EHHADH (NM_001966) Human Untagged Clone

CAT#: SC118906

EHHADH (untagged)-Human enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EHHADH
• Synonyms: ECHD; FRTS3; L-PBE; LBFP; LBP; MFE1; PBFE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001966 edited
  GCGAATTCGGCACGAGACATGGCCGAGTATACGCGGCTGCACAACGCCTTGGCGCTAATC
  CGCCTCCGAAACCCGCCGGTCAACGCGATCAGTACGACTTTACTCCGTGACATAAAAGAA
  GGACTACAGAAAGCTGTAATAGACCATACAATAAAAGCCATTGTGATTTGTGGAGCAGAG
  GGCAAATTTTCTGCAGGTGCTGATATTCGTGGCTTCAGTGCTCCTAGGACATTTGGCCTT
  ACACTGGGACATGTAGTAGATGAAATACAGAGAAATGAGAAGCCCGTGGTGGCAGCAATC
  CAAGGCATGGCTTTCGGAGGGGGACTAGAGCTGGCCCTGGGCTGTCACTATAGGATTGCC
  CACGCAGAGGCTCAAGTTGGCTTACCAGAAGTTACACTGGGACTTCTCCCTGGTGCAAGA
  GGAACCCAGCTTCTCCCCAGACTCACTGGAGTTCCTGCTGCACTTGACTTAATTACCTCA
  GGAAGACGTATTTTAGCAGATGAAGCACTCAAGCTGGGCATTCTAGATAAAGTTGTAAAC
  TCAGACCCGGTTGAAGAAGCAATCAGATTTGCTCAGAGAGTTTCAGATCAACCTCTAGAA
  TCCCGTAGACTCTGCAACAAGCCAATTCAGAGCTTGCCCAACATGGACAGCATTTTTAGT
  GAGGCCCTCTTGAAGATGCGGAGGCAGCACCCTGGGTGTCTTGCACAGGAGGCTTGTGTC
  CGTGCAGTCCAGGCTGCTGTGCAGTATCCCTATGAAGTGGGCATCAAGAAGGAGGAGGAG
  CTGTTTCTATATCTTTTGCAATCAGGGCAGGCTAGAGCCCTGCAATATGCTTTCTTCGCT
  GAAAGGAAAGCAAATAAGTGGTCAACTCCCTCCGGAGCATCGTGGAAAACAGCATCAGCG
  CGGCCTGTCTCCTCAGTTGGTGTTGTTGGCTTGGGAACAATGGGCCGAGGCATTGTCATT
  TCTTTTGCAAGGGCCAGGATTCCTGTGATTGCTGTAGACTCGGACAAAAACCAGCTAGCA
  ACTGCAAACAAGATGATAACCTCTGTCTTGGAAAAAGAAGCCTCCAAAATGCAACAGAGC
  GGCCACCCTTGGTCAGGACCAAAACCCAGGTTAACTTCATCTGTGAAGGAGCTTGGTGGT
  GTAGATTTAGTCATTGAAGCAGTATTTGAGGAAATGAGCCTGAAGAAGCAGGTCTTTGCT
  GAACTCTCAGCTGTGTGCAAACCAGAAGCATTTTTGTGCACTAATACTTCAGCCCTGGAT
  GTTGATGAGATTGCTTCTTCCACTGATCGTCCTCACTTGGTCATTGGCACCCACTTCTTT
  TCGCCAGCTCATGTCATGAAGTTGTTAGAGGTTATTCCCAGCCAATACTCTTCCCCCACT
  ACCATTGCCACTGTTATGAACTTATCAAAAAAGATTAAAAAGATTGGAGTCGTTGTAGGC
  AACTGTTTTGGATTTGTGGGGAATCGAATGTTGAATCCTTACTACAATCAGGCATATTTC
  TTGTTAGAAGAAGGCAGCAAACCAGAGGAGGTAGATCAGGTGCTGGAAGAGTTTGGTTTT
  AAAATGGGACCTTTTAGAGTGTCTGATCTTGCTGGGTTGGATGTGGGCTGGAAATCTAGA
  AAGGGGCAAGGTCTTACTGGACCTACATTGCTTCCAGGAACTCCTGCCCGAAAAAGGGGT
  AATAGGAGGTACTGCCCAATTCCTGATGTGCTCTGTGAATTAGGACGATTTGGCCAGAAG
  ACAGGTAAGGGTTGGTATCAATATGACAAGCCATTGGGTAGGATTCACAAACCTGATCCC
  TGGCTTTCCAAATTCCTATCACGGTATAGAAAAACCCATCACATTGAACCACGTACCATT
  AGCCAGGATGAGATCCTTGAACGCTGCTTATATTCACTTATCAATGAAGCATTCCGTATC
  TTGGGAGAAGGGATAGCTGCTAGCCCAGAGCACATTGATGTTGTCTATTTACATGGATAT
  GGATGGCCAAGGCACAAGGGCGGGCCCATGTTCTATGCTTCCACAGTTGGGTTGCCCACA
  GTTCTAGAGAAATTGCAGAAATATTACAGGCAGAACCCTGATATTCCCCAACTGGAGCCA
  AGTGACTATCTAAAAAAACTGGCTTCTCAGGGAAACCCTCCCCTGAAAGAATGGCAAAGC
  TTGGCAGGCTCCCCTAGCAGTAAATTGTGATTCAGTCTTCCAGATTATGCCTCACATGCT
  AGCATCAGGTAATGCTGACTGAATTTCAGTGAAATTAAATCAAAAATCCAAAGTAAGATT
  GTTCTGAAATACAAAGCAAAATAAATAATCATTAGAATCTTCTGTGTAACGACTCTAATG
