Aspartate Aminotransferase (GOT1) (NM_002079) Human Untagged Clone

CAT#: SC118816

GOT1 (untagged)-Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aspartate Aminotransferase
• Synonyms: AST1; ASTQTL1; cAspAT; cCAT; GIG18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118816 sequence for NM_002079 edited (data generated by NextGen Sequencing)
  ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAG
  CTCACTGCCGACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCA
  TATCGCACGGATGACTGCCATCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAG
  ATTGCTAATGACAATAGCCTAAATCACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTC
  CGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGG
  GTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTA
  GCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC
  TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGC
  TACTGGGATGCAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAAT
  GCTCCTGAGTTCTCCATTGTTGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGAC
  CCAACTCCGGAGCAGTGGAAGCAGATTGCTTCTGTCATGAAGCACCGGTTTCTGTTCCCC
  TTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACCTGGAGAGAGATGCCTGGGCC
  ATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTTCTCCAAGAAC
  TTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC
  ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCC
  GCCCAGGGAGCACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGG
  ACAGGTAATGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCA
  CGACTAGAAGCCCTCAAAACCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATG
  TTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATCTGGTCAATGAAAAGCACATC
  TACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAATCTAGATTAC
  GTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAGTGA
  
  Clone variation with respect to NM_002079.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002079 unedited
  ACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCCAGACCTGACCTGCCAGCTCCGGG
  CGTGGGGTGAAATCTCTTGATTCCTAGTCTCTCGATATGGCACCTCCGTCAGTCTTTGCC
  GAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAGCTCACTGCCGACTTCAGGGAGGAT
  CCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCATATCGCACGGATGACTGCCATCCC
  TGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAGATTGCTAATGACAATAGCCTAAAT
  CACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTCCGGAGCTGTGCTTCTCGTCTTGCC
  CTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGGGTAGGAGGTGTGCAATCTTTGGGG
  GGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTAGCGCGTTGGTACAATGGAACAAAC
  AACAAGAACACACCTGTCTATGTGTCCTCACCAACCTGNGAGAATCACAATGCTGTGTTT
  TCCGCTGCTGGTTNTAAAGACATTCGGTCCTATCGCTACTGNGATGCAGAGAGAGAGGAT
  TGGACCTCCAGGGCTTCCTGAATGATCTGGAGAATGCTCCTGAGTTCTCCATTGNTGTCC
  TCCACGCCTGTGCACACCACCCAACTGGNGATGACCNCACTCNGNAGCAGTGGAAGCAGA
  TTGCTTCTGTCATGAAGCACCGTTTNNCTGTNCCCTTCTTTGACTCANCTATCAGGNCTT
  CGCATCTGGAAACCCTGAGAGAGATGCCCTGGCCATTCGCTATTTTGTGTCTGAAGGCTT
  CAGTTCTTCTGTGCCCAGTCCTCTNCAGAACTNCNGGCTCTACATGANAGAGTTCGGAAT
  CTGACTGTGGGTGGAAAAGACCTGANAGCTNCTTGCAGGTCCTTCCCAATGGGAAAGATC
  GTGCGATTACTTGTTCCATNCCCCGCCAGGAGACAGATTGGGGCCGACCCTCCTAACCTG
  ACCTTTGGGAAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002079 unedited
  AAACCCCCGGCCCNCCCTAACTCCTATATCCCCTGCTTTTACTTGANCCGCGGCACGCTA
  TTCTANATCGAGTTTTTTTTTTTTTTTTTTGCACAACATTTTTATTTTATTTTTTTAGGC
  GAAGTACAACACAATACAATGGCTCAAAAATATCAGAATGCACTACGCACATCACGAGTA
  AATACTGTCTGGTAAAACTTGTTTCAGTTAAATATGTACGTGCCCGCGCATGTCATGATT
  AAATATCCTTCTTACCACAGTCACCCTAAAGAACCAAAGCTTAGGACTAGGGACACACCC
  ATGCAGAAAGAGCAGGGAGACCAGACACTCTGGGTTGAGATGATGAATTTAATGCCGCAG
  CCGACACCCACATTCACACTTTGGCTCCTTTACACAACTTGATCTTTGGGACTACCAATT
  TGTACAGAGTTAAACACCACATAGAAGCACGCGGCCGCCACACACAACACTCCTTTTTAG
  TGAGAGGGACACCACCCCTATGTCTCATGATCCAAGCACTCTTTTATTCTTAAATAAAAA
  TCTTAATTTGCCTTTGCACCTCAAAGGCTCTAATCCCAGTCTTCAAATTCGTCTCAAGGG
  ATGTTCTCTTCATGTGGGGCTCGGTCCAAACCGCACGCTGCCTTACCACACCCGCCTTTC
  ACCTCCTCGCAGCGTCCCTCTACCCCCATGGCCATGTCCATGCACGTTTCGTCCCGCCAG
  CGACCCCCCCTTAACAGAAACTTCCTTTGGGGCCACTGGACCGGGTGGAGTTCCCTTCCC
  CTGCATTCTCGGTCACCTGTCTTTATGGCCTGGAGAGTGCCACCCCCACACTCCAATCTT
  TCCGGGCCAAACCCACTTTACGCCCGATTTCCACACCTCGCCAACCCCTTCCAATTCCTC
  TTTTACTTGGCTCAAAACTTACAACCGCTTCGGGGTTCACACCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002079
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002079.1, NP_002070.1
• RefSeq Size: 1941 bp
• RefSeq ORF: 1242 bp
• Locus ID: 2805
• UniProt ID: P17174
• Cytogenetics: 10q24.2
• Domains: aminotran_1_2
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]