HSD3B1 (NM_000862) Human Untagged Clone

CAT#: SC119611

HSD3B1 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD3B1
• Synonyms: 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119611 sequence for NM_000862 edited (data generated by NextGen Sequencing)
  ATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTTTCTGGGACAGAGGATCATC
  CGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGTCTTGGACAAGGCCTTCGGA
  CCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGACCAAGCTGACAGTGCTGGAA
  GGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATC
  CACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAGAGAGTCTATCATGAATGTC
  AATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCAAGCTAGTGTGCCAGTCTTC
  ATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTACAAGGAAATCATCCAGAAT
  GGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCCATACCCACACAGCAAAAAG
  CTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCTGAAAAACGGCGGCACCCTG
  TACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGGAAGCCGATTCCTTTCTGCT
  AGTATAAACGAGGCCCTGAACAACAATGGGATCCTGTCAAGTGTTGGAAAGTTCTCCACT
  GTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATTCTGGCCTTGAGGGCCCTG
  CAGGACCCCAAGAAGGCCCCAAGCATCCGAGGACAGTTCTACTATATCTCAGATGACACG
  CCTCACCAAAGCTATGATAACCTTAATTACACCCTGAGCAAAGAGTTCGGCCTCCGCCTT
  GATTCCAGATGGAGCTTTCCTTTATCCCTGATGTATTGGATTGGCTTCCTGCTGGAAATA
  GTGAGCTTCCTACTCAGGCCAATTTACACCTATCGACCGCCCTTCAACCGCCACATAGTC
  ACATTGTCAAATAGCGTATTCACCTTCTCTTATAAGAAGGCTCAGCGAGATTTGGCGTAT
  AAGCCACTCTACAGCTGGGAGGAAGCCAAGCAGAAAACGGTGGAGTGGGTTGGTTCCCTT
  GTGGACCGGCACAAGGAGAACCTGAAGTCCAAGACTCAGTGA
  
  Clone variation with respect to NM_000862.2
  1012 c=>t;1100 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000862 unedited
  GGGCGGTCATAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCCCTC
  TCCGGGTCACCCTAGNATCAGATCTGCTCCCCAGCATCTTCTGTTTCCTGGTGAGTGATT
  CCTGCTACTTTGGATGGCCATGACGGGCTGGAGCTGCCTTGTGACAGGAGCAGGAGGGTT
  TCTGGGACAGAGGATCATCCGCCTCTTGGTGAAGGAGAAGGAGCTGAAGGAGATCAGGGT
  CTTGGACAAGGCCTTCGGACCAGAATTGAGAGAGGAATTTTCTAAACTCCAGAACAAGAC
  CAAGCTGACAGTGCTGGAAGGAGACATTCTGGATGAGCCATTCCTGAAGAGAGCCTGCCA
  GGACGTCTCGGTCATCATCCACACCGCCTGTATCATTGATGTCTTCGGTGTCACTCACAG
  AGAGTCTATCATGAATGTCAATGTGAAAGGTACCCAGCTCCTGTTAGAGGCCTGTGTCCA
  AGCTAGTGTGCCAGTCTTCATCTACACCAGTAGCATAGAGGTAGCCGGGCCCAACTCCTA
  CAAGGAAATCATCCAGAATGGCCATGAAGAAGAGCCTCTGGAAAACACATGGCCCGCTCC
  ATACCCACACAGCAAAAAGCTTGCTGAGAAGGCTGTACTGGCGGCTAACGGGTGGAATCT
  GAAAAACGGCGGCACCCTGTACACTTGTGCCTTACGACCCATGTATATCTATGGGGAAGG
  AAGCCGATTCCTTTCTGCTAGTATAAACGAGGCCCTGAACACAATGGGATCCTGTCAAGT
  GTNGGAAAAGTCTCCACTGTTAACCCAGTCTATGTTGGCAATGTGGCCTGGGCCCACATT
  CTGGCCTTGAGGNCCCCCTGCAGACCCCAGAAGGGCCCAGCATNNCGAGACAGTTCTACT
  ATATCTCAGATGACACGCCTCACCAAAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000862
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000862.1, NP_000853.1
• RefSeq Size: 1665 bp
• RefSeq ORF: 1122 bp
• Locus ID: 3283
• UniProt ID: P14060
• Cytogenetics: 1p12
• Domains: 3Beta_HSD
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]