Spermidine synthase (SRM) (NM_003132) Human Untagged Clone

CAT#: SC118178

SRM (untagged)-Human spermidine synthase (SRM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Spermidine synthase
• Synonyms: PAPT; SPDSY; SPS1; SRML1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118178 sequence for NM_003132 edited (data generated by NextGen Sequencing)
  ATGGAGCCCGGCCCCGACGGCCCCGCCGCCTCCGGCCCCGCCGCCATCCGCGAGGGCTGG
  TTCCGCGAGACCTGCAGCCTGTGGCCCGGCCAGGCCCTGTCGCTGCAGGTGGAGCAGCTG
  CTCCACCACCGGCGCTCGCGCTACCAGGACATCCTCGTCTTCCGCAGTAAGACCTATGGC
  AACGTGCTGGTGTTGGACGGTGTCATCCAGTGCACGGAGAGAGACGAGTTCTCCTACCAG
  GAGATGATCGCCAACCTGCCTCTCTGCAGCCACCCCAACCCGCGAAAGGTGCTGATCATC
  GGGGGCGGAGATGGAGGTGTCCTGCGGGAGGTGGTGAAGCACCCCTCCGTGGAGTCCGTG
  GTCCAGTGTGAGATCGACGAGGATGTCATCCAAGTCTCCAAGAAGTTCCTGCCAGGCATG
  GCCATTGGCTACTCTAGCTCGAAGCTGACCCTACATGTGGGTGACGGTTTTGAGTTCATG
  AAACAGAATCAGGATGCCTTCGACGTGATCATCACTGACTCCTCAGACCCCATGGGCCCC
  GCCGAAAGTCTCTTCAAGGAGTCCTATTACCAGCTCATGAAGACAGCCCTCAAGGAAGAT
  GGTGTCCTCTGCTGCCAGGGCGAGTGCCAGTGGCTGCACCTGGACCTCATCAAGGAGATG
  CGGCAGTTCTGCCAGTCCCTGTTCCCCGTGGTGGCCTATGCCTACTGCACCATCCCCACC
  TACCCCAGCGGCCAGATCGGCTTCATGCTGTGCAGCAAGAACCCGAGCACGAACTTCCAG
  GAGCCGGTGCAGCCGCTGACACAGCAGCAGGTGGCGCAGATGCAGCTGAAGTACTACAAC
  TCCGACGTGCACCGCGCCGCCTTTGTGCTGCCCGAGTTTGCCCGCAAGGCCCTGAATGAT
  GTGAGCTGA
  
  Clone variation with respect to NM_003132.2
  102 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003132 unedited
  TTACATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGGTTGTGCTGCG
  GCGCCGCGCGGCCTGCAGTCCCGGGCCCGCGCCCCGCGCCGCCCGCCCGCCCGCCATGGA
  GCCCGGCCCCGACGGCCCCGCCGCCTCCGGCCCCGCCGCCATCCGCGAGGGCTGGTTCCG
  CGAGACCTGCAGCCTGTGGCCCGGCCAGGCCCTGTCGCTGCAGGTGGAGCAGCTGCTCCA
  CCACCGGCGCTCGCGCTACCAGGACATCCTCGTCTTCCGCAGTAAGACCTATGGCAACGT
  GCTGGTGTTGGACGGTGTCATCCAGTGCACGGAGAGAGACGAGTTCTCCTACCAGGAGAT
  GATCGCCAACCTGCCTCTCTGCAGCCACCCCAACCCGCGAAAGGTGCTGATCATCGGGGG
  CGGAGATGGAGGTGTCCTGCGGGAGGTGGTGAAGCACCCCTCCGTGGAGTCCGTGGTCCA
  GTGTGAGATCGACGAGGATGTCATCCAAGTCTCCAAGAAGTTCCTGCCAGGCATGGCCAT
  TGGCTACTCTAGCTCGAAGCTGACCCTACATGTGGGTGACGGTTTTGAGTTCATGACACA
  GAATCANGATGCCTTCGACGTGATCATCACTGACTCCTCAGACCCCATGGGCCCCGCCGA
  AAGTCTCTTCAAGGAGTCCTATTACCAGCTCATGAAGACAGCCCCTCAAGGAGATGGTGT
  CCTCTGCCTGCCACGGCGAGTGCCCATGGCTGCACCTGGACCTCATTCAAGAGATGCCGG
  CAGTTCTGCCAGTCCCCTGTTCCCCCTTGGTGGCCTATGGCCTACTGTACCCATCCCCAC
  CTACCCCAGNGGNCCAGATCGGCTTCTTGCTGGGGCGCAAGAACCCCGGACACGAACTTT
  CCAGAACCCGGGGCAACCTTGAACCACCACCAAGTGGCGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003132 unedited
  ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTATAAATACACG
  TGTTTGGTGAGTGAGGGGCAACAGAAGGCAGAGAGATGGCGCTGTACACAGCTGGTATAG
  GCTTGGAGGTGGAACGCCAGAGAGACAGACACACAGACAGTCCGCCCAGCAGGGCAGGCC
  GGGCAGCATTCTGGGGCTTGTAACACTTGGTTGGTGGGCGAGAGCCAGCAGGAGGTCCGG
  CCCGGGGCTGGAGGGGCCGAGGCACCCCGCAGGCTCCAAGGTCCGAGGTCCTGGGTGGCA
  TCAGTGGTGGCGCCTGGGCTCAGCTCACATCATTCAGGGCCTTGCGGGCAAACTCGGGCA
  GCACAAAGGCGGCGCGGTGCACGTCGGAGTTGTAGTACTTCAGCTGCATCTGCGCCACCT
  GCTGCTGTGTCAGCGGCTGCACCGGCTCCTGGAAGTTCGTGCTCGGGTTCTTGCTGCACA
  GCATGAAGCCGATCTGGCCGCTGGGGTAGGTGGGGATGGTGCAGTAGGCATAGGCCACCA
  CGGGGAACAGGGACTGGCAGAACTGCCGCATCTCCTTGATGAGGTCCAGGTGCAGCCACT
  GGCACTCGCCCTGGCAGCAGAGGACACCATCTTCCTTTGAGGCTGTCTTCATGAGCTGGT
  AATAGGACTCCTTGAAGAGACTTTCGGCGGGGCCCATGGGGTCTGNAGAGTCAGTGATGA
  TCACGTCGAAGGCATCCTGATTCTGTTTCATGAACTCAAAACCGTCACCCACATTGTAGG
  GNTCAGCTTCGAGCTANAAGTAGCCAATGGCCATGCCCTGGCAGGAACTTCTTGGNAGAC
  TTGGATGACATCCTCGTCGATCTACACTGGACCACGACTCCCGGAAGGGTGCTTACCACC
  TCCCCAGGGAACCNCATTTTCGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003132
• Insert Size: 1310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003132.2, NP_003123.2
• RefSeq Size: 1273 bp
• RefSeq ORF: 909 bp
• Locus ID: 6723
• UniProt ID: P19623
• Cytogenetics: 1p36.22
• Domains: Spermine_synth
• Protein Pathways: Arginine and proline metabolism, beta-Alanine metabolism, Cysteine and methionine metabolism, Glutathione metabolism, Metabolic pathways
• Gene Summary: The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthase is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. This enzyme catalyzes the conversion of putrescine to spermidine using decarboxylated S-adenosylmethionine as the cofactor. [provided by RefSeq, Jul 2008]