HLA-DQB1 (NM_002123) Human Untagged Clone

CAT#: SC118796

HLA (untagged)-Human major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-DQB1
• Synonyms: CELIAC1; HLA-DQB; IDDM1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002123, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTTGGAAGAAGGCTTTGCGGATCCCCGGAGACCTTCGGGTAGCAACTGTCACCTTGATGCTGGCGA
  TGCTGAGCTCCCTACTGGCTGAGGGCAGAGACTCTCCCGAGGATTTCGTGTTCCAGTTTAAGGGCATGTG
  CTACTTCACCAACGGGACGGAGCGCGTGCGTCTTGTGACCAGATACATCTATAACCGAGAGGAGTACGCG
  CGCTTCGACAGCGACGTGGGGGTGTACCGCGCGGTGACGCCGCAGGGGCGGCCTGATGCCGAGTACTGGA
  ACAGCCAGAAGGAAGTCCTGGAGGGGACCCGGGCGGAGTTGGACACGGTGTGCAGACACAACTACGAGGT
  GGCGTTCCGCGGGATCTTGCAGAGGAGAGTGGAGCCCACAGTGACCATCTCCCCATCCAGGACAGAGGCC
  CTCAACCACCACAACCTGCTGGTCTGCTCGGTGACAGATTTCTATCCAGGCCAGATCAAAGTCCGGTGGT
  TTCGGAATGATCAGGAGGAGACAGCCGGCGTTGTGTCCACCCCCCTTATTAGGAATGGTGACTGGACTTT
  CCAGATCCTGGTGATGCTGGAAATGACTCCCCAGCGTGGAGATGTCTACACCTGCCACGTGGAGCACCCC
  AGCCTCCAGAGCCCCATCACCGTGGAGTGGCGGGCTCAGTCTGAATCTGCCCAGAGCAAGATGCTGAGTG
  GCGTTGGAGGCTTCGTGCTGGGGCTGATCTTCCTTGGGCTGGGCCTTATCATCCGTCAAAGGAGTCAGAA
  AGGGCTTCTGCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002123 unedited
  ACCTTGGATTTTGTACACGATTACTATAGGCGGCCGCGAATTCGGCACCAGGTATGACTA
  CCACTACTTTTCCCTTCGTCTCATTATGTCTTGGAAGAAGTCTTTGCGGATCCCCGGAGA
  CCTTCGGGTAGCAACTGTCACCTTGATGCTGGCGATCCTGAGCTCCTCACTGGCTGAGGG
  CAGAGACTCTCCCGAGGATTTCGTGTACCAGTTTAAGGGCCTGTGCTACTTCACCAACGG
  GACGGAGCGCGTGCGGGGTGTGACCAGACACATCTATAACCGAGAGGAGTACGTGCGCTT
  CGACAGCGACGTGGGGGTGTACCGGGCAGTGACGCCGCAGGGGCGGCCTGTTGCCGAGTA
  CTGGAACAGCCAGAAGGAAGTCCTGGAGGGGGCCCGGGCGTCGGTGGACAGGGTGTGCAG
  ACACAACTACGAGGTGGCGTACCGCGGGATCCTGCAGAGGAGAGTGGAGCCCACAGTGAC
  CATCTCCCCATCCAGGACAGAGGCCCTCAACCACCACAACCTGCTGATCTGCTCGGTGAC
  AGATTTCTATCCAAGCCAGATCAAAGTCCGGTGGTTTCGGAATGATCAGGAGGAGACAGC
  CGGCGTTGTGTCCACCCCCCTCATTAGGAACGGTGACTGGACCTTCCAGATCCTGGTGAT
  GCTGGAAATGACTCCCCAGCGTGGAGATGTCTACACCTGCCACGTGGAGCACCCCAGCCT
  CCAGAGCCCCATCACCGTGGAGTGGCGGGCTCAGTCTGAATCTGCCCAGATCAGATGCTG
  AGTGGCGTTGGGAGCTTCGTGCTGGGGCTGATCTTCCTTGGGCTTGGNCCTATCATCCGT
  CAAAGGAGTCGGANAGGGCTTCTGCACTGACTCCTGAGACTGTTTTAACTAAGACTGGNT
  ATCACTCTNCTGTGATGCCTGCTGTCCCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002123 unedited
  AAAGCTTGGAACGCGGCACGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTTTTA
  ACTCAGGATCATGTTTAATTATGTAAAAAAGCTCTGAAGTCAGGTAATGTTTTTCATGTG
  CTTCTCTTGAGCAGTCTGAGGAGAGAACAGAAACAGAATCCCCTTGGGGCCTGAGTAGAC
  GCAGCTGGCCATGTACAGGCAGTGGCTCTGGGTCAGTGCAGGAAGCAGAGTCACAGCCAG
  CGCCTTGGGGTGGGGATGAAAGGAGATGACCTGGTGGTTGCGTGACAGCCACTGTAGGAC
  TTTGATCTCAGGGGGACAAGCTGACACAGGCAGCTGGGAATTCTGGGCAGGGACAAGCAG
  GCATCACAGAAGAGTGATAACCAGTCTTAGTTAAAACAGTCTCAGGAGTCAGTGCAGAAG
  CCCTTTCCGACTCCTTTGACGGATGATAAGGCCAAGCCCAAGGAAGATCAGCCCCAGCAC
  GAAGCCTCCAACGCCACTCAGCATCTTGCTCTGGGCAGATTCAGACTGAGCCCGCCACTC
  CACGGTGATGGGGCTCTGGAGGCTGGGGTGCTCCACGTGGCAGGTGTAAACATCTCCACG
  CTGGGGAGTCATTTCCAGCATCACCAGGATCTGGAAGGTCCAGTCACCGTTCCTAATGAA
  GGGGGTGGACACAACGCCGGCTGGCTCCTCCTGATCATTCCAAAACCCCCGGACTTTGAT
  CTGGCCTGGATAGAAAATTGNCCCCGAGCAAAACANCAGGTGGGGGGGGTTGAAGGCCCT
  TGTTCCTGGCGGGGAAAACGGCCCTTGGGGCTTCCTCTCTCCTTTGCAGAACCCCCTGAT
  CCCCCCCTTTTTCTGCGGTTGCACCCCCTGCCCCAAAAGCCCGCGGCCCATCAGAACTGC
  CTTTGCCCGTGCCANCCTCCGCAACGCGTTCCTCCCTCCCCTTTCTCTTTTCCCACACCC
  CATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002123
• Insert Size: 1180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002123.2, NP_002114.2
• RefSeq Size: 1190 bp
• RefSeq ORF: 786 bp
• Locus ID: 3119
• UniProt ID: P01920
• Cytogenetics: 6p21.32
• Domains: MHC_II_beta, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) is the predominant transcript and encodes isoform 1. This transcript represents the DQB1*06:02:01:01 allele of the HLA-DQB1 gene, as represented in the assembled chromosome 6 in the primary assembly of the reference genome.