BMP6 (NM_001718) Human Untagged Clone

CAT#: SC119057

BMP6 (untagged)-Human bone morphogenetic protein 6 (BMP6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BMP6
• Synonyms: VGR; VGR1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001718 edited
  ATGCCGGGGCTGGGGCGGAGGGCGCAGTGGCTGTGCTGGTGGTGGGGGCTGCTGTGCAGC
  TGCTGCGGGCCCCCGCCGCTGCGGCCGCCCTTGCCCGCTGCCGCGGCCGCCGCCGCCGGG
  GGGCAGCTGCTGGGGGACGGCGGGAGCCCCGGCCGCACGGAGCAGCCGCCGCCGTCGCCG
  CAGTCCTCCTCGGGCTTCCTGTACCGGCGGCTCAAGACGCAGGAGAAGCGGGAGATGCAG
  AAGGAGATCTTGTCGGTGCTGGGGCTCCCGCACCGGCCCCGGCCCCTGCACGGCCTCCAA
  CAGCCGCAGCCCCCGGCGCTCCGGCAGCAGGAGGAGCAGCAGCAGCAGCAGCAGCTGCCT
  CGCGGAGAGCCCCCTCCCGGGCGACTGAAGTCCGCGCCCCTCTTCATGCTGGATCTGTAC
  AACGCCCTGTCCGCCGACAACGACGAGGACGGGGCGTCGGAGGGGGAGAGGCAGCAGTCC
  TGGCCCCACGAAGCAGCCAGCTCGTCCCAGCGTCGGCAGCCGCCCCCGGGCGCCGCGCAC
  CCGCTCAACCGCAAGAGCCTTCTGGCCCCCGGATCTGGCAGCGGCGGCGCGTCCCCACTG
  ACCAGCGCGCAGGACAGCGCCTTCCTCAACGACGCGGACATGGTCATGAGCTTTGTGAAC
  CTGGTGGAGTACGACAAGGAGTTCTCCCCTCGTCAGCGACACCACAAAGAGTTCAAGTTC
  AACTTATCCCAGATTCCTGAGGGTGAGGTGGTGACGGCTGCAGAATTCCGCATCTACAAG
  GACTGTGTTATGGGGAGTTTTAAAAACCAAACTTTTCTTATCAGCATTTATCAAGTCTTA
  CAGGAGCATCAGCACAGAGACTCTGACCTGTTTTTGTTGGACACCCGTGTAGTATGGGCC
  TCAGAAGAAGGCTGGCTGGAATTTGACATCACGGCCACTAGCAATCTGTGGGTTGTGACT
  CCACAGCATAACATGGGGCTTCAGCTGAGCGTGGTGACAAGGGATGGAGTCCACGTCCAC
  CCCCGAGCCGCAGGCCTGGTGGGCAGAGACGGCCCTTACGACAAGCAGCCCTTCATGGTG
  GCTTTCTTCAAAGTGAGTGAGGTGCACGTGCGCACCACCAGGTCAGCCTCCAGCCGGCGC
  CGACAACAGAGTCGTAATCGCTCTACCCAGTCCCAGGACGTGGCGCGGGTCTCCAGTGCT
  TCAGATTACAACAGCAGTGAATTGAAAACAGCCTGCAGGAAGCATGAGCTGTATGTGAGT
  TTCCAAGACCTGGGATGGCAGGACTGGATCATTGCACCCAAGGGCTATGCTGCCAATTAC
  TGTGATGGAGAATGCTCCTTCCCACTCAACGCACACATGAATGCAACCAACCACGCGATT
  GTGCAGACCTTGGTTCACCTTATGAACCCCGAGTATGTCCCCAAACCGTGCTGTGCGCCA
  ACTAAGCTAAATGCCATCTCGGTTCTTTACTTTGATGACAACTCCAATGTCATTCTGAAA
  AAATACAGGAATATGGTTGTAAGAGCTTGTGGATGCCACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001718 unedited
  TTACGCGCCCGTTGCCGCTTATGGGCGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGGGGCAGCGCACGTGTGCGGCCAGCGAGGCCCAGAGTGACCGCGCCGCG
  ACTCGCAGGAGCCAGGGCGCATGCTGCAGTGCAGCCCGGACTCAGACGCACCTGGCCTGG
  ACCGCGCGCCTCTAGAGACCTGCGCGAGGCTGTGAGGCTCCCCTTCCTCCCCTCCAAGCG
  GTTCTCCTGGTGATCGCCCCTTCGCCACCTCTCTAGCCTGGGCAACTGGGGGCGCCCCGG
  ACGACCATGAGAGATAAGGACTGAGGGCCAGGAAGGGGAAGCGAGCCCGCCGAGAGGTGG
