Glutathione Peroxidase 3 (GPX3) (NM_002084) Human Untagged Clone

CAT#: SC118819

GPX3 (untagged)-Human glutathione peroxidase 3 (plasma) (GPX3) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: Glutathione Peroxidase 3
• Synonyms: GPx-P; GSHPx-3; GSHPx-P
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118819 sequence for NM_002084 edited (data generated by NextGen Sequencing)
  ATGGCCCGGCTGCTGCAGGCGTCCTGCCTGCTTTCCCTGCTCCTGGCCGGCTTCGTCTCG
  CAGAGCCGGGGACAAGAGAAGTCGAAGATGGACTGCCATGGTGGCATAAGTGGCACCATT
  TACGAGTACGGAGCCCTCACCATTGATGGGGAGGAGTACATCCCCTTCAAGCAGTATGCT
  GGCAAATACGTCCTCTTTGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGTACATT
  GAACTGAATGCACTACAGGAAGAGCTTGCACCATTCGGTCTGGTCATTCTGGGCTTTCCC
  TGCAACCAATTTGGAAAACAGGAACCAGGAGAGAACTCAGAGATCCTTCCTACCCTCAAG
  TATGTCCGACCAGGTGGAGGCTTTGTCCCTAATTTCCAGCTCTTTGAGAAAGGGGATGTC
  AATGGAGAGAAAGAGCAGAAATTCTACACTTTCCTAAAGAACTCCTGTCCTCCCACCTCG
  GAGCTCCTGGGTACATCTGACCGCCTCTTCTGGGAACCCATGAAGGTTCACGACATCCGC
  TGGAACTTTGAGAAGTTCCTGGTGGGGCCAGATGGTATACCCATCATGCGCTGGCACCAC
  CGGACCACGGTCAGCAACGTCAAGATGGACATCCTGTCCTACATGAGGCGGCAGGCAGCC
  CTGGGGGTCAAGAGGAAGTAA
  
  Clone variation with respect to NM_002084.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002084 unedited
  TGCGTCGAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTG
  CCCGAATTCGCACGAGGGGGATCAGGCAGCGGCTCAGGCGACCCTGAGTGTGCCCCCACC
  CCGCCATGGCCCGGCTGCTGCAGGCGTCCTGCCTGCTTTCCCTGCTCCTGGCCGGCTTCG
  TCTCGCAGAGCCGGGGACAAGAGAAGTCGAAGATGGACTGCCATGGTGGCATAAGTGGCA
  CCATTTACGAGTACGGAGCCCTCACCATTGATGGGGAGGAGTACATCCCCTTCAAGCAGT
  ATGCTGGCAAATACGTCCTCTTTGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGT
  ACATTGAACTGAATGCACTACAGGAAGAGCTTGCACCATTCGGTCTGGTCATTCTGGGCT
  TTCCCTGCAACCAATTTGGAAAACAGGAACCAGGAGAGAACTCAGAGATCCTTCCTACCC
  TCAAGTATGTCCGACCAGGTGGAGGCTTTGTCCCTAATTTCCAGCTCTTTGAGAAAGGGG
  ATGTCAATGGAGAGAAAGAGCAGAAATTCTACACTTTCCTAAAGAACTCCTGTCCTCCCA
  CCTCGGAGCTCCCTGGTACATCTGACCGCCTCTTCTGGGAACCCATGAAGGTCACGACAT
  CCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCCAGATGGTATACCCATCATGCGCTGGCA
  CCCACGGACCACGGTCAGCAACGTCAAGATGGACATCCCTGTCTACATGAGGCGGCCAGC
  AGCCCTGGGGGTCAAAGAGAAGTAAACTGAGGCCGTCTCATCCCATGTCCCACATGTAGG
  GGAGGAACTTTTGTTCAGGAAGAAATCCGTGTCTTCCACACACTATCTACCCATCACAGA
  CCCCTTTCCTATCACTCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002084 unedited
  NNNTTTTAGCTATGGACCGGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTT
  TTTTGCTGCAGAAAGGCTTTTACTGGGCAGACGGGGTGAGTCTCAAGCCAGTGGACCAGT
  GAGGGGTGAGGGCACGTCCTCCGAAGGAGCAGGGGTGGCATCCCTGCCCAGGGGCCTTAG
  CCTGAATGCACTAAGGGCTGGCCCCTCAGACAGGCTCAGGGGAGGTCCGCCCACAAGGGC
  TTTGGGCCCCTCCCTCATGAAGACGCCACCCCTGCCATGGGGTCCGTGGCCCTTGCTGTC
  ACATCTGCCTTGGTTGATCTCAGGGAGGTTGATCTCTCTCAGGGAGAACTGTGGGGTGGG
  GATGTAGCCTTGGGAGGGCCCTTCAGGAAGTAAGAGTGGGGGGTTGGGGAGTGTTNGTAN
  ACCCANAAACTTCTGGGGACGTCAGTCATAGTTACTAATATTTGGAGGCAGTGGGAGATG
  CTGGCCCCAAGGTTGAGGTATCAGTTAGAGCAGAACAATTGGACCTAGAGCTGGTTTTTC
  CTTTGGGTTTAGGTGTAAGTGAACTATTATCATTGGAGTGGAGAACTGGAGAGAAAGGGT
  TGTCACTGTCCAGCACTACACAGCTGCAGGCACACAGATGGTACACCATTCCAGAAAGAC
  ACATAGGCTGACATGTACTTGGNCACNNATGCNNACACACNCAATNANGCATACCTGGAG
  GCATGNGGGNAAACACCACATGCACCCACACCCCATGCCTGGCAGTACAAGAACTGTTGC
  ATTCATTTGTGCCAGGCTGGGCCCTGACAGAAGGAAAGGGGCTGTGTTGTTACACCCCCT
  GTCTGCAAACCCTCCTTTCTTAAAAAATCCTCCCCTCTGGGGCATCGCAGAACGGCTTAT
  TCCTTCCTCCAACCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002084
• Insert Size: 1660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002084.2, NP_002075.2
• RefSeq Size: 1856 bp
• Locus ID: 2878
• UniProt ID: P22352
• Cytogenetics: 5q33.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Arachidonic acid metabolism, Glutathione metabolism
• Gene Summary: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1.