  GTCAAATCTTTAGGAATGTGCTTCCTATGCCTCTGAATCTGTCCTTATCAGATAAATTCA
  ATGCATGAACTTGTGTGAATATAATACCATAATAGCTAATGAAAGAGGCTCAAGCATAAG
  TTGAGATTCTCAAATGCTTTTATCATTGGATAAATGTGTCATCAATTAATAAATGATAAA
  TGCAGCTAAGT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001966 unedited
  AGCTCGNGATTTNGTAAACGCACTTCATATAGGGCGGACAAGCCATTCGGCACGAGAACA
  TGGCCGAGTATACGCGGCTGCACAACGCCTTGGCGCTAATCCGCCTCCGAAACCCGCCGG
  TCAACGCGATCAGTACGACTTTACTCCGTGACATAAAAGAAGGACTACAGAAAGCTGTAA
  TAGACCATACAATAAAAGCCATTGTGATTTGTGGAGCATTTTTTAAATTTTCTGCAGGTG
  CTGATATTCGTGGCTTCAGTGCTCCTAGGACATTTGGCCTTACACTGGGACATGTAGTAG
  ATGAAATACAGAGAAATGAGAAGCCCGTGGTGGCAGCAATCCAAGGCATGGCTTTCGGAG
  GGGGACTAGAGCTGGCCCTGGGCTGTCACTATAGGATTGCCCACGCAGAGGCTCAAGTTG
  GCTTACCAGAAGTTACACTGGGACTTCTCCCTGGTGCAAGAGGAACCCAGCTTCTCCCCA
  GACTCACTGGAGTTCCTGCTGCACTTGACTTAATTACCTCAGGAAGACGTATTTTAGCAG
  ATGAAGCACTCAAGCTGGGCATTCTAGATAAAGTTGTAAACTCAGACCCGGTTGAAGAAG
  CAATCAGATTTGCTCAGAGAGTTTCAGATCAACCTCTAGAATCCCGTAGACTCTGCAACA
  AGCCAATTCAGAGCTTGCCCAACATGGACAGCATTTTTAGTGAGGCCCTCTTGAAGATGC
  GGAGGCAGCACCCTGNGTGTCTTGCACAGGAGGCTTGTGTCCGTGCAGTCCAGGCTGCTG
  TGCAGTATCCCTATGAAGTGGGCATNCAGAANGGAGAGGAGCTGTTTCTATATCTTTTGC
  AATCAGGGCAGGCTAGAGCCCTGCCATATGCTTTTCTTCGCTGAAAAGGAAAGCAAATAG
  TGGTCAACTTCCTCCGGAGCATCGTGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001966 unedited
  TCCCCNNNCCAAAATTTTNCCCTNNNGCCCTCCCCNCCCCGGACTCTAGCCCGNGGCCCG
  CCATNCTACATNCGGTTTTTTTTTTTTTTTTTTTTACTACTTTCCAGCAATCATTTTTAT
  TGATGTCTTTAATTTCACAAACCAAATTATATAAAATTAAGTAGTACACATTTCAAACAT
  AATAATTGTGCTCTGTACAATTCAAGGCACACTCGGATTCTTCGTTGGTTTTTTGCCCTG
  CTTTTTAACAACGGTAAAACTTATACTCCCATTCACAGATTCTTCCCATCACGAACTTTA
  ATCATTCCTATTCCGGCCTCACACGGTGAATACATTCGCGAAAGCTCATTTACACCACAA
  TGAAGGATACCATCTTCAGACTTATTTATTCACTAACAACTTTATTGACATATATTTTAC
  ATACCCTATCATTCACCCACTTAAAATACTTTCCTCTATGCCCTTTAACATATTGCCACG
  CGACTACCACCACACTCCCTTTATACCATTCCCCCGACCCATAAATAAATCTTGCCCCTA
  CAGATGAATTTCCCCCCCCCCCTTCATCCCTCCCAATTTTTCCCCCACCCCACCCCATGC
  CCCGTAAGTTTCCCTGACTTCACCGCCTTCCCCTTTGTTCCCCCTCCTCCACCCAATCCA
  CTGCCCTCACCTACTCTCCCTCCCCTCATACTTCCCCCCCCCCCCCCCCCCCTTCTCTCC
  ACCCCTTGTTTCTCCCCTTCTTCTCCCCCCCTCTCTCTTCCTCCTCCTCCGCTACCCCAA
  CTCTTCCACCCCCCCCCGTTTCCAAAAACCATTTTTCACCCCCTCACCACCTTCCGCCCC
  CCCTCCCTCTTAGTCCCTTAACACCTATTCTCCCCCTCCTCCACCCGCATTTAAATTATT
  ATTCGCCCCCACCCCCCCACATTTTTCATCTCCTTCACCCCACTTAACAACAATCTCACA
  CATTTCTCACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001966
• Insert Size: 3520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001966.2, NP_001957.2
• RefSeq Size: 3821 bp
• RefSeq ORF: 2172 bp
• Locus ID: 1962
• UniProt ID: Q08426
• Cytogenetics: 3q27.2
• Domains: ECH, 3HCDH, 3HCDH_N
• Protein Pathways: beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longer isoform (1).