  CGGGGACTGCTCACGCCAAGGGCCACAGCGGCCGCGCTCCGGCCTCGCTCCGCCGCTCCA
  CGCCTCGCGGGATCCGCGGGGGCAGCCCGGCCGGGCGGGGATGCCGGGGCTGGGGCGGAG
  GGCGCAGTGGCTGTGCTGGTGGTGGGGGCTGCTGTGCAGCTGCTGCGGGCCCCCGCCGCT
  GCGGCCGCCCTTGCCCGCTGCCGCGGCCGCCGCCGCCGGGGGGCAGCTGCTGGGGGACAG
  CGGGAGCCCCGGCCGCACGGAGCAGCCGCCGCCGTCGCCGCAGTCCTCCTCGGGCTTCCT
  GTACCGGCGGCTCAAGACGCATGAGAAGCGGGAGATGCAGAAGGAGATCTTGTCGGTGCT
  GGGGCTNCCGCACCGGCCCCGCCCCTGCACGGNCCTCCACAGCCGCAGCCCCCGGCGCTT
  CGGCAGCAAGAGGAGCATCAGCAGCAGCAGCAGCTGCCTGGCGGAAAGCCCCCTCCCGGG
  CGACTTGAATGCCGGGCCCCTCTCATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001718 unedited
  AAAAAAGCCTCGGGGTGGCACCCTCCTTGCCGGGAAAGCACTGGGCCAGGGTCACAGGGC
  TGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTT
  TTTTTTTTTTTTTTTTCGACCAGCCTCTGCCTTAAAGGAAATCTTTATTAATCACGTATG
  GTTCACAGATAATTCTTTTTTTAAAAAAACCCAACCTTCTATAGAAGCACAACTGTCAAG
  AGTCTTGTACACACAACTTCAGCTTTGCATCACGAGTCTTGTATTCCAAGAAAATCAAAG
  TGGTACAATTTGTTTGTTTACACTATGATACTTTCTAAATAAACTCTTTTTTTTTAAAAG
  TCTGGTCTTTCCTTCAATGTTACAGCAAAACAGATATAAAATAGACAATAAATTATAGTT
  TATATTTACAAAAAAAGCTGTAAGTGCAAACAGTTGTAGATTATAAATGTATTATTTAAT
  CAGTTTAGTATGAAATTGCCTTCCCAGTACATGATTGTGAAAAAGACATTTAGAAAATAT
  TCTAAAATTTAATCTGAGCCTCACTTTCTACAAGGGAAATCATGATTTCCGTTCATAAAC
  AGCATGCTCATCCCCCTAACACCATTCTTATAAGCTGGGCACCCTCATTTTATTTTCTTC
  GTTGGTTCTAACCCTGTGGCGTGGTATGCTGTATAGTAAAAAGGCAGAGAACCACTTTAC
  TGAAAAGGTACTAGAGCCGGCAGTCCAGAAGTTAATGTGCTGGTCAAAGAACCGTTCTGG
  GTAAGAAGAGGTGAGCATTGCCTTCACGTGTTACACGGTTACACACCCCTTGTAGCCTCN
  ACCTCAGTGTATCAGTCTACTTTTGGTACTAGCAAAAAGTACAGCAAATGGAGGATTGAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001718
• Insert Size: 1542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001718.5, NP_001709.1
• RefSeq Size: 3807 bp
• RefSeq ORF: 1542 bp
• Locus ID: 654
• UniProt ID: P22004
• Cytogenetics: 6p24.3
• Domains: TGFb_propeptide, TGF-beta
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
• Protein Pathways: Hedgehog signaling pathway, TGF-beta signaling pathway
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates a wide range of biological processes including iron homeostasis, fat and bone development, and ovulation. Differential expression of this gene may be associated with progression of breast and prostate cancer. Mutations in this gene may be associated with iron overload in human patients. [provided by RefSeq, Jul 2016]