTAGCGATGAGATGCTCAGCCTCGTGAAG
  AACACTCATGAGTTCGTGGAGACTGCCTCCTCCGGGAACCCCCTGGACTTCTTCCCCATC
  CTTCGCTACCTGCCTAACCCTGCCCTGCAGAGGTTCAAGGCCTTCAACCAGAGGTTCCTG
  TGGTTCCTGCAGAAAACAGTCCAGGAGCACTATCAGGACTTTGACAAGAACAGTGTCCGG
  GACATCACGGGTGCCCTGTTCAAGCACAGCAAGAAGGGGCCTAGAGCCAGCGGCAACCTC
  ATCCCACAGGAGAAGATTGTCAACCTTGTCAATGACATCTTTGGAGCAGGATTTGACACA
  GTCACCACAGCCATCTCCTGGAGCCTCATGTACCTTGTGACCAAGCCTGAGATACAGAGG
  AAGATCCAGAAGGAGCTGGACACTGTGATTGGCAGGGAGCGGCGGCCCCGGCTCTCTGAC
  AGACCCCAGCTGCCCTACTTGGAGGCCTTCATCCTGGAGACCTTCCGACACTCCTCCTTC
  TTGCCCTTCACCATCCCCCACAGCACAACAAGGGACACAACGCTGAATGGCTTCTACATC
  CCCAAGAAATGCTGTGTCTTCGTAAACCAGTGGCAGGTCAACCATGACCCAGAGCTGTGG
  GAGGACCCCTCTGAGTTCCGGCCTGAGCGGTTCCTCACCGCCGATGGCACTGCCATTAAC
  AAGCCCTTGAGTGAGAAGATGATGCTGTTTGGCATGGGCAAGCGCCGGTGTATCGGGGAA
  GTCCTGGCCAAGTGGGAGATCTTCCTCTTCCTGGCCATCCTGCTACAGCAACTGGAGTTC
  AGCGTGCCGCCGGGCGTGAAAGTCGACCTGACCCCCATCTACGGGCTGACCATGAAGCAC
  GCCCGCTGTGAACATGTCCAGGCGCGGCTGCGCTTCTCCATCAACTGAAGAAGACACCAC
  CATTCTGAGGCCAGGGAGCGAGTGGGGGCCAGCCACGGGGACTCAGCCCTTGTTTCTCTT
  CCTTTCTTTTTTTAAAAAATAGCAGCTTTAGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000761 unedited
  NGTTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACCAGATTACAACCC
  TGCCAATCTCAAGCACCTGCCTCTACAGTTGGTACAGATGGCATTGTCCCAGTCTGTTCC
  CTTCTCGGCCACAGAGCTTCTCCTGGCCTCTGCCATCTTCTGCCTGGTATTCTGGGTGCT
  CAAGGGTTTGAGGCCTCGGGTCCCCAAAGGCCTGAAAAGTCCACCAGAGCCATGGGGCTG
  GCCCTTGCTCGGGCATGTGCTGACCCTGGGGAAGAACCCGCACCTGGCACTGTCAAGGAT
  GAGCCAGCGCTACGGGGACGTCCTGCAGATCCGCATTGGCTCCACGCCCGTGCTGGTGCT
  GAGCCGCCTGGACACCATCCGGCAGGCCCTGGTGCGGCAGGGCGACGATTTCAAGGGCCG
  GCCTGACCTCTACACCTCCACCCTCATCACTGATGGCCAGAGCTTGACCTTCAGCACAGA
  CTCTGGACCGGTGTGGGCTGCCCGCCGGCGCCTGGCCCAGAATGCCCTCAACACCTTCTC
  CATCGCCTCTGACCCAGCTTCCTCATCCTCCTGCTACCTGGAGGAGCATGTGAGCAAGGA
  GGCTAAGGCCCTGATCAGCAGGTTGCAGGAGCTGATGGCAGGGCCTGGGCACTTCGACCC
  TTACAATCAGGTGGTGGTGTCAGTGGCCAACGTCATTGGTGCCATGTGCTTCGGACAGCA
  CTTCCCTGAGAGTAGCGATGAGATGCTCAGCCTCGTGAAGACACTCATGAGTTCGTGGAG
  ACTGCCTCCTNCGGGAACCCCCTGGACTTCTTCCCCATCCTTCGCTACCTGCCTAACCCT
  GCCCTGCAGAGGTTCAAGGCCTTTCACCAGAGTTCCCTGTGGTTCCTGCAAANACAGTCC
  AGGAGCACTATCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000761 unedited
  TATCTTGNCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTCATTGGGTA
  ATTCCATTTATATGAAATGTTTAGAGAAGGCAAATCCATAGACACAGAAAGTAGATTAGC
  AGTTGCCAGGGCTGGTGAGGCAGGGGAGGCAGGCTAGGGGGAAATGTGGAGGGACTGCTA
  ATGGGTGCAGGGTTTCTTTTAGGGGTGATGAAAATGTTCTAAAATTAATTGTGGGCCGGG
  CACCGTGGCTCACACCTGTAATGCCAGCACTTTGAGAGAGGTCGAGGCGGGCAGATCACT
  TGAGGTCAAAAGTTTGAGACTAGTCTAGCCAACATGGTGAAACCCGTCTCTACTAAAAAT
  ACAAAAATTACCCAGGCGTGGTGGTGTGTGCCTGTAGTCTCAGCTACTTGGGAGGCTGAG
  GCTGGAGAAGCACTTGAACCTGGGAGGCAAAGGTTGCAGTGAGCTGAGATGATGCCACTG
  CACTCCAGCTTAGGAGACAGAGGAAGACTCTGTCTCAAAAAAAAAAAAAAACAAAAAGGC
  TGGGCGCGGTGGTTCATACCTGTTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATC
  ACGAGGTCAAGAGATGGAGACCATCCTGNCCAACACAGTGAAACCTCGTCTCTACTAAAA
  AACAAAAATAGCTGGCCATGGTGGCGCATGCCTATAGTCCCAGCTACTTCGGAGGGGCTG
  AGCAGAGAATCCCTGAACATGNNGAGCGGAGTTGTANTGACCTGAAATCGCGCACTGCAC
  TCAGCCTGGCAACAGAGTAGACTCTGTCTCAAANNTATAGAAATATAAATTAATTCTGGG
  ATGGGTGCACAATNCACACTAAAAATCATTCGACCTTAAAATGTAGTTAGGCTGGATGGG
  NNGGCTACACCTGNATCCAGNACTTTGGAACCAAGCCAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000761
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000761.3, NP_000752.2
• RefSeq Size: 3127 bp
• RefSeq ORF: 1551 bp
• Locus ID: 1544
• UniProt ID: P05177
• Cytogenetics: 15q24.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Caffeine metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tryptophan metabolism
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]