HLADQA1 (HLA-DQA1) (NM_002122) Human Untagged Clone

CAT#: SC118795

HLA (untagged)-Human major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLADQA1
• Synonyms: CELIAC1; DQ-A1; DQA1; HLA-DQA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118795 sequence for NM_002122 edited (data generated by NextGen Sequencing)
  ATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCTCTGACCACCGTGATGAGCCCC
  TGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCTTGTGGTGTAAACTTGTACCAG
  TTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGATGGAGATGAGCAGTTCTACGTG
  GACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAGTTCAGCAAATTTGGAGGTTTT
  GACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAACACAACTTGAACATCATGATT
  AAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCTGAGGTCACAGTGTTTTCCAAG
  TCTCCCGTGACACTGGGTCAGCCCAACACCCTCATCTGTCTTGTGGACAACATCTTTCCT
  CCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCAGTCACAGAAGGTGTTTCTGAG
  ACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAGATCAGTTACCTCACCTTCCTC
  CCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCACTGGGGCCTGGACCAGCCTCTT
  CTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCAGAGCTCACAGAGACTGTGGTC
  TGTGCCCTGGGGTTGTCTGTGGGCCTCATGGGCATTGTGGTGGGCACTGTCTTCATCATC
  CAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGGCCATTGTGA
  
  Clone variation with respect to NM_002122.3
  396 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002122 unedited
  CGGTCAGCATTTGTATACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCCAACTGC
  TGAGGCTGCCTTGGGAAGAGGATGATCCTAAACAAAGCTCTGCTGCTGGGGGCCCTCGCT
  CTGACCACCGTGATGAGCCCCTGTGGAGGTGAAGACATTGTGGCTGACCACGTTGCCTCT
  TGTGGTGTAAACTTGTACCAGTTTTACGGTCCCTCTGGCCAGTACACCCATGAATTTGAT
  GGAGATGAGCAGTTCTACGTGGACCTGGAGAGGAAGGAGACTGCCTGGCGGTGGCCTGAG
  TTCAGCAAATTTGGAGGTTTTGACCCGCAGGGTGCACTGAGAAACATGGCTGTGGCAAAA
  CACAACTTGAACATCATGATTAAACGCTACAACTCTACCGCTGCTACCAATGAGGTTCCT
  GAGGTCACAGTGTTTTCCAAGTCTCCCGTGACACTGGGTCAGCCCAACACCCTCATCTGT
  CTTGTGGACAACATCTTTCCTCCTGTGGTCAACATCACATGGCTGAGCAATGGGCAGTCA
  GTCACAGAAGGTGTTTCTGAGACCAGCTTCCTCTCCAAGAGTGATCATTCCTTCTTCAAG
  ATCAGTTACCTCACCTTCCTCCCTTCTGCTGATGAGATTTATGACTGCAAGGTGGAGCAC
  TGGGGCCTGGACCAGCCTCTTCTGAAACACTGGGAGCCTGAGATTCCAGCCCCTATGTCA
  GAGCTCACAGAGACTGTGGTCTGTGCCCTGNGGTTGTCTGTGGGCCTCATGGGCATTGTG
  GTGGGCACTGTCTTCATCATCCAAGGCCTGCGTTCAGTTGGTGCTTCCAGACACCAAGGG
  CCATTGTGAATCCATCCTGAANGGAAGGTGCATCGCCATCTACAGGAGCAGAAGAATGGA
  CTTGCTAAATGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002122 unedited
  ATGGACCGCGCGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTCATAAAAGGA
  ATTTATTGCTTCCTTGGAGATTTTAGATGGAGGTTACTGAGGAATTAGGTAGCCGGGTGG
  CTTACCCCAGGCATGTTTTGTAGGTAACATTTGAGAAAAGAAAAAAAAAATCAGGAGGTC
  AGGGAAAGAATGTAAAGGCATTTGTGAGCTCTGAGGGGATATAGCAGCTTAAGTCCCAGA
  GAAAAGGTGAGAGGAGAAATATTTGGAGGAGAAAAGGGATATGATAAATCGGGCCAGAGA
  ATAGTGCTAGGTCATTTAGCAAGTCCATTCTTCTGCTCCTGTAGATGGCGATGCACCTTC
  CCTTCCAGGATGGGATTCACAATGGCCCTTGGTGTCTGGAAGCACCAACTGAACGCAGGC
  CTTGGATGATGAAGACAGTGCCCACCACAATGCCCATGAGGCCCACAGACAACCCCAGGG
  CACAGACCACAGTCTCTGTGAGCTCTGACATAGGGGCTGGAATCTCAGGCTCCCAGTGTT
  TCAGAAGAGGCTGGTCCAGGCCCCAGTGCTCCACCTTGCAGTCATAAATCTCATCAGCAG
  AAGGGAGGAAGGTGAGGTAACTGATCTTGAAGAAGGAATGATCACTCTTGGAGAGGAAGC
  TGGTCTCAGAAACACCTTCTGTGACTGACTGCCCATTGCTCAGCCATGTGATGTTGACCA
  CAGGAGGAAAGATGTTGTCCACAAGACAGATGAGGGTGTTGGGCTGACCCAGTGTCACGG
  GAGACTTGGAAAACACTGTGACCTCAGGAACCTCATTGGTAGCAGCGGTAGAGNTGTAGC
  GTTTAATCATGATGTTAAAGTGTGTTTTGCCACACCCATGTTCTCAGTGCACCCTGCGGG
  TCAAACCTCCAATTNGCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002122
• Insert Size: 1240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002122.3, NP_002113.2
• RefSeq Size: 1542 bp
• RefSeq ORF: 768 bp
• Locus ID: 3117
• UniProt ID: P01909
• Cytogenetics: 6p21.32
• Domains: MHC_II_alpha, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]