SFRP4 (NM_003014) Human Untagged Clone

CAT#: SC118247

SFRP4 (untagged)-Human secreted frizzled-related protein 4 (SFRP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFRP4
• Synonyms: FRP-4; FRPHE; FRZB-2; PYL; sFRP-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118247 sequence for NM_003014 edited (data generated by NextGen Sequencing)
  ATGTTCCTCTCCATCCTAGTGGCGCTGTGCCTGTGGCTGCACCTGGCGCTGGGCGTGCGC
  GGCGCGCCCTGCGAGGCGGTGCGCATCCCTATGTGCCGGCACATGCCCTGGAACATCACG
  CGGATGCCCAACCACCTGCACCACAGCACGCAGGAGAACGCCATCCTGGCCATCGAGCAG
  TACGAGGAGCTGGTGGACGTGAACTGCAGCGCCGTGCTGCGCTTCTTCCTCTGTGCCATG
  TACGCGCCCATTTGCACCCTGGAGTTCCTGCACGACCCTATCAAGCCGTGCAAGTCGGTG
  TGCCAACGCGCGCGCGACGACTGCGAGCCCCTCATGAAGATGTACAACCACAGCTGGCCC
  GAAAGCCTGGCCTGCGACGAGCTGCCTGTCTATGACCGTGGCGTGTGCATCTCGCCTGAA
  GCCATCGTCACGGACCTCCCGGAGGATGTTAAGTGGATAGACATCACACCAGACATGATG
  GTACAGGAAAGGCCTCTTGATGTTGACTGTAAACGCCTAAGCCCCGATCGGTGCAAGTGT
  AAAAAGGTGAAGCCAACTTTGGCAACGTATCTCAGCAAAAACTACAGCTATGTTATTCAT
  GCCAAAATAAAAGCTGTGCAGAGGAGTGGCTGCAATGAGGTCACAACGGTGGTGGATGTA
  AAAGAGATCTTCAAGTCCTCATCACCCATCCCTCGAACTCAAGTCCCGCTCATTACAAAT
  TCTTCTTGCCAGTGTCCACACATCCTGCCCCATCAAGATGTTCTCATCATGTGTTACGAG
  TGGCGTTCAAGGATGATGCTTCTTGAAAATTGCTTAGTTGAAAAATGGAGAGATCAGCTT
  AGTAAAAGATCCATACAGTGGGAAGAGAGGCTGCAGGAACAGCGGAGAACAGTTCAGGAC
  AAGAAGAAAACAGCCGGGCGCACCAGTCGTAGTAATCCCCCCAAACCAAAGGGAAAGCCT
  CCTGCTCCCAAACCAGCCAGTCCCAAGAAGAACATTAAAACTAGGAGTGCCCAGAAGAGA
  ACAAACCCGAAAAGAGTGTGA
  
  Clone variation with respect to NM_003014.3
  786 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003014 unedited
  AGGTTACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAGGATTTC
  TTCCTCGGCGAAGGACAGCGAAAGAGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTGTCTG
  CCGGGGTCGCAGCGCGAGAGGGCAGTGCCATGTTCCTCTCCATCCTAGTGGCGCTGTGCC
  TGTGGCTGCACCTGGCGCTGGGCGTGCGCGGCGCGCCCTGCGAGGCGGTGCGCATCCCTA
  TGTGCCGGCACATGCCCTGGAACATCACGCGGATGCCCAACCACCTGCACCACAGCACGC
  AGGAGAACGCCATCCTGGCCATCGAGCAGTACGAGGAGCTGGTGGACGTGAACTGCAGCG
  CCGTGCTGCGCTTCTTCCTCTGTGCCATGTACGCGCCCATTTGCACCCTGGAGTTCCTGC
  ACGACCCTATCAAGCCGTGCAAGTCGGTGTGCCAACGCGCGCGCGACGACTGCGAGCCCC
  TCATGAAGATGTACAACCACAGCTGGCCCGAAAGCCTGGCCTGCGACGAGCTGCCTGTCT
  ATGACCGTGGCGTGTGCATCTCGCCTGAAGCCATCGTCACGGACCTCCCGGAGGATGTTA
  AGTGGATAGACATCACACCAGACATGATGGTACAGGAAAGGCCTCTTGATGTTGACTGTA
  AACGCCTAAGCCCCGATCGGTGCAAGTGTAAAAAGGTGAAGCCAACTTTGGCAACGTATC
  TCAGCAAAACTACAGCTATGTTATTCATGCAAAAATAAAGCTGTGCAGAGGAGTGGGCTG
  CATGAAGTCACAACGGTGGTGGATGTAAAAGAGATCTTNCAGTNCTCATCACCCATCCCT
  CGAACTTCAGTCCCGCTCATTACAATTCTTTCTGGCAGTGTCCACACATCCTGCCCCATC
  AAGATGTTCTCATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003014 unedited
  GGCCGCATTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCTCTCCTTTTTATTTATTTT
  TCTTTATTATTATTTTTGTTTTTAGAGGTTCTAGCTAAAAACAGAGCTGAAGTCATTGTA
  AAAAAGACACATTATAATTTTCCATATGCTACTTCTCTAGATTTTTCAAAAAATCCCCTT
  TCATCACAAAAATCCTTCTGCAGACCATAAACACATAAAGATCTTTTAGACATTCATTCC
  ACTACTGTTCCTTCTTAGAAGCATTATATTTTCGATCACATTTTAAAACATCACTAACAT
  TGCAACAACAGATATCAAAAGGTAATAAAACATACTGTAAAAAAATTACGTGTTTTGTTT
  GAAATATGGCATTTATTTTACAATGCACATATTAGGTCGAATTGTAACAAGCATTATTTT
  CCCTACTCTTCTAGAGTATGCACACAGGTTACTCTGAATGCAATGCAATAAGCCTTTTCC
  ACCAGCTTTAACTCACCTTCTGTACCAAAGGGCAAACCTACCACTATGGCTTGTGATGGC
  TTACAAAGAAAAACTGAAGCATAGCCTTAAGAAAAATGCTGCAAGATGTGTCTATGAAGA
  GCACTGCAGTGAGTTGTTAGGGCAAGGGGCACATGGCCTTACATAGGCTGTCCCAGGCAA
  TGCCCAGCCTCATCCTGTAAGGAAGTCGGAAGTCTCCGCTTTGGAAACTAGTTAGCTCAC
  ACTCTCTTCGGNTTTGTTCTCTTCTGGCACTCCTANGTCTATGGTCTTCTTGGGACTGGC
  TGGNTTAGGAGCAGGAGGCTTTCCCTTTGTTTGGGGGGATACTACGACTGGNTGCGCCGG
  CTGTTTTCTTTCTGTCCTGAACTGTNTACGCTGTTCCTGCAGCTCTCTTCCACTGTATGG
  ATCGTTTACTAGCTGATCNTCCATTTTTAACTAGCAATTTCAAGAGCATCTCCCTGTACG
  CACTCGTACCCATGAGAAACATCTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003014
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003014.2, NP_003005.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1041 bp
• Locus ID: 6424
• UniProt ID: Q6FHJ7
• Cytogenetics: 7p14.1
• Domains: FRI, NTR
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Gene Summary: Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]