SHARP2 (BHLHE40) (NM_003670) Human Untagged Clone

CAT#: SC117854

BHLHE40 (untagged)-Human basic helix-loop-helix family, member e40 (BHLHE40)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHARP2
• Synonyms: BHLHB2; Clast5; DEC1; HLHB2; SHARP-2; SHARP2; STRA13; Stra14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003670, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCGGATCCCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTGGAGCACGGAG
  ACCTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGACGGGGAATAAAGCGGAGCGA
  GGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATCGAGAAAAAGAGACGTGACCGGATTAACGAG
  TGCATCGCCCAGCTGAAGGATCTCCTACCCGAACATCTCAAACTTACAACTTTGGGTCACTTGGAAAAAG
  CAGTGGTTCTTGAACTTACCTTGAAGCATGTGAAAGCACTAACAAACCTAATTGATCAGCAGCAGCAGAA
  AATCATTGCCCTGCAGAGTGGTTTACAAGCTGGTGAGCTGTCAGGGAGAAATGTCGAAACAGGTCAAGAG
  ATGTTCTGCTCAGGTTTCCAGACATGTGCCCGGGAGGTGCTTCAGTATCTGGCCAAGCACGAGAACACTC
  GGGACCTGAAGTCTTCGCAGCTTGTCACCCACCTCCACCGGGTGGTCTCGGAGCTGCTGCAGGGTGGTAC
  CTCCAGGAAGCCATCAGACCCAGCTCCCAAAGTGATGGACTTCAAGGAAAAACCCAGCTCTCCGGCCAAA
  GGTTCGGAAGGTCCTGGGAAAAACTGCGTGCCAGTCATCCAGCGGACTTTCGCTCACTCGAGTGGGGAGC
  AGAGCGGCAGCGACACGGACACAGACAGTGGCTATGGAGGAGAATCGGAGAAGGGCGACTTGCGCAGTGA
  GCAGCCGTGCTTCAAAAGTGACCACGGACGCAGGTTCACGATGGGAGAAAGGATCGGCGCAATTAAGCAA
  GAGTCCGAAGAACCCCCCACAAAAAAGAACCGGATGCAGCTTTCGGATGATGAAGGCCATTTCACTAGCA
  GTGACCTGATCAGCTCCCCGTTCCTGGGCCCACACCCACACCAGCCTCCTTTCTGCCTGCCCTTCTACCT
  GATCCCACCTTCAGCGACTGCCTACCTGCCCATGCTGGAGAAGTGCTGGTATCCCACCTCAGTGCCAGTG
  CTATACCCAGGCCTCAACGCCTCTGCCGCAGCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTC
  CCTTGCTCATGCCCCAGAGACTCCCTTCTCCCTTGCCAGCTCATCCGTCCGTCGACTCTTCTGTCTTGCT
  CCAAGCTCTGAAGCCAATCCCCCCTTTAAACTTAGAAACCAAAGACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003670 unedited
  CAGCATTTTGTAATCACGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGCCGCCAG
  TCTGTGCGCTGAGTCGGAGCCAGAGGCCGCGGGGACACCGGGCCATGCACGCCCCCAACT
  GAAGCTGCATCTCAAAGCCGAAGATTCCAGCAGCCCAGGGGATTTCAAAGAGCTCAGACT
  CAGAGGAACATCTGCGGAGAGACCCCCGAAGCCCTCTCCAGGGCAGTCCTCATCCAGACG
  CTCCGCTAGTGCAGACAGGAGCGCGCAGTGGCCCCGGCTCGCCGCGCCATGGAGCGGATC
  CCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTGGAGCACGGAGAC
  CTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGACGGCGAATAAAG
  CGGAGCGAGGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATCGAGAAAAAGAGA
  CGTGACCGGATTAACGAGTGCATCGTCCAGCTGAAGGATCTCCTACCCGAACATCTCANA
  CTTACAACTTTGGGTCACTTGGAAAAAGCAGTGGTTCTTGAACTTACCCTTGAAGCATGT
  GAAAGCACTAACAAACCTAATTGATCAGCATCAGCAGAAAATCATTGCCCTGCAGAGTGG
  TTTACCAGCTGGTGAGCTGTCACGGAGCAATGTCGAAACAGGTCAAGAGATGTTTTGCTC
  AGGTTTCAGACCTGTGCCCCGGAGGTGCTTTAGCATCTGCCCAGCCCGAAAAACTCGGGA
  CCTGAAGTCTTCGCAGTTGTCCCCCCCCTCCCCCGGTGGGCTCTGAACTGCTGCAGGTTG
  GGCCCTCCAGGAACCATCCCGACCAGCTTCCCAAGAATGGCACTCAAGAAAAACCAGTTC
  TTCGGCCAAGGTTCGGAGGGCCGGGGAAAACCGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003670 unedited
  ACGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTCCCA
  GGAAAAAAAATTATTTTATTAAGTATTTTAAAACAACTACTTAACATTTACTCATAAATA
  AAAATATTTACAATTTTACACCTTCAGGAAGGCTCCAAAATATAAACACTGTACCTCTCC
  CTAAAAAAAAAAAAAATTATTCTTCTCTTCAAAAACAGGAATACATTCATTTTTTCTCAC
  TGGGGGAATCAAGTATTTATCCAAATAAACATCTGAAACATTTCCCTTTTTAATATATTA
  ATATAAAATATATTTATAACAGCTTTACAAATAAAGGCACCGACTTATTCCCAAATTAAA
  ACCGGGAAAAGAAAAAGTTAAAACACGATCAGCAATCAGGCATAGGGCATTTTGAAAAGC
  TGCTGCGGAGCTGGCTGGACACATCGGGAAAACAAGTACTCTGGACCAAAAATAAAACTT
  CTTTGAAGGAAACGGAATCCTTTTTAGCAATAAGGACACTCCATATGCCGTTGGGCAAGA
  GGAATTACCCTACAAAAAGGATCTGGCGGTGCCAAGGCTTGGTTAGGCCTTTGAGAACTA
  ACCTTGGTAAAAGGAGTTTAGGATGGAATTGAAAACGACTCATTTCTGTTTGAGGGGGCA
  GCTTTTTACTAACCTTTGGCACCTTGGGGGCTCCCTCGGGGTGCCCGATTTCTAAACCTG
  GTTTGGTTCCTGCCAGGAAGACTAATGTTAGCCCTAAACAGGGCCAAAACCCAGGGACTC
  CTCCTCCTCCGGGCATCTAACTTGACAAGGGCCCATTTTAATTCCTACAAGCTATCCCTC
  AAAGCCCGAGGGGAACCCATTTTTTTTTTTCGAGGAGGGGTGGGAAAACCTGGGAAACCT
  CACACCATACGTGTTTGCAGGCCGCTCCTCCCATGTAAAAAACCTGCCCGACGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003670
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003670.1, NP_003661.1
• RefSeq Size: 2922 bp
• RefSeq ORF: 1239 bp
• Locus ID: 8553
• UniProt ID: O14503
• Cytogenetics: 3p26.1
• Domains: HLH, ORANGE
• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]