Glutathione Peroxidase 4 (GPX4) (NM_002085) Human Untagged Clone

CAT#: SC118821

GPX4 (untagged)-Human glutathione peroxidase 4 (phospholipid hydroperoxidase) (GPX4), transcript variant 1 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: Glutathione Peroxidase 4
• Synonyms: GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002085, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTCGGCCGCCTTTGCCGCCTACTGAAGCCGGCGCTGCTCTGTGGGGCTCTGGCCGCGCCTGGCC
  TGGCCGGGACCATGTGCGCGTCCCGGGACGACTGGCGCTGTGCGCGCTCCATGCACGAGTTTTCCGCCAA
  GGACATCGACGGGCACATGGTTAACCTGGACAAGTACCGGGGCTTCGTGTGCATCGTCACCAACGTGGCC
  TCCCAGTGAGGCAAGACCGAAGTAAACTACACTCAGCTCGTCGACCTGCACGCCCGATACGCTGAGTGTG
  GTTTGCGGATCCTGGCCTTCCCGTGTAACCAGTTCGGGAAGCAGGAGCCAGGGAGTAACGAAGAGATCAA
  AGAGTTCGCCGCGGGCTACAACGTCAAATTCGATATGTTCAGCAAGATCTGCGTGAACGGGGACGACGCC
  CACCCGCTGTGGAAGTGGATGAAGATCCAACCCAAGGGCAAGGGCATCCTGGGAAATGCCATCAAGTGGA
  ACTTCACCAAGTTCCTCATCGACAAGAACGGCTGCGTGGTGAAGCGCTACGGACCCATGGAGGAGCCCCT
  GGTGATAGAGAAGGACCTGCCCCACTATTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002085 unedited
  CCCCCGATAGGATTTGTATACGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGTGGG
  GGAGCCAGGAGGGGCGGGCGACGGGCGGGTATGGGCCGCGCGGGCGCAGGCTCCCCCGGG
  CGCCGCAGGCAGCGGTGCCAGAGCCGGGGCAGGCGGCGGCCGCGAGCCCCTCGGCGGCGG
  AAGGCCCCAGCGTGCAGGCGCAGGAGGGCGCGGCGCCGGCGGAAGAAGCCCTGTCCCCGC
  AGCTTGCGACCGGAGATCCACGAATGTCCCAAGTCCCAGGACCCGTGCGCGTCCCGGGAC
  GACTGGCGCTGTGCGCGCTCCATGCACGAGTTTTCCGCCAAGGACATCGACGGGCACATG
  GTTAACCTGGACAAGTACCGGGGCTTCGTGTGCATCGTCACCAACGTGGCCTCCCAGTGA
  GGCAAGACCGAAGTAAACTACACTCAGCTCGTCGACCTGCACGCCCGATACGCTGAGTGT
  GGTTTGCGGATCCTGGCCTTCCCGTGTAACCAGTTCGGGAAGCAGGAGCCAGGGAGTAAC
  GAAGAGATCAAAGAGTTCGCCGCGGGCTACAACGTCAAATTCGATATGTTCAGCAAGATC
  TGCGTGAACGGGGACGACGCCCACCCGCTGTGGAAGTGGATGAAGATCCAACCCAAGGGC
  AAGGGCATCCTGTGAAATGCCATCAAGTGGAACTTCACCAAGTTCCTCATCGACAAGACG
  GCTGCGTGGTGAAGCGCTACTGACCCATGGAGGAGCCCCTGGTGATAGAGAAGGACCTGC
  CCCACTATTTCTAGCTCCACAAGTGTGTGGCCCCGCCCGAGCCCCTGCCCACGCTCTTGG
  AGCCTTTCACCGGCACTCATGACGGCCTGCCTGCCAACCTGCTGGTGGGGCAGACCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002085 unedited
  TTTATTCCCACAAGTAGCCAGGGGTGGGGGCGCCGAGCCAAGCCCAGCAGGCCATGGGAC
  CTTCCTCCGGCGGGGTGCACGCTGGATTTTCGGGTCTGCCCCACCAGCAGGTTTGCAGGC
  AGGCCGTCATGAGTGCCGGTGGAAGGCTCCAAGGGCGTGGGCAGGGGCTCGGGCGGGGCC
  ACACACTTGTGGAGCTAGAAATAGTGGGGCAGGTCCTTCTCTATCACCAGGGGCTCCTCC
  ATGGGTCCGTAGCGCTTCACCACGCAGCCGTTCTTGTCGATGAGGAACTTGGTGAAGTTC
  CACTTGATGGCATTTCCCAGGATGCCCTTGCCCTTGGGTTGGATCTTCATCCACTTCCAC
  AGCGGGTGGGCGTCGTCCCCGTTCACGCAGATCTTGCTGAACATATCGAATTTGACGTTG
  TAGCCCGCGGCGAACTCTTTGATCTCTTCGTTACTCCCTGGCTCCTGCTTCCCGAACTGG
  TTACACGGGAAGGCCAGGATCCGCAAACCACACTCAGCGTATCGGGCGTGCAGGTCGACG
  AGCTGAGTGTAGTTTACTTCGGTCTTGCCTCACTGGGAGGCCACGTTGGTGACGATGCAC
  ACGAAGCCCCGGTACTTGTCCAGGTTAACCATGTGCCCGTCGATGTCCTTGGCGGAAACT
  CGTGCATGGAGCGCGCACAGCGCCAGTCGTCCTGGGACGCGCACGGNTCCTGNGACTTGG
  GACATCCGGGNATCTCCGGTCGCAAGCTGCGGTGACAGGGCTTNTTCCGCCGGCGCCGNG
  CCCTTCTGNNGCTGCACGCTGGGGCCCTTCCGCCGCCGAGGGGCTCGCGGCCGNCGNCTG
  CCCCGCTCTGGCACGNTGNCTGCNGCGCCCGGGGNNAGCTGCGCCGGGCGGCCATACCCG
  CCGTGTTCGCCCCTCTGGGTCCCCACCCTNGTGCCGATNCGGGCCGCCTTATGGAGCGTT
  ATACAAATTTGACGGTCCTAACGAGCTTTGCTTATTGACTCCCACGTTACCCTACCGNCA
  TTGCTG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002085
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002085.1, NP_002076.1
• RefSeq Size: 942 bp
• Locus ID: 2879
• UniProt ID: P36969
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Glutathione metabolism
• Gene Summary: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. [provided by RefSeq, Dec 2018]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A. A similar isoform in rat (L-form) has been shown to be localized to the mitochondria (PMID:9988735). CCDS Note: This CCDS ID represents a selenoprotein that is functionally sensitive to selenium levels (e.g., PMID:14642406). Position 73 of the protein is a selenocysteine residue, which is cotranslationally incorporated when a UGA codon, which is normally a stop codon, is recognized by a specific selenocysteyl-tRNA in conjunction with a stem-loop SECIS element in the 3' UTR.